Digital gene-expression profiling analysis of the fatty liver of Landes geese fed different supplemental oils

Tang, Junwang; Fang, Qiuhong; Shao, Rongyi; Shen, Junda; He, Jun; Niu, Dong; Liu, Lizhi

doi:10.1016/j.gene.2018.05.122

Cited by 8 publications

(5 citation statements)

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“…By comparison, C18:1, particularly C18:1n9, was the most abundant FA in the fatty livers of Landes geese, followed by C16:0 and C18:0. This finding is consistent with recent studies ( Tang et al, 2018 ; Liu et al, 2020 ). C18:1 is the main FA for the formation of TG, which represented more than 45% of the total FA in the liver after 25 days of overfeeding.…”

Section: Discussionsupporting

confidence: 94%

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Metabolic Profiling Analysis of Liver in Landes Geese During the Formation of Fatty Liver via GC-TOF/MS

Lyu

et al. 2022

Front. Physiol.

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Fatty liver production results from the process of overfeeding geese, inducing a dramatic increase in de novo liver lipogenesis. To investigate the alteration of liver metabolites by overfeeding, especially lipid metabolites, and the potential pathways causing these changes, 60 Landes geese at 65 days old were raised in three groups with 20 geese per group, namely, the D0 group (free from gavage), D7 group (overfeeding for 7 days), and D25 group (overfeeding for 25 days). At 90 days old, segments of liver tissue were collected from 10 geese of each group for gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS) analysis. A large number of endogenous molecules in the livers of geese were altered dramatically by overfeeding. In the livers of overfed geese, the level of oleic acid was observed to continuously increase, while the levels of phenylalanine, methyl phosphate, sulfuric acid, and 3-hydroxybenzaldehyde were decreased. The most significantly different metabolites were enriched in amino acid, lipid, and nucleotide metabolism pathways. The present study further supports the idea that Landes geese efficiently produce fatty liver, and potential biomarkers and disturbed metabolic pathways during the process of forming fatty liver were identified. In conclusion, this study might provide some insights into the underlying mechanisms of fatty liver formation.

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Section: Discussionsupporting

confidence: 94%

“…It has been documented that Landes geese have a strong ability to store fat in the liver ( Tang et al, 2018 ). After Landes geese have been overfed for approximately 20 days, their fat level can reach nearly 60% of the goose liver mass ( Hermier et al, 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling Analysis of Liver in Landes Geese During the Formation of Fatty Liver via GC-TOF/MS

Lyu

et al. 2022

Front. Physiol.

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“…The fatty liver in geese, also called foie gras, is looked upon the delicious foods as caviar, black mushroom by the occidental, which has a rich, buttery, and delicate flavor (Tang et al, 2018). Consumers worldwide enjoy it, and there is a huge international market (Zhu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS

et al. 2020

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During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serum of geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach. Sixty Landes geese of the same age were randomly divided into the following three groups with 20 replicates in each group: D0 group (free from gavage); D7 group (overfeeding for 7 days); D25 group (overfeeding for 25 days). At the end of the experiment, 10 geese of similar weight from each group were selected for slaughter and sampling. The results showed that overfeeding significantly increased the body weight and the liver weight of geese. Serum enzymatic activities and serum lipid levels were significantly enhanced following overfeeding. Gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS) was employed to explore the serum metabolic patterns, and to identify potential contributors to the formation of fatty liver and the correlated metabolic pathways. Relative to overfeeding for 7 days, a large number of endogenous molecules in serum of geese overfed for 25 days were altered. Continuous elevated levels of pyruvic acid, alanine, proline and beta-glycerophosphoric acid and reduced lactic acid level were observed in the serum of overfed geese. Pathway exploration found that the most of significantly different metabolites were involved in amino acids, carbohydrate and lipid metabolism. The present study exhibited the efficient capability of Landes geese to produce fatty liver, identified potential biomarkers and disturbed metabolic pathways in liver steatosis. These findings might reveal the underlying mechanisms of fatty liver formation and provide some theoretical basis for the diagnosis and treatment of liver diseases.

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“…This distinct difference in the phenotype indicates that goose fatty liver might become a resource for better understanding lipid deposition in birds and for human fatty liver research. Previous research on the synthesis and delivery of fatty acids focused on goose liver without considering adipose tissues [7, 11, 12]. To explore the dynamic genetic pattern behind the regulation of lipid deposition in goose, we have built a weight-gain model to investigate the mechanism.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis between Normal and high-intake feeding geese provides insight into adipose deposition and susceptibility to fatty liver in migratory birds

Wang

Jin

et al. 2019

BMC Genomics

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Background Dysregulation of adipogenesis causes metabolic diseases, like obesity and fatty liver. Migratory birds such as geese have a high tolerance of massive energy intake and exhibit little pathological development. Domesticated goose breeds, derivatives of the wild greyleg goose ( Anser anser ) or swan goose ( Anser cygnoides ), have high tolerance of energy intake resembling their ancestor species. Thus, goose is potentially a model species to study mechanisms associated with adipogenesis. Results Phenotypically, goose liver exhibited higher fat accumulation than adipose tissues during fattening (liver increased by 3.35 fold than 1.65 fold in adipose), showing a priority of fat accumulation in liver. We found the number of differentially expressed genes in liver (13.97%) was nearly twice the number of that in adipose (6.60%). These differentially expressed genes in liver function in several important lipid metabolism pathways, immune response, regulation of cancer, while in adipose, terms closely related to protein binding, gluconeogenesis were enriched. Typically, genes like MDH2 and SCD, which have key roles in glycolysis and fatty acids metabolism, had higher fold change in liver than in adipose tissues. Three hundred two differentially expressed long noncoding RNAs involved in regulation of metabolism in liver were also identified. For example, lncRNA XLOC_292762 , which was 5.7 kb downstream of FERMT2, a gene involved phosphatidylinositol-3,4,5-trisphosphate binding, was significantly down-regulated after the high-intake feeding period. Further investigation of documented obesity-related orthologous genes in goose suggested that understanding the evolutionary split from mammals in adipogenesis will make goose fatty liver a better resource for future research. Conclusions Our research reveals that goose uses liver as the major tissue to regulate a distinct lipid synthesis and degradation flux and the dynamic expression network analyses showed numerous layers of positive responses to both massive energy intake and possible pathological development. Our results offer insights into goose adipogenesis and provide a new perspective for research in human metabolic dysregulation. Electronic supplementary material The online version of this article (10.1186/s12864-019-5765-3) contains supplementary material, which is available to authorized users.

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Digital gene-expression profiling analysis of the fatty liver of Landes geese fed different supplemental oils

Cited by 8 publications

References 102 publications

Metabolic Profiling Analysis of Liver in Landes Geese During the Formation of Fatty Liver via GC-TOF/MS

Metabolic Profiling Analysis of Liver in Landes Geese During the Formation of Fatty Liver via GC-TOF/MS

A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS

Transcriptomic analysis between Normal and high-intake feeding geese provides insight into adipose deposition and susceptibility to fatty liver in migratory birds

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