Dilemma and breakthrough of biodegradable poly-l-lactic acid in bone tissue repair

Chen

et al. 2022

Biomater Res

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Background Fe3O4 nanoparticles are highly desired for constructing endogenous magnetic microenvironment in scaffold to accelerate bone regeneration due to their superior magnetism. However, their random arrangement easily leads to mutual consumption of magnetic poles, thereby weakening the magnetic stimulation effect. Methods In this study, magnetic nanochains are synthesized by magnetic-field-guided interface co-assembly of Fe3O4 nanoparticles. In detail, multiple Fe3O4 nanoparticles are aligned along the direction of magnetic force lines and are connected in series to form nanochain structures under an external magnetic field. Subsequently, the nanochain structures are covered and fixed by depositing a thin layer of silica (SiO2), and consequently forming linear magnetic nanochains (Fe3O4@SiO2). The Fe3O4@SiO2 nanochains are then incorporated into poly l-lactic acid (PLLA) scaffold prepared by selective laser sintering technology. Results The results show that the Fe3O4@SiO2 nanochains with unique core–shell structure are successfully constructed. Meanwhile, the orderly assembly of nanoparticles in the Fe3O4@SiO2 nanochains enable to form magnetic energy coupling and obtain a highly magnetic micro-field. The in vitro tests indicate that the PLLA/Fe3O4@SiO2 scaffolds exhibit superior capacity in enhancing cell activity, improving osteogenesis-related gene expressions, and inducing cell mineralization compared with PLLA and PLLA/Fe3O4 scaffolds. Conclusion In short, the Fe3O4@SiO2 nanochains endow scaffolds with good magnetism and cytocompatibility, which have great potential in accelerating bone repair.

Section: Discussionmentioning

confidence: 99%

Construction of magnetic nanochains to achieve magnetic energy coupling in scaffold

Chen

et al. 2022

Biomater Res

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“…It is well known that various cells, such as mesenchymal stem cells, osteoblasts, and endothelial cells are magnetically sensitive due to the diamagnetism of cell membranes [45]. Inspired by these, researchers have applied different external magnetic elds to study the roles of magnetic stimulation in bone repair in recent years [46,47]. It was found that the external magnetic elds could induce a series of cell behaviors by regulating cell surface receptors and signaling pathways via magnetic stimulations, thereby accelerating new bone regeneration or inhibiting osteoclast resorption.…”

Section: Discussionmentioning

confidence: 99%

Construction of Magnetic Nanochains to Achieve Magnetic Energy Coupling in Scaffold

Chen

et al. 2022

Preprint

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Background: Fe3O4 nanoparticles are highly desired for constructing endogenous magnetic microenvironment in scaffold to accelerate bone regeneration due to their superior magnetism. However, their random arrangement easily leads to mutual consumption of magnetic poles, thereby weakening the magnetic stimulation effect. Methods: In this study, magnetic nanochains are synthesized by magnetic-field-guided interface co-assembly of Fe3O4 nanoparticles. In detail, multiple Fe3O4 nanoparticles are aligned along the direction of magnetic force lines and are connected in series to form nanochain structures under an external magnetic field. Subsequently, the nanochain structures are covered and fixed by depositing a thin layer of silica (SiO2), and consequently forming linear magnetic nanochains (Fe3O4@SiO2). The Fe3O4@SiO2 nanochains are then incorporated into poly l-lactic acid (PLLA) scaffold prepared by selective laser sintering technology.Results: The results show that the Fe3O4@SiO2 nanochains with unique core-shell structure are successfully constructed. Meanwhile, the orderly assembly of nanoparticles in the Fe3O4@SiO2 nanochains enable to form magnetic energy coupling and obtain a highly magnetic micro-field. The in vitro tests indicate that the PLLA/Fe3O4@SiO2 scaffolds exhibit superior capacity in enhancing cell activity, improving osteogenesis-related gene expressions, and inducing cell mineralization compared with PLLA and PLLA/Fe3O4 scaffolds. Conclusion: In short, the Fe3O4@SiO2 nanochains endow scaffolds with good magnetism and cytocompatibility, which have great potential in accelerating bone repair.

“…Following that the specimens were dried for 30 min, and a fluorescence staining assay, with a fluorescence microscope (YZF-20, Shanghai Yuanren testing equipment Co., Ltd, Shanghai, China), was carried out to evaluate the cell viability. [71][72][73][74] Live cells were stained green while the dead ones were stained red. In addition, in order to observe the cell morphology on the scaffold, the cells were fixed on the scaffold and then dehydrated.…”

Section: Cell Culturementioning

confidence: 99%

Water-responsive shape memory thermoplastic polyurethane scaffolds triggered at body temperature for bone defect repair

Wang

Peng

et al. 2022

Mater. Chem. Front.

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