Digital Technology for Processing Dried Drops of Biofluids

Мартусевич, А. К.; Kovaleva, Lida K.; Karuzin, Konstantin A.; Feofilova, Maria; Бочарин, Иван; Surovegina, Alexandra; Назаров, В. Е.; Kashirina, Anastasia

doi:10.35630/2199-885x/2022/12/2.2

Cited by 3 publications

(2 citation statements)

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“…After exposure (5 minutes), each portion was centrifuged according to the standard protocol for serum isolation. The crystallogenic activity of blood serum was studied by classical crystalloscopy [3]. The result of dehydration structuring was evaluated using a system of semi-quantitative indicators described by us earlier [3].…”

Section: Methodsmentioning

confidence: 99%

“…The crystallogenic activity of blood serum was studied by classical crystalloscopy [3]. The result of dehydration structuring was evaluated using a system of semi-quantitative indicators described by us earlier [3]. The main ones are crystallizability, the structure index, the facia destruction degree and the clearity of the marginal protein zone of the micropreparation.…”

Section: Methodsmentioning

confidence: 99%

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Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Мартусевич¹,

Fedotova²,

Степанова³

et al. 2022

ArveMED

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The aim of the study was to assess the effect of reactive oxygen species on the nature of dehydration structuring of blood plasma. The study was performed on 10 whole blood samples of practically healthy volunteers, divided into 3 equal portions. The first one (control) was not manipulated, the second was treated with an ozone-oxygen mixture (ozone concentration – 500 mcg/l), and the third - with a flow from a singlet oxygen generator “Airnergy” (power – 100%). The crystallogenic activity of blood serum was studied by classical crystalloscopy using a system of semi-quantitative indicators. It was stated that the considered reactive oxygen species (ozone and singlet oxygen intermediates) increase the crystallogenic properties of blood plasma, and this effect is more pronounced with respect to the flow from the singlet oxygen generator.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Мартусевич¹,

Fedotova²,

Степанова³

et al. 2022

ArveMED

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Effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolic state and crystallogenic properties of rat blood plasma: a preclinical experimental study

Martusevich,

Surovegina,

Kononets

et al. 2023

Kuban. nauсh. med. vestnik

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Background: The multifaceted regulatory role of nitric oxide in biological systems predetermines the high value of studying the possibilities of the external control of the compound level in organs and tissues. There are several fundamentally different ways of exogenous modulation of nitric oxide metabolism. The most promising option is the use of pharmacological donors. Dinitrosyl iron complexes (DNIC) with various ligands hold a prominent place among such donors as they are considered as a natural deposited form of nitric oxide.Objective. To study the effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolism parameters and crystallogenic activity of rat blood.Methods. A preclinical experimental randomized study was conducted on 60 sexually mature male Wistar rats weighing about 250 g. The animals were divided into 6 groups, each consisting of 10 individuals. Group 1 included intact (without any manipulations) individuals. In group 2, the rats were administered daily intraperitoneal injections of 1 ml. of 0.9% sodium chloride solution for 10 days. The rats included in the other four groups received daily intraperitoneal injections of 1 ml of dinitrosyl iron complexes with glutathione ligands in an isotonic sodium chloride solution with different agent concentrations: 0.15 mM for group 3; 0.30 mM for group 4; 0.45 mM for group 5; 0.60 mM for group 6. The final indicator of the study was the assessment of the oxidative potential and crystallogenic properties of blood under the conditions of administering various doses of glutathione-containing dinitrosyl iron complexes. The following parameters were used to assess the activity of proand antioxidant systems: lipid peroxidation intensity; the total activity of antioxidant systems, and malondialdehyde concentration. The parameters for intrinsic crystallization assessment included serum facies structural index, crystallizability, assessment of the marginal facies zone, and the destruction degree of facies elements. The obtained data calculation was performed using the software packages MS Office 2013 (Microsoft Corporation, USA) and Statistica 10 (StatSoft, USA).Results. The research established that glutathione-containing dinitrosyl iron complexes have an antioxidant effect. Moreover, the manifestation of these properties demonstrates a nonlinear dependence on their dose, with a possible optimum lying in the range of 0.3–0.45 mM. The study also revealed a tendency towards crystallogenic properties activation induced by this agent, corresponding to concentrations of 0.3 and 0.45 mM.Conclusion. The undertaken studies indicate the presence of an antioxidant effect in glutathione-containing dinitrosyl iron complexes. The manifestation of these properties demonstrates a dependence on their dose with a possible optimum varying from 0.3 to 0.45 mM. The research has established the activating effect of glutathione-containing dinitrosyl iron complex injections on the crystallogenic potential of the blood serum of healthy rats. This effect consisted in an increase in the density and complexity of crystalline elements. What is more, the maximal manifestation of this tendency (for metabolic indicators as well) corresponded to concentrations of 0.3 and 0.45 mM.

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Physical and Chemical Parameters in Assessment of Metabolic Profiles of Patients With B

Martusevich,

Soloveva,

Fedotova

et al. 2024

Innovacionnaâ medicina Kubani

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Objective: To study catalytic properties of lactate dehydrogenase (LDH) and some physical and chemical characteristics of blood in patients with thermal injuries.Materials and methods: We examined blood samples from 24 patients with contact burns involving 10%-40% of the body surface area and studied erythrocyte LDH activity in forward and reverse reactions, kinetic characteristics of this process, and protein concentration. We used teziocrystalloscopy to evaluate crystallogenic properties of blood serum of the examined patients with thermal injuries.Results: Thermal injuries were shown to cause a 2-fold decrease in LDH activity in the reverse reaction. Analysis of crystallogenic properties of blood serum of the patients with contact burns revealed that development of a complex response to a burn injury is accompanied by a moderate decrease in the structure index and crystallizability and a signifi decrease in the clearity of the marginal protein zone. In case of burn disease, we also observed a decrease in the initiative potential of the biological fl In addition, microscope slides of the dried blood serum revealed a tendency to randomization of the sample similar to that found in crystalloscopic facies.Conclusions: The local thermal effect leading to a complex response to a burn injury was found to cause significant transformation of physical and chemical characteristics of blood. Thermal injuries cause a decrease in LDH activity in the reverse reaction leading to a disturbance of the lactate-to-pyruvate ratio in the cell and significant shifts in the blood serum composition and properties, which are manifested in a significant change in blood serum crystallogenic and initiating properties.

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Digital Technology for Processing Dried Drops of Biofluids

Cited by 3 publications

References 0 publications

Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolic state and crystallogenic properties of rat blood plasma: a preclinical experimental study

Physical and Chemical Parameters in Assessment of Metabolic Profiles of Patients With B

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