RAPD Based Assessment of Genetic Diversity of Adhatoda vasica Leaves from Different Sub- Continents of India

Garg, Madhukar; Tamboli, Ennus Tajuddin; Singh, Mhaveer; Chester, Karishma; Abdin, Malik Zainul; Chandna, Ruby; Ahmad, Sayeed

doi:10.5530/ijper.49.3.9

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…When the ITS amplicons of the four selected Justicia species were subjected to restricted digestion with EcoRI or SfoI, it yielded the expected restricted products (Table 6). Based on earlier reports, it is evident that the variation in J. adhatoda is very high along with a phenotypic plasticity 35,36 . However, J. beddomei seems to be more uniform than J. adhatoda.…”

Section: Resolving Taxonomic Dispute For Justicia Beddomeimentioning

confidence: 82%

Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers

et al. 2018

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The extent of genetic diversity within a species is an important determinant of successful adaptation to adverse environmental conditions. Assessment of extent of genetic diversity/variability is also important to monitor genetic erosion within a species. In threatened plant species, genetic diversity assessment helps in selection of genetically diverse populations to enrich the genetically impoverished populations, thus minimizing the probability of genetic drift. Confirming taxonomic identity of threatened species, particularly those belonging to species complexes with dispute identity, is another essential task in the conservation of threatened species, which is best resolved through molecular approaches. The present study estimated the genetic variability within and among the populations of four threatened species, viz. Justicia beddomei (C.B. Clarke) Bennet (Acanthaceae), Embelia ribes Burm. f. (Myrsinaceae), Madhuca insignis (Radlk.) H.J. Lam (Sapotaceae) and Cycas beddomei Dyer (Cycadaceae) using Inter Simple Sequence Repeat (ISSR) and Simple Sequence Repeat (SSR) markers for selecting the genetically diverse populations. The phylogeny was analysed through ITS (nrDNA) and matK (cpDNA) sequences to confirm the species identity.The phylogenetic analyses confirmed four distinct species of Justicia, which also revealed that J. beddomei and J. adhatoda were sister groups with a common ancestor showing rapid parallel speciation with J. gendarussa in one clade and J. betonica in another. Madhuca insignis with extremely small population in the Western Ghats (Karnataka to Kerala) might have undergone either extensive hybridization or incipient speciation. In case of Embelia species, a greater evolutionary closeness between E. subcoraceae and E. floribunda was revealed, while E. ribes had a distinct clad. Both ISSR and SSR markers distinguished various genotypes of Cycas beddomei.

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Section: Resolving Taxonomic Dispute For Justicia Beddomeimentioning

confidence: 82%

Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers

et al. 2018

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“…Products from PCR amplification using five primers give a high polymorphism value, indicated by a percentage value of 90%. According to Garg et al (2015) the tested plants have a fairly high diversity so as to provide a high value of polymorphism.…”

Section: Molecular Characterization Of Ramiementioning

confidence: 99%

Morphology and Molecular Characterization of Four Types of Ramies (Boehmeria nivea (L.) Gaud) Collection Experimental Farm Faculty of Agriculture Andalas University

Yulfa¹,

Mayerni²,

Yusniwati³

2019

IJEAB

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The purpose of this study was to collect and determine the morphological and molecular character of the ramies in West Sumatra. This study was conducted in May-October 2018 Experimental Farm, Faculty of Agriculture, University of Andalas Padang. This a ctivity also used the clone Padang 3 and Ramindo 1 as a comparison. The results of this study indicated that the ramie plant could grow to a height of 1058 meters above sea level to the point where the coordinates 0 ° 15'30 "South Latitude and 100 ° 15'45" east longitude. Ramie highest Situjuah accession 150.03 cm and the lowest at 91.7 cm Matur accession. The longest and widest leaf observations contained in the accession Situjuah while the shortest and smallest obtained at Matur accession. On accession Situjuah, Matur, and clones Ramindo 1 had male and female flowers but clone Padang 3 only had female flowers. Harvesting fastest in clones Ramindo 1 was 123 days and the longest in clones Padang 3 was 133 days. The numbers of banding pattern resulting from PCR amplification were 74 tapes (68 a polymorphic bands while the rest was the number of monomorphic bands).

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“…Figure 7. Distribution of the number of studies of binding with human serum albumin by the value of the obtained molarity-based association constant for (a) indomethacine[37][38][39][40][41][42][43][44][45][46][47], (b) phenylbutazone[27,28,37,[48][49][50][51][52][53][54][55][56], (c) quercetin[38,[57][58][59][60][61][62][63][64][65][66][67][68][69][70], (d) warfarin[53,56,[71][72][73][74][75][76][77][78][79][80]…”

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confidence: 99%

Binding Constants of Clinical Drugs and Other Organic Ligands with Human and Mammalian Serum Albumins

2021

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A dataset containing the experimental values of the equilibrium binding constants of clinical drugs, and some other organic ligands with human and mammalian (predominantly bovine) serum albumins, is assembled. The affinity of drugs to albumin governs their pharmacokinetic properties, related to permeability through physiological barriers and distribution within the organism. The dataset contains 1755 records gathered from 346 original literature sources describing the albumin affinity of 324 different substances. The data were extracted from both articles and existing protein-binding databases applied strict data selection rules in order to exclude the values influenced by the third-party compounds. The dataset provides the details on the experimental conditions of the measurements, such as temperature; protein and ligand concentrations; buffer pH, composition and concentration; and the method and model used for the binding constant calculations. Analysis of the data reveals discrepancies between the values from different studies, as well as the significant influence of the measurement method. Averaging the values from multiple independent measurements from the dataset may help to determine the reliable values of the binding constants. The dataset can be used as the reference dataset for the development of predictive models to calculate binding constants, and as the choice for the experimental setup in the future albumin-binding studies.

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RAPD Based Assessment of Genetic Diversity of Adhatoda vasica Leaves from Different Sub- Continents of India

Cited by 3 publications

References 17 publications

Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers

Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers

Morphology and Molecular Characterization of Four Types of Ramies (Boehmeria nivea (L.) Gaud) Collection Experimental Farm Faculty of Agriculture Andalas University

Binding Constants of Clinical Drugs and Other Organic Ligands with Human and Mammalian Serum Albumins

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