Differentiation of Rat Mesenchymal Stem Cells toward Osteogenic Lineage on Extracellular Matrix Protein Gradients

Ghaemi, Soraya Rasi; Delalat, Bahman; Cavallaro, Alex; Mierczyńska-Vasilev, Agnieszka; Vasilev, Krasimir; Voelcker, Nicolas H.

doi:10.1002/adhm.201900595

Cited by 11 publications

(12 citation statements)

References 37 publications

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“…Combing different kinds of biomaterials with cells to repair and finally restore tissue functions is the current tissue regeneration approaches (Wang et al, 2019; Yang et al, 2019). The aim of bone tissue engineering is to guide stem cells to differentiate towards osteogenic lineage (Li et al, 2012; Menaa, Abdelghani, & Menaa, 2015; Rasi Ghaemi et al, 2019). To promote bone regeneration, the effective promotion of the osteogenic differentiation of rBMSCs on 3D SF scaffolds incorporated with graphene is being studied.…”

Section: Discussionmentioning

confidence: 99%

Three‐dimensional silk fibroin scaffolds incorporated with graphene for bone regeneration

Ding

Huang

et al. 2020

J Biomedical Materials Res

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Porous three‐dimensional (3D) silk fibroin (SF) scaffolds were widely applied for bone regeneration and showed excellent biocompatibility and biodegradability. Recently graphene was developed for bone scaffolds due to its osteogenic properties. Thus, we combine the SF and graphene to improve the osteogenic properties of SF scaffolds. In our study, we explored the incorporation of SF scaffolds with graphene to develop osteogenic scaffolds capable of accelerating bone formation. The 3D SF scaffolds were fabricated with different contents of graphene (0, 0.5, and 2%). Fluorescence images showed that the graphene nanosheets were homogeneously dispersed in the SF scaffolds. The addition of graphene affected the microarchitecture of the scaffolds. The G/SF scaffolds were cocultured with rat bone marrow‐derived mesenchymal stem cells (rBMSCs) for 21 days. The cell morphology and cell proliferation study suggested that 0 and 0.5% G/SF scaffolds displayed good cell proliferation. In addition, immunofluorescent staining (e.g., osteonectin, osteopontin, and osteocalcin) and ALP activities indicated that the osteogenic properties was more actively exhibited on 0.5% G/SF scaffolds compared with the other groups. Our results indicated that SF scaffolds incorporated with graphene could be an appropriate scaffold for bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Three‐dimensional silk fibroin scaffolds incorporated with graphene for bone regeneration

Ding

Huang

et al. 2020

J Biomedical Materials Res

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“…Surfaces with different kinds of (bio)chemistry have been prepared, for example, density of poly(ethylene glycol) brushes, 266 Fn, 289 , 239 laminin, 235 gelatin, 290 collagen type I (COL1), and osteopontin (OPN), 291 osteogenic growth peptide (OGP), 246 RGD, 244 −CH 3 , 218 −COOH, 219 plasma polymerized hexane, 225 Acrylic acid/diethylene glycol dimethyl ether, 231 PLLA/PDLLA, 254 poly(caprolactone) and poly( d , l -lactide), 292 poly(2-hydroxyethyl methacrylate). 240 Mohan et al 218 fabricated chemical gradients on the surface of PDMS, and water contact angle (WCA) increased from ∼10° to ∼100°.…”

Section: Gradient-based Hts Approaches For Biomaterials Discovery and Materiobiologymentioning

confidence: 99%

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

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“…Gradient of collagen concentration can promote bone differentiation of MSCs. [ 95 ] Increasing collagen concentration can increase Runx2 of MSCs, demonstrating that higher integrin adhesion can promote osteogenesis. Cell differentiation has also been studied through changes in ligand density in the hydrogel.…”

Section: Mechanobiological Strategies To Control Stem Cell Differentimentioning

confidence: 99%

Mechanical Properties of Materials for Stem Cell Differentiation

et al. 2020

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Traditionally, understanding differentiation pathway has been achieved by studying biochemical signal pathways controlled by various growth factors and cytokines. However, since various physical factors including tissue stiffness and topology can also determine the differentiation pathway of stem cells, mechanobiological pathway for controlling differentiation has been emphasized. Moreover, newly identified mechanobiological pathways have encouraged efforts to interpret stem cell differentiation in terms of cell-material interaction and provided clues to accurately design microenvironment of stem cells to control the direction of differentiation. Cells continuously recognize topographical and mechanical properties of the surrounding microenvironment and modulate their functional phenotypes through appropriate physiological responses to maintain homeostasis. Cell-cell and cell-extracellular matrix (ECM) interactions determine physical connections between the outside and the inside of individual cells to regulate various cellular functions, including adhesion, migration, proliferation, and cell differentiation. [3] Integrin, a transmembrane protein, is actively involved in outside-in and inside-out signaling mediated by polymerization and contraction of the cytoskeleton known to control cellular mechanotransduction pathways. [4] Therefore, a changed physical microenvironment can be detected by integrin-ECM interaction which has been traditionally considered as a primary target for controlling cell behavior through the material properties. Recent studies have shown that nuclear mechanosensation is a key process in response to physical stimuli. Nuclear membrane is tightly connected to integrin-based focal adhesion through cytoskeletal fibers that can transmit external force or cytoskeletal tension to the nuclear membrane, causing structural deformation of the nucleus. [5] Applied force not only changes nuclear shape, but also determines the conformation of many proteins located in nuclear membrane associated with various biochemical signals. [6] Since transcriptional regulatory mechanisms, such as histone modification and transcription factor activity, are controlled by force-mediated nuclear deformation, signal pathways for nuclear mechanosensation have been focused to interpret cellular adaptation mechanism including stem cell differentiation. [7] Various methods have been used to determine cell functions by changing cell adhesion through the corresponding changes in external substrates using micropatterned cell confinement, micro-/nanosized topographic substrates, and substrate stiffness. Micropatterning of ECM proteins is a well-established method to Recent findings about cell fate change induced by physical stimuli have expedited the discovery of underlying regulatory mechanisms that determine stem cell differentiation. Progress with regards to micro-/nanofabrication technology have led to the development of advanced materials that can mimic biophysical features of in vivo related circumstances of the human body...

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Differentiation of Rat Mesenchymal Stem Cells toward Osteogenic Lineage on Extracellular Matrix Protein Gradients

Cited by 11 publications

References 37 publications

Three‐dimensional silk fibroin scaffolds incorporated with graphene for bone regeneration

Three‐dimensional silk fibroin scaffolds incorporated with graphene for bone regeneration

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

Mechanical Properties of Materials for Stem Cell Differentiation

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