Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Abstract

Many researchers have found that Pb exposure can cause oxidative stress damage to the body’s tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidative stress indicators, such as ROS, GSH/GSSG, MDA, SOD, CAT, GPx, and GR, were tested with biochemical kit. Protein expression of Keap1, Nrf2, and TXNIP was measured by Western blot. Compared with the control group, Pb reduced the cell viability of PC12 cells. However, BSP treatment significantly increased the viability of PC12 cells induced by lead exposure (p < 0.05). Lead can enrich the contents of MDA and ROS, but decrease the amount of CAT, SOD, GR, GPx, and GSH/GSSG in PC12 cells, while BSP can alleviate it (p < 0.05). Lead can enhance the expression of Keap1 and TXNIP proteins, but reduce Nrf2 expression. In contrast, BSPs reversed this phenomenon (p < 0.05). BSPs can alleviate oxidative stress injury induced by lead in PC12 cells through the Keap1/Nrf2/TXNIP signaling pathway.

Effects of folic acid on oxidative damage of kidney in lead-exposed rats

“…It has been reported that hydrogen peroxide-induced oxidative stress in cells causes an increase in the levels of ROS and MDA and a decrease in SOD and GSH ( 42 ). Vitamin C in combination with vitamin E has been reported to alleviate oxidative stress caused by lead exposure by increasing the levels of SOD, CAT, and GSH-Px ( 43 , 44 ). The present study found that lead exposure significantly increased MDA levels and significantly decreased SOD, GSH, and GSH-Px simultaneously.…”

A systematic review of black soybean (Glycine max (L.) Merr.): Nutritional composition, bioactive compounds, health benefits, and processing to application

“…Crude polysaccharides were extracted from BS exhibited antioxidant effects, although the DPPH and superoxide anion free radicals of crude polysaccharides (72.22% and 77.88%, respectively) were slightly lower than those of the V C control (97.91% and 97.56%, respectively; J. Liu, Wen, Kan, Zhang, et al., 2015). Many studies have claimed that BS peptides exhibit antioxidant effects by activating the Keap1/Nrf2 signaling pathway (Cui et al., 2022; N. Li et al., 2022; J. Ren et al., 2021; Y. H. Wu et al., 2014). Particularly, Y. H. Wu et al.…”

Antioxidant Capacity and Protective Effects on H2O2-Induced Oxidative Damage in PC12 Cells of the Active Fraction of Brassica rapa L.

“…Typically, the same analysis used to detect cell viability over a specified period can reflect cell proliferation. Previous research revealed that the PC12 cell, a rat pheochromocytoma cell line, which has been widely used as a cell model in studies of neurodegenerative diseases for the PC12 cell, has similar neuronal characteristics, physiology, and pathology of the nerve cells [38]. PC12 cells (1 × 10 5 cells/mL) were treated with different concentrations (0-800 µg/mL) of BR fractions for 24 h at 37 • C after normal culture for 24 h to observe toxicity.…”