Differentiation of thymocytes during chicken ontogeny: occurrence of a specific DNA ligase in relationship to cell size and surface antigens

David, Jean‐Claude; Fedecka-Bruner, Barbara; Mishal, Zohair; Vinson, D.; Rosenfeld, C.

doi:10.1002/eji.1830110713

Cited by 9 publications

(11 citation statements)

References 13 publications

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“…It was also observed that 6 S DNA ligase is expressed only in mature thymocytes characterized by the expression of a T cell differentiation marker [18]. As demonstrated by Le Douarin and co-workers [12,131 the bird thymus is colonized during the embryonic and perinatal life by successive waves of LPC.…”

Section: Discussionmentioning

confidence: 91%

Relationship between thymus ontogeny and DNA ligase expression in the chicken

Jotereau

Maniey

David³

1987

Eur J Immunol

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DNA ligase expression was studied in the embryonic chicken thymus. As previously reported, during thymus ontogeny two separate and successive (8S and 6S) forms of enzyme are found. The switch from the heavy to the light form takes place at around 18 days of incubation in situ. The demonstration that two successive generations of thymic lymphoid precursor cells develop in the embryonic bird thymus and that the replacement of the first by the second thymocyte generation takes place at around 18 days suggested the possibility that each form of enzyme could be restricted to a given thymocyte generation. By either culturing or transplanting embryonic thymuses of different ages it appears that the switch from the heavy to the light form of DNA ligase is not related to a change over of the first by the second generation of embryonic thymocytes but rather to some maturation process of the thymic lymphocytes starting from 17 days of embryonic life. The influence of extrathymic factors on the turning on of the expression of the 6 S DNA ligase in the post-natal environment is also suggested.

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Section: Discussionmentioning

confidence: 91%

Relationship between thymus ontogeny and DNA ligase expression in the chicken

Jotereau

Maniey

David³

1987

Eur J Immunol

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“…For sorting by fluorescence-activated cell sorter (FACS), cells were labeled by indirect immunofluorescence assay with anti-T or anti-B xenoantisera as previously described [15], or with anti-B mAb according to the procedure of Forni [18]. Cell sorting was performed on FACS I1 (Becton Dickinson, Sunnyvale, CA) according to cell size or fluorescence as already described in a previous report [15].…”

Section: Cell Labeling and Sortingmentioning

confidence: 99%

“…Cell sorting was performed on FACS I1 (Becton Dickinson, Sunnyvale, CA) according to cell size or fluorescence as already described in a previous report [15]. The sorted cells were sedimented for 5 min at 1200 rpm and the supernatant was discarded.…”

Section: Cell Labeling and Sortingmentioning

confidence: 99%

“…Two hundred and fifty pl of this extract were layered on the top of a sucrose gradient, 5-20%, in 0.5 M NaCl, 50 mM Tris-HC1, pH 7.4, 1 mM EDTA, 2 mM dithiothreitol and centrifuged for 15 h at 47000 rpm in a SW50.1 rotor at 4°C. DNA ligase was tested as described elsewhere [15]. One unit of DNA ligase activity is defined as the activity which renders 1 nmol of [32P]oligo(dT) resistant to alkaline phosphatase in 30 min.…”

Section: Dna Ligase Assaymentioning

confidence: 99%

“…In a previous report [15], we proposed a marker of a late stage of thymocyte differenciation in chicken, the activity of a light ( 6 s ) DNA ligase. Indeed, this form of enzyme appears in thymocytes before hatching, whereas the heavy form (8 S) of DNA ligase, present during earlier stages of development, decreases in activity and eventually disappears.…”

Section: Introductionmentioning

confidence: 99%

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DNA ligases as markers of lymphoid cell maturation and heterogeneity in the chicken

1985

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The activities of 8 S and 6 S DNA ligases have been studied in the chicken lymphoid cells of blood, spleen and bursa of Fabricius at different stages of development, from late embryonic life to about 3 months after hatching. These cells have been sorted with the fluorescence-activated cell sorter FACS II on the basis of size and T or B antigenicity (immunofluorescence). The light 6 S DNA ligase has been previously demonstrated to be associated to a late stage of differentiation of thymocytes. In the bursa, a unique form of 8 S DNA ligase is found during the whole period of observation. This form of enzyme remains in the B cells of the spleen until 3 weeks after hatching, but is never present in the blood B cells. As far as T cells are concerned, the light DNA ligase is present in the blood from 18-day embryonic life on. In the spleen T cells, on the contrary, this enzyme appears only 3 weeks after hatching. Before this stage, splenic T cells are devoid of any form of DNA ligase activity. These findings show biochemical differences in T and B lymphocytes colonizing the periphery, blood and spleen, and suggest, at least for the T cells at early stages, a heterogeneity in the degree of differentiation.

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Ontogeny of primary lymphoid organs and lymphoid stem cells

1984

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Cells of the immune system go through a series of important developmental steps that begin early in embryonic life and include, first, the various waves of hemopoietic-cell production in the embryo and, second, the homing of these cells to the hemopoietic organs, which are the sites of hemopoiesis and lymphopoiesis in embryonic and adult life. The avian embryo is a n important model for investigating these early steps; and this paper presents a comprehensive review of the work done on the early ontogeny of the avian immune system.

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Differentiation of thymocytes during chicken ontogeny: occurrence of a specific DNA ligase in relationship to cell size and surface antigens

Cited by 9 publications

References 13 publications

Relationship between thymus ontogeny and DNA ligase expression in the chicken

Relationship between thymus ontogeny and DNA ligase expression in the chicken

DNA ligases as markers of lymphoid cell maturation and heterogeneity in the chicken

Ontogeny of primary lymphoid organs and lymphoid stem cells

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