Differential uptake of neutral red by macrophages from three species of estuarine fish

Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties

“…The absorbance of the α-glucan solution was read at 510 nm against a reagent blank. The content of total polysaccharides was determined by phenol-sulphuric acid assay according to Dubois et al [7]. 0.1 g of DM, DB, IPSs and EPSs was diluted into 1 mL distilled water in test tube.…”

Antitumor and immunomodulating activity of a polysaccharide from Sophora flavescens Ait.

“…Phagocytosis of peritoneal macrophages was measured according to the reported method with modification [17,18]. Briefly, peritoneal macrophages were harvested by peritoneal lavage from BALB/c mice 3 days after i.p.…”

Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis

“…After 3 days, the resident macrophages were harvested by peritoneal lavage and centrifugation (4°C, 1000 rpm, 5 min). Then peritoneal macrophages were cultured in complete RPMI 1640 medium in 96-well plate for 2 h at 37°C in a [18]. All determinations were conducted in triplicate.…”