Identifi cation of Species-Diagnostic Inter Simple Sequence Repeat Markers for Ten Phyllanthus Species

Abstract

Phyllanthus has been widely used in traditional medicine as an antipyretic, a diuretic, and to treat liver diseases and viral infections. Correct genotype identifi cation of medicinal plant material remains important for the botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated the need for newer methods in quality control of botanicals. In the present study, attempts were made to identify species-diagnostic markers for ten Phyllanthus species using the inter simple sequence repeat-polymerase chain reaction (ISSR-PCR) fi ngerprinting method. PCR amplifi cation using seven ISSR primers resulted in signifi cant polymorphism among the populations from different species. P. angustifolius and P. urinaria showed monomorphic frequency of maximum (63.88%) and minimum (20.64%), respectively. Seventeen species-diagnostic markers were identifi ed for seven species (P. acidus, P. emblica, P. fraternus, P. urinaria, P. rotundifolius, P. amarus, and P. angustifolius) while no marker was detected for P. reticulatus, P. nivosus, and P. virgulatus. A maximum of six species-diagnostic markers were identifi ed for P. acidus and a minimum of only one of 755 bp was available for P. amarus. Among the seventeen markers, nine were present in all individuals of particular species. The speciesspecifi c differences in fragment numbers and sizes could be used as diagnostic markers to distinguish the Phyllanthus species quickly.

A Robótica Como Estratégia No Desenvolvimento Das Habilidades Do Indivíduo Com Transtorno Do Espectro Autista

“…A P. niruri é uma pequena erva rasteira que tem de 30 a 40 cm de altura e é nativa de regiões tropicais e subtropicais. É conhecido pela sua capacidade de crescer entre as pedras, rachaduras, paredes e muros e em diferentes estações do ano (LEÃO et al, 2017;SENAPATIA;APARAJITA;ROUT;. A espécie possui uma ampla variedade de constituintes farmacológicos e fitoquímicos identificados, como os triterpenóides e alcaloides.…”