Differentiation of Genuines and Counterfeits ofCordycepsSpecies using Random Amplified Polymorphic DNA

Abstract

For identification of genuine and counterfeit samples of Cordyceps sinensis, an RAPD (random amplified polymorphic DNA) analysis was performed. In the study, twelve samples were collected, of which six were genuine and six were counterfeit samples. Genuine samples of Cordyceps sinensis contain two parasitic larvae produced from each of the three provinces, Sichuan (SC), Chinghai (CH), and Tibet (TB). The counterfeits contain two products of each of the three species, Cordyceps hawkesii, Stachys geobombycis, and Stachys sieboldii. In the RAPD analysis, twenty arbitrary decamer primers were screened to obtain primers appropriate for differentiating the genuine and counterfeit samples. The result of twelve RAPD markers generated from four primers, OPT-08, OPT-12, OPT-13, and OPT-17, were selected. The primers can easily distinguish between genuine and counterfeit samples of Cordyceps sinensis.

RAPD-based genetic relationships in different Bougainvillea cultivars

“…In recent years, several molecular approaches have become available, among them RAPD is most commonly used for the identification of cultivar/variety due to its simplicity, rapidity and requirement of only a small quantity of DNA to generate numerous polymorphisms (Wight et al 1993, Cheng et al 1997. The RAPD assay has been successfully used for studying genetic diversity of many crop species such as kenaf (Zhou et al 2002), rose (Debener et al 1996, Martin 2001), chrysanthemum (Sheng et al 2000 Amaranthus (Faseela and Joseph 2007) etc.…”

RAPD Based Assessment of Genetic Diversity of Adhatoda vasica Leaves from Different Sub- Continents of India

“…Due to easiness, rapidity and simplicity the RAPD technique has been used widely for differentiating a large number of medicinal species from their close relatives or adulterants including Panax species, 25 Coptis species, 26 Astragalus species, Lycium barbarum L., 27 Panax ginseng 28 and Echinacea species. 29 These characters were especially advantageous for the identification of any herbal drug because of little DNA existing in the dried material.…”

Identifi cation of Species-Diagnostic Inter Simple Sequence Repeat Markers for Ten Phyllanthus Species

“…Molecular marker analysis has been widely used for differentiation of a large number of medicinal plant species from their close relatives or adulterants (Shaw and But, 1995;Cheng et al, 1997Cheng et al, , 2000Kochieva, 1999;Sasikumar et al, 2004). Scheef et al (2003) identifi ed two RAPDbased species-specifi c markers for two Agrostis species (A. capillaries -colonial type and A. palustris -creeping type) and subsequently developed (SCAR) markers, which were tested in 17 cultivars belonging to four species.…”