MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

Aziz, Nahian Binte; Mahmudunnabi, Rabbee G.; Umer, Muhammad; Sharma, Shayna; Rashid, Md Abdur; Alhamhoom, Yahya; Shim, Yoon‐Bo; Salomón, Carlos; Shiddiky, Muhammad J. A.

doi:10.1039/c9an02263e

Cited by 45 publications

(23 citation statements)

References 151 publications

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“…The IPA analysis identified several members of the micro-RNAs as potential upstream regulators of the cathine and cathinone. These micro-RNAs have previously been reported to have different important roles in ovarian cancer regulation, prognosis, and/or diagnosis ( Alshamrani, 2020 ; Aziz et al, 2020 ; Ferreira et al, 2020 ). Hence, our present analysis could provide potential directions for future studies on the further elucidation of these micro-RNAs’ regulations of the cellular and molecular events induced by khat.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Catha edulis Extract Effects on the Cellular and Molecular Signaling in SKOV3 Cells

et al. 2021

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Khat (Catha edulis (Vahl) Endl.) is an evergreen flowering shrub used as a stimulant in many regions worldwide including East Africa, the Arabian Peninsula, Europe, and the United States. Chewing leaves of khat induces excitement and euphoria, which are primarily attributed to two major constituents, cathinone and cathine. Khat also contains other important constituents such as cathedulins. A considerable number of studies reported side effects induced by the khat extracts to both embryos and adults. These include teratogenicity and developmental retardation, oral cancer and ulcers, high blood pressure, and myocardial infarction. So far, little attention has been paid to the effects of khat extracts on the molecular signaling interactions. We aimed in this study to investigate this through evaluating the effects of khat extracts on SKOV3, a human ovarian adenocarcinoma cell line. We show, by in vitro assays, that khat induces several cellular defects including reduced cell size, cell membrane damage, and apoptosis. At high khat extract concentrations, the cell metabolic activity, cell cycle, and cellular proliferation were affected. RT-qPCR analysis showed an increase in the gene expression of the apoptotic marker BAX, the tumor suppressor p53, and the inflammatory cytokine IL-6. Protein expression analysis by immunostaining showed downregulation of β-catenin, E-cadherin, and Ki-67 and upregulation of FZD8 and SPRY2, suggesting that Wnt and FGF signaling were implicated. SwissTargetPrediction in silico analysis showed that khat constituents cathine, cathinone, catheduline K2, and catheduline E5 bind to family A G-protein-coupled receptor, cause many neurological diseases and disorders such as Alzheimer's, schizophrenia, depression, and anxiety, and induce many ovarian cancer-related diseases. The analysis also showed that important signaling pathways such as CREB, Wnt, FGF, IL-6, and ERK/MAPK, and that of the endometrial cancer, and cell cycle were implicated. Upstream regulators of cathine and cathinone were found to potentially target several molecules including interleukin-8, MMP2, PLAU, and micro-RNAs. In conclusion, khat induces significant cellular and molecular changes that could potentially cause a wide range of serious diseases and syndromes. Such an impact could have a heavy burden on the health care system in the countries where khat is consumed.

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Section: Discussionmentioning

confidence: 99%

Unraveling the Catha edulis Extract Effects on the Cellular and Molecular Signaling in SKOV3 Cells

et al. 2021

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“…Ovarian cancer. Ovarian cancer is the seventh most common type of malignant neoplasm in women and the eighth cause of mortality (102)(103)(104). Most patients with ovarian cancer are typically diagnosed at an advanced stage of the disease (105).…”

Section: Potential Role Of Art In Human Malignanciesmentioning

confidence: 99%

Progress on the study of the anticancer effects of artesunate (Review)

et al. 2021

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Artesunate (ART) is a derivative of artemisinin that is extracted from the wormwood plant Artemisia annua. ART is an antimalarial drug that has been shown to be safe and effective for clinical use. In addition to its antimalarial properties, ART has been attracting attention over recent years due to its reported inhibitory effects on cancer cell proliferation, invasion and migration. Therefore, ART has a wider range of potential clinical applications than first hypothesized. The aim of the present review was to summarize the latest research progress on the possible anticancer effects of ART, in order to lay a theoretical foundation for the further development of ART as a therapeutic option for cancer. Contents1. Introduction 2. Source and activity of ART 3. Anticarcinogenic mechanism of ART 4. Potential role of ART in human malignancies 5. Summary and perspectives

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“…These approaches are based on the RNA biosensors with (i) an electroactive labeled DNA probe sequence, (ii) the use of a catalyst For this reason, the development of other more efficient and less expensive emerging techniques is important and vital for cancer diagnosis and therapy. A variety of methods providing high sensitivity and specificity, and that are easy to handle have been recently developed based on various direct detection methods such as photoelectrochemical, localized surface plasmon resonance, and electrochemical biosensors [33].…”

Section: Brainmentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

Abstract: Ovarian cancer is the most aggressive of all gynaecological malignancies and is the leading cause of cancer-associated mortality worldwide.

Cited by 45 publications

References 151 publications

Unraveling the Catha edulis Extract Effects on the Cellular and Molecular Signaling in SKOV3 Cells

Unraveling the Catha edulis Extract Effects on the Cellular and Molecular Signaling in SKOV3 Cells

Progress on the study of the anticancer effects of artesunate (Review)

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

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