Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays

Treml, Jakub; Večeřová, Petra; Herczogová, Petra; Šmejkal, Karel

doi:10.3390/molecules26092534

Cited by 17 publications

(14 citation statements)

References 60 publications

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“…The advantage of CAA assays over ORAC and DPPH assays is the involvement of the cellular environment in the activity [ 48 ]; however, our results in the CAA assay did not confirm the promising activity of the P. flabellatus 5013 extract. None of the tested extracts had significantly higher activity than the quercetin control.…”

Section: Resultscontrasting

confidence: 61%

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

et al. 2022

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Within the group of higher fungi, edible medicinal mushrooms have a long history of being used as food and in folk medicine. These species contain biologically active substances with many potential beneficial effects on human health. The Pleurotus genus is representative of medicinal mushrooms because Pleurotus ostreatus is one of the most commonly cultivated culinary mushrooms. In our study, we focused on lesser-known species in the genus Pleurotus and measured their antioxidant and anti-inflammatory activity. We prepared extracts of the mushrooms and analyzed them using HPLC−HRMS, GC−MS, and 1H-NMR. Significant differences in biological activities were found among the Pleurotus spp. extracts. A MeOH extract of P. flabellatus was the most active as a radical scavenger with the highest ORAC, while a chloroform extract had significant anti-inflammatory COX-2 activity. The 80% MeOH extract of P. flabellatus contained the highest amounts of ergosterol, ergothioneine, and mannitol. The 80% MeOH extract of P. ostreatus Florida was the most active in the NF-κB inhibition assay and had the highest content of β-glucans (43.3% by dry weight). Given the antioxidant and anti-inflammatory properties of P. flabellatus, the potential therapeutic usefulness of this species is worth evaluating through in-depth investigations and confirmation by clinical trials.

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Section: Resultscontrasting

confidence: 61%

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

et al. 2022

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“…The former can be evaluated by the free radical scavenging assays, and the later can be determined by the expression of anti-oxidant enzymes. Interestingly, one study reports that AC shows no effects on the expression of anti-oxidant enzymes but the direct scavenging activity [56] .…”

Section: Anti-oxidative Stressmentioning

confidence: 99%

The pharmacokinetic property and pharmacological activity of acteoside: A review

Xiao¹,

Ren²,

Wu³

2022

Biomedicine & Pharmacotherapy

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“…The cells are then lysed, and the protein concentration determined according to the Bradford method. Then, according to the instructions, measure CAT activity in cell lysates using the catalase assay kit [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Wen

et al. 2022

Nutrients

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Many researchers have found that Pb exposure can cause oxidative stress damage to the body’s tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidative stress indicators, such as ROS, GSH/GSSG, MDA, SOD, CAT, GPx, and GR, were tested with biochemical kit. Protein expression of Keap1, Nrf2, and TXNIP was measured by Western blot. Compared with the control group, Pb reduced the cell viability of PC12 cells. However, BSP treatment significantly increased the viability of PC12 cells induced by lead exposure (p < 0.05). Lead can enrich the contents of MDA and ROS, but decrease the amount of CAT, SOD, GR, GPx, and GSH/GSSG in PC12 cells, while BSP can alleviate it (p < 0.05). Lead can enhance the expression of Keap1 and TXNIP proteins, but reduce Nrf2 expression. In contrast, BSPs reversed this phenomenon (p < 0.05). BSPs can alleviate oxidative stress injury induced by lead in PC12 cells through the Keap1/Nrf2/TXNIP signaling pathway.

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Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays

Cited by 17 publications

References 60 publications

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

The pharmacokinetic property and pharmacological activity of acteoside: A review

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

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