Interference-free Determination of Indole-3-Acetic Acid in Two Real Systems Using Second-order Calibration Method Coupled with Excitation-emission Matrix Fluorescence

Abstract

In this work, a simple and practicable method that combines excitation-emission matrix (EEMs) fluorescence with a second-order calibration method based on parallel factor analysis-alternative least-squares (PARAFAC-ALS) algorithm was developed for the direct interference-free determination of indole-3-acetic acid (IAA) in two real systems, coconut water (CW) and coconut milk (CM). Although the excitation and emission profiles of IAA heavily overlapped with that of unknown interferents in the complex real systems, the actual contents and satisfactory recoveries were still obtained successfully. The contents of IAA in CW and CM were 10.8 ± 0.3 and 4.9 ± 0.2 μg mL -1 , respectively, which were consistent with those reported by LC-MS/MS assays in the reference material. The average spike recoveries of IAA in the validation set based on CW and CM were 102.1 ± 3.2 and 98.0 ± 1.9%, respectively. In addition, routine experiments were performed for establishing the validity of the assay to internationally accepted criteria.

Efficacious long-term cooling and freezing of Sapajus apella semen in ACP-118®

“…This is probably due the medium composition. TES-TRIS contains less buffers and fructose than CWS and ACP-118 ® which are rich in membrane stabilizers (Tan et al, 2014) such as potassium, sodium, calcium, magnesium, glucose and sucrose (Hincha et al, 2006;Yan et al, 2014). Semen dilution results in a decreased concentration of natural antioxidants present in the seminal plasma which results in a redox system imbalance, stress, and subsequent impairment of sperm motility, viability and capacity for fertilization (Agarwal et al, 2006;De Lamirande and O'Flaherty, 2008).…”