Direct comparison of selected methods for genetic categorisation of Cryptosporidium parvum and Cryptosporidium hominis species

Chalmers, Rachel M.; Ferguson, Christobel; Cacciò, Simone M.; Gasser, Robin B.; EL-Osta, Youssef G. Abs; Heijnen, Leo; Xiao, Lihua; Elwin, Kristin; Hadfield, Stephen J.; Sinclair, Martha Irvine; Stevens, Melita

doi:10.1016/j.ijpara.2005.01.001

Cited by 134 publications

(135 citation statements)

References 45 publications

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“…The typing methods used in this study enabled investigation of a vast number of specimens with very little loss in resolution [22]. Potential mis-identifications in the COWP assay that are currently known include the Cryptosporidium rabbit genotype confounding for C. hominis [6] and the mouse genotype mistaken for C. parvum [36].…”

Section: Discussionmentioning

confidence: 99%

Long-term Cryptosporidium typing reveals the aetiology and species-specific epidemiology of human cryptosporidiosis in England and Wales, 2000 to 2003

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Long-term Cryptosporidium typing reveals the aetiology and species-specific epidemiology of human cryptosporidiosis in England and Wales, 2000 to 2003

et al. 2009

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“…Because their sequences had higher intraspecific variation than the ribosomal RNA gene regions (Morgan et al, 1999b), it was suggested that other Cryptosporidium genes targets should be used for amplification including the Cryptosporidium oocyst wall protein (COWP), 16S rRNA, Hsp70, Actin, β-Tubulin, gp60, microsatellites, minisatellites and extrachromosomal double-stranded RNA (Xiao et al, 2004;Caccio et al, 2005;Coklin et al, 2007). As well as the Internal Transcribed Spacers (ITS) of ribosomal DNA were useful for the detection of genetic variability within species (Chalmers et al, 2005;Schindler et al, 2005). Synchronized analysis of the obtained morphological simultaneous with molecular criteria of Cryptosporidium buffalo's oocytes in the present study could prove that the isolates were C. parvum.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abdelrahma¹,

Megeed²,

Hammam³

et al. 2015

Research J. of Parasitology

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Cryptosporidium an apicomplexan parasite has the ability to induce diarrhea in bovines, goats, pigs, dogs and cats worldwide. In this study, buffalo calves fecal samples were examined after staining their smears with Modified Ziehl-Neelsen Stain (MZN). Ileal sections were examined for the detection of pathological changes. Further molecular characterization was done using nested PCR amplification and partial sequence analysis. The detected oocysts were morphologically similar to Cryptosporidium parvum. Light microscopic examination of Cryptosporidium infected ileal Tissue Section (TS) stained with H and E revealed the presence of altered mucosal architecture with congestion of blood vessels, infiltration, sloughing and complete erosion of epithelial cells and shortening, blunting, stunting and atrophy of the intestinal villi. Molecular characterization gave PCR amplicons of 18S SSU rRNA gene products approximately at 823 bp. Sequences proved specified generalized relatedness with 21 species of Cryptosporidium but the nucleotide homogeneity percentage was insufficient to designate species or genotypes. Further bioinformatics analysis showed that resulting Cryptosporidium isolates had the closest match with three isolates. It was implied that the Cryptosporidium isolates is mostly like Cryptosporidium parvum (JX237832.1) previously isolated from buffaloes in Ismailia province.

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“…The IIaA15G2R1 subtype was reported in three lambs linked to a human infection in the UK (Chalmers et al 2005). Recently, sheep have also been implicated as a potential source for human C. ubiquitum infections in the UK by gp60 subtyping .…”

Section: R Y P T O S P O R I D I U M S P E C I E S I N a N I M A L Smentioning

confidence: 99%

Cryptosporidiumspecies in humans and animals: current understanding and research needs

2014

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S U M M A R YCryptosporidium is increasingly recognized as one of the major causes of moderate to severe diarrhoea in developing countries. With treatment options limited, control relies on knowledge of the biology and transmission of the members of the genus responsible for disease. Currently, 26 species are recognized as valid on the basis of morphological, biological and molecular data. Of the nearly 20 Cryptosporidium species and genotypes that have been reported in humans, Cryptosporidium hominis and Cryptosporidium parvum are responsible for the majority of infections. Livestock, particularly cattle, are one of the most important reservoirs of zoonotic infections. Domesticated and wild animals can each be infected with several Cryptosporidium species or genotypes that have only a narrow host range and therefore have no major public health significance. Recent advances in next-generation sequencing techniques will significantly improve our understanding of the taxonomy and transmission of Cryptosporidium species, and the investigation of outbreaks and monitoring of emerging and virulent subtypes. Important research gaps remain including a lack of subtyping tools for many Cryptosporidium species of public and veterinary health importance, and poor understanding of the genetic determinants of host specificity of Cryptosporidium species and impact of climate change on the transmission of Cryptosporidium.

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Direct comparison of selected methods for genetic categorisation of Cryptosporidium parvum and Cryptosporidium hominis species

Cited by 134 publications

References 45 publications

Long-term Cryptosporidium typing reveals the aetiology and species-specific epidemiology of human cryptosporidiosis in England and Wales, 2000 to 2003

Long-term Cryptosporidium typing reveals the aetiology and species-specific epidemiology of human cryptosporidiosis in England and Wales, 2000 to 2003

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Cryptosporidiumspecies in humans and animals: current understanding and research needs

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