Dilute-and-shoot coupled to nanoflow liquid chromatography high resolution mass spectrometry for the determination of drugs of abuse and sport drugs in human urine

El Dopaje Deportivo Como Cuestión Socialmente Viva: Una Revisión Bibliográfica

“…10 Algunos estudios especifican los métodos de detección de estas sustancias prohibidas, [96][97][98][99][100][101][102][103][104][105][106][107][108] al igual que los perfiles de los atletas que recurren al dopaje. [109][110][111] • La categoría 4. Bioquímica-Fisiología incluye 16 textos que estudian las causas, consecuencias e implicaciones a nivel atómico-molecular, microscópico y macroscópico, del consumo de sustancias prohibidas en la morfofisiología de los atletas.…”

Hyphenated high-resolution mass spectrometry—the “all-in-one” device in analytical toxicology?

“…Besides these general approaches, various methods were published for particular drug groups e.g., hallucinogenic phenethylamines (non-targeted) [33], low-dosed opioids (non-targeted data acquisition coupled with targeted data processing) [34], or synthetic cannabinoids (non-targeted) [35]. Thanks to its high sensitivity, LC-HRMS/MS was successfully applied also for broad-spectrum drug screening in low sample volumes such as dried blood spots [36] or in samples with low concentrations such as hair samples [37], urine after dilute-and-shoot application [38], or wastewater [39]. Finally, Mollerup et al [40] described a new approach with LC-ion mobility-HRMS/MS for broad scope screening based on prediction of collision cross section and retention time with machine learning using artificial neural networks.…”

Annual banned‐substance review: Analytical approaches in human sports drug testing

“…The applicability of using nanoLC–HRMS to the direct analysis of diluted urine was discussed by Alcaranta‐Duran et al Following a 1:50 (v/v) dilution of urine, specimens were injected onto a nanoLC analytical column (150 x 0.075 mm, particle size 3 μm), and analytes were gradient‐eluted using water (solvent A) and methanol (solvent B) both containing 0.1% formic acid. The mass spectrometer was operated in positive ionization mode with full scan (m/z 170–1000) and all‐ion fragmentation settings, employing a resolution of 70 000 and 15 000, respectively.…”

“…In this review, literature published between October 2017 and September 2018 has been evaluated (Table ), which accounts for new and complementary sports drug testing approaches advanced by enhanced analytical instrumentation and optimized target analyte selection. In addition, next‐generation multi‐analyte test methods are featured, which have continued to represent preferred options for initial sample testing. Data obtained from confiscated products have necessitated increased vigilance concerning modified doping agents arguably designed to support the evasion of doped athletes …”

“…Beta-blockers all analytes of interest. Besides the subset of AAS, which were detected at limits of detection (LODs) sufficiently below respective MRPLs, also sulfoconjugated metabolites of testosterone (T), epitestosterone (E), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), androsterone (A), and etiocholanolone (ETIO) were determined in the same analytical run, thus enabling the supplementation of GC-MS-based steroid profile measurements.The applicability of using nanoLC-HRMS to the direct analysis of diluted urine was discussed by Alcaranta-Duran et al37 Following a 1:50 (v/v) dilution of urine, specimens were injected onto a nanoLC analytical column (150 x 0.075 mm, particle size 3 μm), and analytes were gradient-eluted using water (solvent A) and meth-anol (solvent B) both containing 0.1% formic acid. The mass spectrometer was operated in positive ionization mode with full scan (m/z 170-1000) and all-ion fragmentation settings, employing a resolution of 70 000 and 15 000, respectively.…”