Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization

Pournaghi-Azar, M.H.; Ahour, F.; Hejazi, Mohammad Saeid

doi:10.1007/s00216-010-3875-5

Cited by 43 publications

(20 citation statements)

References 39 publications

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“…The calculated detection limit was 5.7× 10 −11 mol L −1 , although the linear range was 1-50 × 10 −9 mol L −1 and neither PCR nor real samples were used. This group also reported [51] use of the same PNA probe for [34] direct detection of the complementary sequence present in dsDNA oligos via triplex formation, achieving a detection limit of 1.8×10 −12 mol L −1 under the same conditions. Furthermore, they have recently reported the detection of SNPs in different PCR samples with high sensitivity (detection limit 4.8×10 −12 mol L −1 ), although the hybridization time was too long-up to 15 h was needed [52].…”

Section: Electrochemicalmentioning

confidence: 91%

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

2012

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Nucleic acid biosensors have a growing number of applications in genetics and biomedicine. This contribution is a critical review of the current state of the art concerning the use of nucleic acid analogues, in particular peptide nucleic acids (PNA) and locked nucleic acids (LNA), for the development of high-performance affinity biosensors. Both PNA and LNA have outstanding affinity for natural nucleic acids, and the destabilizing effect of base mismatches in PNA- or LNA-containing heterodimers is much higher than in double-stranded DNA or RNA. Therefore, PNA- and LNA-based biosensors have unprecedented sensitivity and specificity, with special applicability in DNA genotyping. Herein, the most relevant PNA- and LNA-based biosensors are presented, and their advantages and their current limitations are discussed. Some of the reviewed technology, while promising, still needs to bridge the gap between experimental status and the harder reality of biotechnological or biomedical applications.

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Section: Electrochemicalmentioning

confidence: 91%

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

2012

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“…Most other electrochemical biosensors for virus detection described in literature used synthetic DNA oligonucleotides as model analytes instead of RNA or real samples (e.g., Ahour et al 2013;Pournaghi-Azar et al 2010;Rai et al 2012). The applicability of these sensor principles with real samples has still to be demonstrated.…”

Section: Electrochemical Biosensors For Rna-based Detection Of Virusesmentioning

confidence: 99%

Electrochemical Detection of RNA

Pöhlmann

Sprinzl

2015

RNA Technologies

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“…8). The limit of detection was determined by using the equation y LOD (I)=y B +3S y/x , where S y/x standard deviation of blank (in this study, standard deviation of the calibration graph) and y B is signal of the blank (in this study intercept of calibration graph) [39]. Also, regression equation was y(I)=3.02+7.51c (μM).…”

Section: Diagnostic Performance Of the Sensormentioning

confidence: 99%

A Nucleic Acid Biosensor for Detection of Hepatitis C Virus Genotype 1a Using Poly(l-Glutamic Acid)-Modified Electrode

Dönmez

Arslan

2015

Appl Biochem Biotechnol

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An electrochemical nucleic acid biosensor based on label-free DNA detection method was prepared for the first time by using electropolymerized poly(L-glutamic acid)-modified pencil graphite electrode (PGA/PGE) for detection of hepatitis C virus genotype 1a (HCV1a). Inosine-substituted 20-mer probes related to the HCV1a were immobilized onto PGA/PGE surface by covalent linking with the formation of amide bonds. Square wave voltammetry (SWV) was used to monitor the oxidation signal of guanine in the hybridization events, which gave an oxidation peak at +1.05 V. An increase in the oxidation signal of guanine was showed by hybridization of the probe with the complementary DNA. Noncomplementary oligonucleotides were also used to investigate the selectivity of the biosensor. The proposed nucleic acid biosensor was linear in the range of 50 nM to 1.0 μM, exhibiting a limit of detection of 40.6 nM. Finally, single-stranded synthetic PCR product analogues of HCV1a were performed in optimal condition. This PGA-modified nucleic acid sensor is cost-effective and disposable, and besides, it has superior electrocatalytic effect on the oxidation of guanine.

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Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization

Cited by 43 publications

References 39 publications

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

Electrochemical Detection of RNA

A Nucleic Acid Biosensor for Detection of Hepatitis C Virus Genotype 1a Using Poly(l-Glutamic Acid)-Modified Electrode

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