Dimorphism in Mucor Species

Ruíz-Herrera, José

doi:10.1007/978-1-4615-2834-0_21

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Dimorphism in certain Mrcor species is well documented and the transition to a yeast-like morphology, i.e. spherical multipolar budding cells, is induced by microaerobiosis or by microaerobiosis in combination with a high CO, level or glucose concentration (Bartnicki-Garcia, 1963, 1968Bartnicki-Garcia and Xckerson, 1962;Ruiz-Herrera, 1993).…”

Section: Resultsmentioning

confidence: 99%

Production of gamma-linolenic acid by Mucor circinelloides and Mucor rouxii with acetic acid as carbon substrate

et al. 1995

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University of the Ormgc Free St&-, PO. 80-v 339, 9300 l~lorn~fontein, South .4frica SUMMARYThe production of gamma-linolenic acid (GLA) by h41rcor circimlloides CBS 203.28 and MI rot/xii CBS 416.77 in fed-batch cultures operated in pH-stat mode with acetic acid as carbon substrate and titrant compared favourably with the performance of A4. circille//oides in batch culture on glucose. On acetic acid A4. circimlloides accumulated up to 39.8 mg GLA/g biomass, with a crude oil content of 28% containing 91% neutral lipids. The GLA content of the neutral lipid fraction was 15.6%.

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Section: Resultsmentioning

confidence: 99%

Production of gamma-linolenic acid by Mucor circinelloides and Mucor rouxii with acetic acid as carbon substrate

et al. 1995

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“…These conditions change the redox potential of the cell where a sensor unchains the morphogenetic response. In Mucor species where microaerobiosis is not sufficient to induce yeast-like growth, it is suggested that the sensor has such a low potential that either additional catabolite repression or an unknown alteration brought about by a high CO 2 tension is necessary to initiate the transition (Ruiz-Herrera 1993). It is known that under low levels of glucose or CO 2 , or both, hyphal development occurred; at high levels, occurs the eliciting yeast development of Mucor sp.…”

Section: Introductionmentioning

confidence: 99%

Terpenoid biotransformations by Mucor species

et al. 2013

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Terpenoids are natural products of great interest due to their widespread use in agrochemicals, drugs, fragrances, flavouring and pigments. Biocatalysts are increasingly being used in the search for new derivatives with improved properties especially to obtain structurally novel leads for new drugs which are difficult to obtain using conventional organic chemical methods. This review, covering up to the end of 2012, reports on the application of Mucor species as catalysts in terpenoid biotransformation to obtain new drug targets, enhance pharmacological activity or decrease the unwanted effects of starting material.

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“…The ®lamentous members of the genus Mucor have been the focus of numerous studies in both basic and applied areas of biological research, such as the dimorphic transition (Orlowsky 1991;Ruiz Herrera 1993), the production of extracellular enzymes (Outtrup and Boyce 1990;Godtfredsen 1990), or the role sex pheromones play in the Mucorales (Gooday 1994). Recently, attention has been focused on the biosynthesis of carotenoids in Mucor circinelloides, and mutants altered in this pathway have been isolated and analysed (Navarro et al 1995;Ruiz-Hidalgo et al 1995;Fraser et al 1996;Velayos et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Interallelic complementation at the pyrF locus and the homodimeric nature of orotate phosphoribosyltransferase (OPRTase) in Mucor circinelloides

Velayos¹,

Alvarez²,

Eslava³

et al. 1998

Mol Gen Genet

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Using 5-fluoroorotic acid (5-FOA) as a positive selection system we isolated mutants of Mucor circinelloides altered in the pyrimidine biosynthetic pathway. These mutants were found to be deficient either in orotidine-5'-monophosphate decarboxylase (OMPdecase), or in orotate phosphoribosyltransferase (OPRTase) activity. Complementation tests among mutants lacking OPRTase activity classified them into three groups, thus suggesting the possibility of interallelic complementation. To investigate this hypothesis a cDNA clone corresponding to the OPRTase-encoding gene of M. circinelloides was isolated by direct complementation of E. coli. The genomic copy transformed to prototrophy one member of each of the three classes of OPRTase-deficient mutants. We therefore concluded that they were all altered at the same locus, the pyrF locus. The corresponding alleles were cloned and sequenced. Comparisons of the amino acid sequence of M. circinelloides OPRTase with those of E. coli and S. typhimurium revealed a high degree of similarity in secondary and tertiary structure. As the two bacterial enzymes exist as dimers, a homodimeric quaternary structure of the M. circinelloides mature protein can be assumed. This would also explain the interallelic complementation between some pyrF mutants. The mutations found could affect either the active site or the structure of the dimer interface of the OPRTase.

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Dimorphism in Mucor Species

Cited by 6 publications

References 21 publications

Production of gamma-linolenic acid by Mucor circinelloides and Mucor rouxii with acetic acid as carbon substrate

Production of gamma-linolenic acid by Mucor circinelloides and Mucor rouxii with acetic acid as carbon substrate

Terpenoid biotransformations by Mucor species

Interallelic complementation at the pyrF locus and the homodimeric nature of orotate phosphoribosyltransferase (OPRTase) in Mucor circinelloides

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