Differentiation of four taxa of the Anopheles balabacensis complex using H-banding patterns in the sex chromosomes

Abstract

W~eowo, S., V. BAIMAI, and R. G. . Differentiation of four taxa of the Anopheles balabacensis complex using H-banding patterns in the sex chromosomes. Can. J . Genet. Cytol. 26: 425-429. Analyses of metaphase chromosomes of four taxa of the Anopheles balabncensis complex ( A . dirus A, B, and C, and A. takasagoensis) using the Hoechst 33258 staining technique have revealed remarkable differences in the fluorescence banding patterns of the sex chromosomes. These result from changes in the amount and distribution of constitutive heterochromatin. This evidence supports the results from cross-mating experiments and from morphological studies which indicate that three of these taxa, A. takasagoensis, dirus A, and dirus B, are sibling species. Differences in H-staining patterns of the sex chromosomes of a dirus colony from Kanchanaburi suggest that it too is a genetically distinct taxon, provisionally designated as dirus C, within the A. balabacensis complex.

Six new species of the Anopheles leucosphyrus group, reinterpretation of An. elegans and vector implications

“…Other studies of the Dirus and Leucosphyrus Complexes showed that they were groups of apparently isomorphic but reproductively distinct species. Discovery of most members of the Dirus Complex was achieved primarily with crossing studies, cytogenetics (chromosome banding patterns), allozyme data, DNA probes and DNA hybridization (Hii, 1984b; Wibowo et al ., 1984; Baimai et al ., 1987, 1988a; Panyim et al ., 1988; Green et al ., 1992; Audtho et al ., 1995). Cytogenetic studies on members of the Leucosphyrus Group in Thailand and peninsular Malaysia showed differences in the amount and distribution of heterochromatin in the sex chromosomes, suggesting that An.…”

“…comprised at least four genetically distinct genetic species: these were designated An. dirus A, B, C and D (Baimai et al ., 1981, 1984; Hii, 1982; Wibowo et al ., 1984). Kanda et al .…”

“…Anopheles dirus B of Baimai et al ., 1984: 536 (metaphase chromosomes); Wibowo et al ., 1984: 425 (H‐banding pattern of sex chromosomes); Peyton, 1990: 197 (taxonomy); Klein et al ., 1991: 481 (susceptibility to P. cynomolgi B strain); Prapanthadara et al ., 2000: 395 (glutathione S‐transferase).…”

“…cracens ). Additional studies of cytogenetics and hybridization (Baimai et al ., 1984, 1988a,d; Hii, 1984b, 1985; Wibowo et al ., 1984; Baimai & Traipakvasin, 1987; Baimai, 1988a,b; Poopittayasataporn & Baimai, 1995), plus morphological and morphometric characters of the immature stages, were taken to confirm the specific status of An. cracens within the Dirus Complex (Hii, 1986; Damrongphol & Baimai, 1989).…”

“…Anopheles dirus species C of Baimai et al ., 1984: 536 (cytogenetics); Wibowo et al ., 1984: 425 (H‐banding pattern of sex chromosomes); Baimai et al ., 1987: 157 (crossing, chromosomes); May, 1987: 886 (neuroblast chromosomes); Baimai, 1988a: 13 (heterochromatin, evolution, distribution); Baimai, 1988b: 667 (population cytogenetics); Baimai et al ., 1988d: 372 (polytene chromosomes); Yasothornsrikul et al ., 1988: 703 (RFLP); Damrongphol & Baimai, 1989: 563 (egg morphology); Peyton, 1990: 197 (taxonomy); Sawadipanich et al ., 1990: 477 (cytogenetics, crossing, evolution); Green et al ., 1992: 29 (enzyme electromorphs); Audtho et al ., 1995: 107 (DNA hybridization); Poopittayasataporn & Baimai, 1995: 426 (polytene chromosomes, phylogeny); Walton et al ., 1999: 24 (ASA PCR species identification); Walton et al ., 2000: 962 (mtDNA genetic structure); Walton et al ., 2001: 569 (population genetics, microsatellites); Huong et al ., 2001: 615 (PCR species identification); Manguin et al ., 2002: 46 (SCAR markers, multiplex PCR).…”