Digital imaging in pathology: the case for standardization

Yagi, Yukako; Gilbertson, John R.

doi:10.1258/1357633053688705

Cited by 90 publications

(64 citation statements)

References 2 publications

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“…) and for optical density (Yagi and Gilbertson 2005). For morphometry, differences in brightness level or color provide a means to separate stained objects in a specimen image from adjacent non-stained areas (Russ 2010).…”

Section: Study 3: Immuno-peroxidase Density and Surface Area Measuremmentioning

confidence: 99%

An Innovative Method for Obtaining Consistent Images and Quantification of Histochemically Stained Specimens

Linden

Sedgewick²,

Ericson

2015

J Histochem Cytochem.

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SummaryObtaining digital images of color brightfield microscopy is an important aspect of biomedical research and the clinical practice of diagnostic pathology. Although the field of digital pathology has had tremendous advances in whole-slide imaging systems, little effort has been directed toward standardizing color brightfield digital imaging to maintain imageto-image consistency and tonal linearity. Using a single camera and microscope to obtain digital images of three stains, we show that microscope and camera systems inherently produce image-to-image variation. Moreover, we demonstrate that post-processing with a widely used raster graphics editor software program does not completely correct for sessionto-session inconsistency. We introduce a reliable method for creating consistent images with a hardware/software solution (ChromaCal™; Datacolor Inc., NJ) along with its features for creating color standardization, preserving linear tonal levels, providing automated white balancing and setting automated brightness to consistent levels. The resulting image consistency using this method will also streamline mean density and morphometry measurements, as images are easily segmented and single thresholds can be used. We suggest that this is a superior method for color brightfield imaging, which can be used for quantification and can be readily incorporated into workflows. (J Histochem Cytochem 63:233-243, 2015)

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Section: Study 3: Immuno-peroxidase Density and Surface Area Measuremmentioning

confidence: 99%

An Innovative Method for Obtaining Consistent Images and Quantification of Histochemically Stained Specimens

Linden

Sedgewick²,

Ericson

2015

J Histochem Cytochem.

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“…Issues revolve around selection of the area to be photographed, focus, magnification and the number of images that must be taken to provide a representative sample 11 . Recent advances in human telepathology provide for concentrating on areas of an image 36 . With the addition of a video camera to the microscope images can be seen in real-time, but with a loss of resolution.…”

Section: Areas Of Practice In Which Telemedicine Is Usedmentioning

confidence: 99%

Telemedicine in veterinary practice

Mars¹,

Auer²

2006

J. S. Afr. Vet. Assoc.

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Veterinary surgeons have a long tradition of consulting one another about problem cases and many have unwittingly practised telemedicine when discussing cases by telephone or by sending laboratory reports by telefax. Specific veterinary telemedicine applications have been in use since the early 1980s, but little research has been undertaken in this field. The Pubmed and CAB International databases were searched for the following Boolean logic-linked keywords; veterinary AND telemedicine, veterinary AND telecare, animal AND telemedicine, animal AND telecare and veterinary AND e-mail and an additional search was made of the worldwide web, using Google Scholar. This returned 25 papers which were reviewed. Of these only 2 report research. Sixteen papers had no references and 1 author was associated with 13 papers. Several themes emerge in the papers reviewed. These include remarks about the use of telemedicine, the benefits that can and are derived from the use of telemedicine, areas of practice in which telemedicine is being used, ethical and legal issues around the practice of telemedicine, image standards required for telemedicine, the equipment that is required for the practice of telemedicine, advice on ways in which digital images can be obtained and educational aspects of telemedicine. These are discussed. Veterinary practice has lagged behind its human counterpart in producing research on the validity and efficacy of telemedicine. This is an important field which requires further research.

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“…Such a digital transformation in pathology was made possible due to the continuous modernisation of scanning devices, the increasing computers power and the transfer rate coupled with introducing new software solutions and technologies for collecting large amounts of data. One of the important advantages of digital pathology is providing the means to better integrate diagnosis and treatment of cancer by facilitating interaction between multidisciplinary teams of doctors involved in diagnosing and treating these diseases [1][2][3][4][5][6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Future perspectives of digital pathology

Prochorec‐Sobieszek

2016

Nowotwory. Journal of Oncology

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Technological advances have enabled innovative solutions to be achieved in pathology based on digital imaging, now superseding those of conventional microscopy. Digital pathology has been defined as 'virtual microscopy' and depends on computer-generated digital imaging of microscope slides (WSI -whole slide imaging) which are in turn created, reviewed, managed, shared, analysed and interpreted. Such WSI systems and digital consulting platforms are now used for teaching, scientific research, telepathology / teleconsultation and diagnostics. They also permit easy and interactive sharing of WSI that can be integrated into other medical information systems. The software for automated image analysis and computer aided diagnosis can thereby make highly accurate diagnoses and help standardise study findings. Despite the technique's many advantages, its noted drawbacks include high equipment and software costs, image quality issues of standardisation and most importantly, that pathologists are reluctant to use it routinely for making diagnoses. NOWOTWORY J Oncol 2016; 66, 4: 277-285

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Digital imaging in pathology: the case for standardization

Cited by 90 publications

References 2 publications

An Innovative Method for Obtaining Consistent Images and Quantification of Histochemically Stained Specimens

An Innovative Method for Obtaining Consistent Images and Quantification of Histochemically Stained Specimens

Telemedicine in veterinary practice

Future perspectives of digital pathology

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