Electrochemical Detection of RNA

Pöhlmann, Christopher; Sprinzl, Mathias

doi:10.1007/978-3-319-17305-4_2

Cited by 4 publications

(2 citation statements)

References 99 publications

(98 reference statements)

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“…136 Electrochemical detection of miRNAs is generally read via voltametric, amperometric, and impedimetric approaches. 137,138 One example of such strategies is the direct oxidation based analysis of circulating miRNA bases as demonstrated by Lusi et al 139 In this method, miR-122 was hybridized with its inosine substitute capture probe. Carbon based nanostructured electrode and electroactive polymers were used to increase the electroactive area and reduce the electrical resistance on the electrode.…”

Section: Nanopore-based Sensorsmentioning

confidence: 99%

MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

Aziz

Mahmudunnabi

Umer

et al. 2020

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Section: Nanopore-based Sensorsmentioning

confidence: 99%

MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

Aziz

Mahmudunnabi

Umer

et al. 2020

Analyst

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“…To avoid the challenges of enzymatic amplification of mRNA, several amplificationfree assays coupled with optical and electrochemical readouts have been developed (Carrascosa et al, 2016;Pöhlmann and Sprinzl, 2015). Among them, electrochemical methods have shown great promise towards mRNA detection due to their high sensitivity, simplicity, cost-effectiveness and compatibility with miniaturization (Mukumoto et al, 2007;Mao et al, 2008;Sato et al, 2001;Vasilyeva et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A PCR-free electrochemical method for messenger RNA detection in cancer tissue samples

Islam

Gopalan

Haque

et al. 2017

Biosensors and Bioelectronics

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Despite having reliable and excellent diagnostic performances, the currently available messenger RNA (mRNA) detection methods mostly use enzymatic amplification steps of the target mRNA which is generally affected by the sample manipulations, amplification bias and longer assay time. This paper reports an amplification-free electrochemical approach for the sensitive and selective detection of mRNA using a screen-printed gold electrode (SPE-Au). The target mRNA is selectively isolated by magnetic separation and adsorbed directly onto an unmodified SPE-Au. The surface-attached mRNA is then measured by differential pulse voltammetry (DPV) in the presence of [Fe(CN)] redox system. This method circumvents the PCR amplification steps as well as simplifies the assay construction by avoiding multiple steps involved in conventional biosensing approaches of using recognition and transduction layers. Our method has demonstrated good sensitivity (LOD = 1.0pM) and reproducibility (% RSD = <5%, for n = 3) for detecting FAM134B mRNA in two cancer cell lines and a small cohort of clinical samples (number of samples = 26) collected from patients with oesophageal cancer. The analytical performance of our method is validated with a standard qRT-PCR analysis. We believe that our PCR-free approach holds a great promise for the analysis of tumor-specific mRNA in clinical samples.

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Preparation of Cobalt Nanoparticles

et al. 2021

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The development of modern chemistry is currently proceeding in several priority areas including investigations focused on the synthesis, stabilisation, and application of transition metal nanoparticles (NPs), which are widely used in physical, chemical, engineering, and biomedical processes. A special place among known transition metal NPs is occupied by cobalt nanoparticles, since they are used for highly important targets, such as creation of new catalysts, magnetic devices, composites, or carriers for drug delivery. The selective preparation of NPs is a difficult task that requires special conditions and has some limitations. In this minireview, we summarise the most successful and most efficient methods for obtaining Co NPs, including chemical and physical aspects of their preparation.

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Electrochemical Detection of RNA

Cited by 4 publications

References 99 publications

MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

A PCR-free electrochemical method for messenger RNA detection in cancer tissue samples

Preparation of Cobalt Nanoparticles

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