Differentiation of mycobacterium tuberculosis and mycobacterium bovis by p-nitrobenzoic acid susceptibility

Tsukamura, M; Tsukamura, S

doi:10.1016/s0041-3879(64)80091-x

Cited by 71 publications

(25 citation statements)

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“…Colony morphology and the ability to grow at various temperatures (24,31,37, and 45°C) were determined after 1 week of incubation on Loewenstein-Jensen medium slants. The following properties were determined as described previously: the niacin test was performed by using test strips (Difco Laboratories, Detroit, Mich.) (18,37); nitrate reductase activity was determined by the method of Kubica and David (19); catalase activity at 68°C was determined by the method of Kubica and Pool (20); arylsulfatase activity after 3 days was determined by the method of Kubica and Ridgon (21); p-glucosidase and P-galactosidase activities were determined by the method of Tsukamura (31); Tween 80 hydrolysis was determined by the method of Wayne et al (36); growth on MacConkey agar was determined by the method of Pattyn and Portaels (25); resistance to NaCl and p-nitrobenzoic acid was determined by the methods of Wayne et al (36) and Tsukamura and Tsukamura (34); acetamidase, allantoinase, benzamidase, nicotinamidase, pyrazinamidase, succinamidase, and urease activities were determined by the method of Bonicke (2); and tests to determine resistance to streptomycin Tests to determine decomposition of adenine, guanine, hypoxanthine, xanthine, tyrosine, elastine, keratine, and testosterone were performed by the method of Gordon and Smith (15); tests to determine esculin decomposition were performed by the method of Gordon (13); and tests to determine casein and gelatin hydrolysis were performed by the method of Gordon and Mihm (14). Urea decomposition was tested by using urea agar base (code CM 53; Oxoid) after 2.2% urea was added.…”

Section: Methodsmentioning

confidence: 99%

Tsukamurella inchonensis sp. nov.

Yassin

Rainey²,

Brzezinka³

et al. 1995

International Journal of Systematic Bacteriology

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Chemotaxonomic and genomic 16s ribosomal DNA sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus TsukamureZlu. This new species can be identified by its 16s ribosomal DNA similarity values, as well as its physiological characteristics. The name TsukamureZh inchonensis sp. nov. is proposed for these isolates, which are represented by strain IMMIB D-771T (= DSM 44067T) (T = type strain). This strain exhibits only 45% DNA relatedness to Tsukamurella pauro-

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Section: Methodsmentioning

confidence: 99%

Tsukamurella inchonensis sp. nov.

Yassin

Rainey²,

Brzezinka³

et al. 1995

International Journal of Systematic Bacteriology

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“…If growth occurred, it was transferred to a fresh Ogawa egg slant medium (control) and another Ogawa egg slant medium containing 0.5 mg/ml p-nitrobenzoic acid [25]. To prepare the latter medium, p-nitrobenzoic acid was first dissolved in propylene glycol at a concentration of 25 mg/ml, and one volume of this solution was added to 50 volumes of the Ogawa egg medium before sterilization.…”

Section: Isolationmentioning

confidence: 99%

A Comparative Study of Mycobacteria from Patients' Room Dusts and from Sputa of Tuberculous Patients

Tsukamura

Matsumoto

Murata

et al. 1974

Japanese Journal of Microbiology

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The source of mycobacteria other than Mycobacterium tuberculosis occurring in sputa of tuberculous patients as casual isolates was investigated by comparing the occurrence rate of mycobacterial species between patient's room dust and patient's sputum. Almost all species of mycobacteria recovered from sputa could be found in dusts obtained from the rooms. However, the percentage of occurrence of the mycobacterial species in dusts differed from that in sputa. In dusts, Mycobacterium fortuitum (39.6%), Mycobacterium nonchromogenicum (23.5%) and Mycobacterium gordonae (16.7%) occurred in high frequencies, whereas Mycobacterium intracellulare (69.6%), M. gordonae (5.9%), Mycobacterium scrofulaceum (5.2%), and Gordona bronchialis (10.4%) were the main species found in sputa. The patterns of occurrence of mycobacterial species as illustrated above may suggest that pathogenic mycobacteria survive in the respiratory tract while nonpathogenic ones are destroyed there, thus the human body acts as a selective medium for the pathogenic mycobacterial species. Serotype studies on strains of M. intracellulare as casual isolates indicated that those from sputa of tuberculous patients were different from those derived from patients with lung diseases due to this species of mycobacteria.These results led us to the conclusion that mycobacteria in patients' room dusts were the source of mycobacteria occurring in sputa as casual isolates, particularly the pathogenic mycobacteria in dusts are more likely to survive in the human respiratory tract, occasionally multiplying and causing disease under favorable circumstances.

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“…NOTES ria; (2) no growth on Sauton agar containing 0.1 % NaNO2 or on that containing 0.2% picric acid (pH 7.0) [12] ; (3) no reduction of nitrate to nitrite [12] ; (4) Tween not hydrolyzed at 14 days [15] ; (5) resistance to 5 [12] ; (6) strong alpha-and beta-esterase activity [4] ; (7) acetate and pyruvate utilization, but no succinate, malate and fumarate utilization as carbon source in the presence of ammoniacal nitrogen [12] ; (8) resistance to 0.5 mg/ml NH2OH.HCl in Ogawa egg medium [12] ; (9) resistance to 0.5 mg/ml p-nitrobenzoic acid in Ogawa egg medium [14] ; (10) no niacin production [5] ; (11) weak catalase activity (foam height lower than 45 mm) [6]. The above identification does not differentiate between M. avium and M. intracellulare [12].…”

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confidence: 99%