Diminished expression of CD19 in B‐cell lymphomas

Yang, Wei; Agrawal, Neeta N.; Patel, Jigar; Edinger, Alison; Osei, Ebenezer; Thut, Dawn M.; Powers, Jennifer; Meyerson, Howard

doi:10.1002/cyto.b.20030

Cited by 58 publications

(27 citation statements)

References 33 publications

(41 reference statements)

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“…We have demonstrated that evaluating the intensity of staining for CD19 can help in this differential diagnosis. It has been previously shown that decreased CD19 antigen expression is seen more frequently in follicular lymphoma than in other lymphoma subtypes (16,21). Our results confirm this finding and specifically demonstrate that there is a significantly lower MCF value for CD19 in FL cases as compared to MZL cases.…”

Section: Discussionsupporting

confidence: 90%

Marginal zone B‐cell lymphoma: A retrospective immunophenotypic analysis

Kost

Holden

Mann

2008

Cytometry Part B Clinical

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Background: Marginal zone B-cell lymphoma (MZL) comprises three related yet biologically distinct subtypes-splenic MZL (SMZL), nodal MZL (NMZL), and extranodal MZL of MALT type (MALT). In cases without adequate morphology, immunophenotypic characterization by flow cytometric immunophenotyping (FCI) relies heavily on exclusion of other low-grade lymphomas. We performed a retrospective review of FCI studies of MZL to search for immunophenotypes specific for MZL and its subtypes. We compared these to follicular lymphoma (FL) as we were specifically interested in differentiating MZL from CD10 negative FL.Design: FCI findings for MZL and FL cases were reviewed. Statistical analysis of patterns and intensity of antigen expression [mean channel fluorescence (MCF)] were performed.Results: Thirty-one cases of MZL (7 SMZL, 6 NMZL, 15 MALT, 3 MZL not otherwise specified) and 31 cases of FL were identified. All expressed CD19, CD20, and CD45. Thirty-two percent of MZL and 77% of FL expressed CD38. Expression of CD11c was seen in 57% of SMZL and 8% of other MZL (P < 0.01). Statistically significant differences in antigen expression between MZL and FL were seen for CD10, CD11c, and CD38. CD19 expression was significantly brighter in MZL (mean MCF of 455.3) than in FL (mean MCF of 166.9) (P < 0.001). MCF for isotype controls and CD20 were similar for FL and MZL.Conclusions: MZL expresses typical pan-B-cell antigens. Expression of CD11c is highly associated with SMZL. Levels of CD19 expression in conjunction with CD11c and CD38 expression can distinguish MZL from CD10 negative FL. q

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Section: Discussionsupporting

confidence: 90%

Marginal zone B‐cell lymphoma: A retrospective immunophenotypic analysis

Kost

Holden

Mann

2008

Cytometry Part B Clinical

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“…The prognostic significance of CD20 expression is contentious in other lymphoma subtypes and, to our knowledge, has never been specifically examined in DLBCL. 6,[26][27][28][29] We demonstrate that patients who have reduced CD20 expression but bright CD19 expression (discordant CD20) on their biopsies taken at diagnosis have a markedly inferior OS after treatment with CHOP with or without rituximab, independent of the IPI.…”

Section: Discussionmentioning

confidence: 72%

Diffuse large B-cell lymphoma: reduced CD20 expression is associated with an inferior survival

Johnson

Boyle

Bashashati

et al. 2009

Blood

130

113

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“…While CD19 is thought to be a very useful lineage defining antigen for B cells, decreased or absent CD19 expression can be seen in B cell malignancies (1). Decreased or absent CD19 is more common in follicular lymphoma or DLBCL, but aberrant decrease or loss can occur in a variety of B lineage neoplasms (1,2). CD20 is also typically seen in mature B cells, though decreased CD20 expression is a classic immunophenotypic aberrancy of the neoplastic B cells comprising a CLL/SLL clone (3).…”

Section: Discussionmentioning

confidence: 99%

Case study interpretation—New Orleans: Case 4

Linden

2013

Cytometry Part B Clinical

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Diminished expression of CD19 in B‐cell lymphomas

Cited by 58 publications

References 33 publications

Marginal zone B‐cell lymphoma: A retrospective immunophenotypic analysis

Marginal zone B‐cell lymphoma: A retrospective immunophenotypic analysis

Diffuse large B-cell lymphoma: reduced CD20 expression is associated with an inferior survival

Case study interpretation—New Orleans: Case 4

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