Marginal zone B‐cell lymphoma: A retrospective immunophenotypic analysis

Abstract

Background: Marginal zone B-cell lymphoma (MZL) comprises three related yet biologically distinct subtypes-splenic MZL (SMZL), nodal MZL (NMZL), and extranodal MZL of MALT type (MALT). In cases without adequate morphology, immunophenotypic characterization by flow cytometric immunophenotyping (FCI) relies heavily on exclusion of other low-grade lymphomas. We performed a retrospective review of FCI studies of MZL to search for immunophenotypes specific for MZL and its subtypes. We compared these to follicular lymphoma (FL) as we were specifically interested in differentiating MZL from CD10 negative FL.Design: FCI findings for MZL and FL cases were reviewed. Statistical analysis of patterns and intensity of antigen expression [mean channel fluorescence (MCF)] were performed.Results: Thirty-one cases of MZL (7 SMZL, 6 NMZL, 15 MALT, 3 MZL not otherwise specified) and 31 cases of FL were identified. All expressed CD19, CD20, and CD45. Thirty-two percent of MZL and 77% of FL expressed CD38. Expression of CD11c was seen in 57% of SMZL and 8% of other MZL (P < 0.01). Statistically significant differences in antigen expression between MZL and FL were seen for CD10, CD11c, and CD38. CD19 expression was significantly brighter in MZL (mean MCF of 455.3) than in FL (mean MCF of 166.9) (P < 0.001). MCF for isotype controls and CD20 were similar for FL and MZL.Conclusions: MZL expresses typical pan-B-cell antigens. Expression of CD11c is highly associated with SMZL. Levels of CD19 expression in conjunction with CD11c and CD38 expression can distinguish MZL from CD10 negative FL. q

Tissue flow cytometry immunophenotyping in the diagnosis and classification of non‐Hodgkin's lymphomas: A retrospective evaluation of 1,792 cases

“…However, only few reports are available regarding the role of FC in tissue diagnosis of lymphoma (25)(26)(27)(28)(29)(30)(31), and literature mainly reports tissue FC studies in selected lymphoma subtypes (12,14,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48). In the present study, a high concordance between morphology and an abnormal immunophenotypic pattern was reported on hematological malignancies detected by FC (1,268/1,304, 97.2%, including composite pathologies such as composite lymphomas and lymphoma plus carcinoma) with the exception of HL.…”

Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray

“…Ранее было показано, что маркер CD11c всегда присутствует на опухолевых клетках при ВКЛ и ВКЛ-В [3,5], в большинстве случаев (до 50 %) -при ЛКМЗС [12,19] и изредка экспрессируется на поверхности опухолевых клеток при В-ХЛЛ и лимфоме из клеток мантийной зоны [26]. В нашей работе у 36 % пациентов с ЛКМЗС опухолевые клет-ки экспрессировали CD11с.…”

“…Хотя все источники указывают на морфологическую разнородность опу-холевых клеток при ЛКМЗС, подробное описание Частота выявления иммунофенотипов опухолевых лимфоцитов при лимфоме из клеток маргинальной зоны селезенки по данным собственного исследования и литературных публикаций [5,11,12,19,20] Frequency of identification of tumor lymphocyte immunophenotypes from splenic marginal zone lymphoma according to our data and literature [5,11,12,19,20] Фундаментальные исследования в практической медицине на современном этапе возможных морфологических типов клеток при дан-ном заболевании в литературе не встречается, что мо-жет затруднить диа гностику ЛКМЗС [30]. Наиболее полное сравнительное описание морфологии и имму-нофенотипа циркулирующих опухолевых клеток при ЛКМЗС, ВКЛ и ВКЛ-В содержится в работе H. Shao и соавт.…”

Marginal Zone Lymphomas: Management of Nodal, Splenic, and MALT NHL

“…This phenotype is similar to MALT lymphoma; however, an occasional case will express surface IgD, which is seen in SMZL. 3,4 In regards to the cell of origin, NMZL may arise from different types of B cells found in the marginal zone. 5 Beyond morphologic and immunophenotypic descriptions, the biology of NMZL is not well studied.…”