Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties

Ubaidillah, Nik Hafizah Nik; Abdullah, Noorlidah; Sabaratnam, Vikineswary

doi:10.1016/j.ejbt.2015.03.006

Cited by 21 publications

(8 citation statements)

References 16 publications

(17 reference statements)

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“…The supernatant was discarded, and the pellet was placed in a food dehydrator at the lowest temperature until a constant weight was achieved. The IPS yield was estimated by multiplying the dilution factor by the IPS weight [14].…”

Section: Methodsmentioning

confidence: 99%

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Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology

et al. 2019

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Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect ( p < 0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value ( p < 0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum .

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Section: Methodsmentioning

confidence: 99%

“…Fungal polysaccharide exists in two forms, exopolysaccharide (EPS) and intracellular polysaccharide (IPS). EPS is secreted outside the mycelium whereas IPS is produced inside the mycelium [10],[14]. Generally, total polysaccharide content produced by the mushroom thus comprises both EPS and IPS.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology

et al. 2019

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“…42 In other mushroom species, intracellular and extracellular polysaccharides extracted from Ganoderma neojaponicum (Imazeki) enhanced RAW264 proliferation and increased phagocytosis, with no toxicity observed in SpragueeDawley rats. 43 Kozarski et al 44 reported that extracts from spores of Ganoderma lucidum increased IFN-g in stimulated human peripheral blood mononuclear cells after 72 h.…”

Section: Immunopotentiators Extracted From Mushroomsmentioning

confidence: 99%

Natural Vaccine Adjuvants and Immunopotentiators Derived From Plants, Fungi, Marine Organisms, and Insects

Woods

Niwasabutra

Acevedo

et al. 2017

Immunopotentiators in Modern Vaccines

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“…Auch Fütterungsversuche an Ratten, die einen Extrakt aus den Fruchtkörpern erhielten und über 14 Tage beobachtet wurden, zeigten keine Vergiftungssymptome. Die LD50 (Ratte, oral) wurde mit > 2000 mg/kg bestimmt [13]. Intoxikationen beim Menschen werden möglicherweise nur selten erkannt, wenn sie subklinisch verlaufen und von einem “Heilpilz” nicht erwartet werden.…”

Section: Bedrohliche Neurologische Symptome Nach Morchelverzehrunclassified

Wenig bekannte Pilzvergiftungen

Berndt

2016

Biologie in unserer Zeit

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Die Arbeit gibt einen Überblick über neuere Pilzvergiftungen, ihre Symptome und Toxine, soweit bekannt. Vergiftungen sind meist Folge von Verwechslungen mit Speise‐ oder “Heilpilzen”. Giftige Arten, wie der Große Stachelschirmling, Ohrförmige Weißseitling, Parfümierte Trichterling und der Ölbaumtrichterling kommen in Mitteleuropa vor. Wenig bekannt ist, dass reichlicher Morchelgenuss zu neurologischen Symptomen führen kann. Aus Asien sind Arten bekannt geworden, die schwerste Intoxikationen hervorrufen. Das “Yunnan Sudden Unexplained Death”‐Syndrom konnte mit dem Verzehr des “little white mushroom” (Trogia venenata) erklärt werden. Die oft tödlich verlaufenden Rhabdomyolysen nach Verzehr von Russula subnigricans beruhen auf Cycloprop‐2ene‐Karbonsäure. Besonders folgenschwer sind Verwechslungen vom als “Pilz der Unsterblichkeit” geschätzten “Reishi” mit Ganoderma neo‐japonicum oder Podostroma cornu‐damae, dem weltweit giftigsten Pilz.

show abstract

Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties

Cited by 21 publications

References 16 publications

Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology

Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology

Natural Vaccine Adjuvants and Immunopotentiators Derived From Plants, Fungi, Marine Organisms, and Insects

Wenig bekannte Pilzvergiftungen

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