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University of the Ormgc Free St&-, PO. 80-v 339, 9300 l~lorn~fontein, South .4frica SUMMARYThe production of gamma-linolenic acid (GLA) by h41rcor circimlloides CBS 203.28 and MI rot/xii CBS 416.77 in fed-batch cultures operated in pH-stat mode with acetic acid as carbon substrate and titrant compared favourably with the performance of A4. circille//oides in batch culture on glucose. On acetic acid A4. circimlloides accumulated up to 39.8 mg GLA/g biomass, with a crude oil content of 28% containing 91% neutral lipids. The GLA content of the neutral lipid fraction was 15.6%.

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