Differential responses of gut microbiota of male and female fathead minnow (Pimephales promelas) to a short-term environmentally-relevant, aqueous exposure to benzo[a]pyrene

DeBofsky, Abigail; Xie, Yuwei; Grimard, Chelsea; Alcaraz, Alper James; Brinkmann, Markus; Hecker, Markus; Giesy, John P.

doi:10.1016/j.chemosphere.2020.126461

Cited by 38 publications

(16 citation statements)

References 66 publications

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“…Assessment of the 20 most common families, genera, and species of bacteria in control fathead minnows showed that 70% were Proteobacteria, 10% Bacteriodetes, and 10% Fusobacteria (Narrowe et al 2015). In another study, fathead minnow controls were dominated by the phyla Proteobacteria and Fusobacteria (Bridges et al 2018; DeBofsky et al 2020). The most abundant families vary between studies of fathead minnows: Aeromonadaceae (phylum Proteobacteria) and Shewanallaceae (phylum Proteobacteria) were dominant in Narrowe et al (2015), whereas Burkholderiaceae (phylum Proteobacteria) and Fusobacteriaceae (phylum Fusobacteria) were dominant in DeBofsky et al (2020).…”

Section: Discussionmentioning

confidence: 99%

Chronic Embryo‐Larval Exposure of Fathead Minnows to the Pharmaceutical Drug Metformin: Survival, Growth, and Microbiome Responses

Parrott

Restivo

Kidd

et al. 2021

Enviro Toxic and Chemistry

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Metformin is a glucose-lowering drug commonly found in municipal wastewater effluents (MWWEs). The present study investigated the chronic effects of metformin in early-life stages of the fathead minnow (Pimephales promelas). Endpoints assessed were growth, survival, and deformities. The larval gut microbiome was also examined using 16 S ribosomal RNA gene amplicon sequencing to determine microbial community composition and alpha and beta diversity. Eggs and larvae were exposed to metformin measured concentrations (mean [standard deviation]) of 0.020 (0.017) μg/L (for controls) and 3.44 (0.23), 33.6 (1.6), and 269 (11) μg/L in a daily static-renewal setup, with 20 embryos per beaker. The low and middle metformin exposure concentrations represent river and MWWE concentrations of metformin. To detect small changes in growth, we used 18 replicate beakers for controls and 9 replicates for each metformin treatment. Over the 21-d exposure (5 d as embryos and 16 d posthatch [dph]), metformin did not affect survival or growth of larval fish. Hatch success, time to hatch, deformities in hatched fry, and survival were similar across all treatments. Growth (wet wt, length, and condition factor) assessed at 9 and 16 dph was also unaffected by metformin. Assessment of the microbiome showed that the larvae microbiome was dominant in Proteobacteria and Firmicutes, with small increases in Proteobacteria and decreases in Firmicutes with increasing exposure to metformin. No treatment effects were found for microbiome diversity measures. Control fish euthanized with the anesthetic tricaine methane sulfonate had decreased alpha diversity compared to those sampled by spinal severance. This experiment demonstrates that metformin at environmentally relevant concentrations (3.44 and 33.6 μg/L) and at 10 times MWWE concentrations (269 µg/L) does not adversely affect larval growth or gut microbiome in this ubiquitous freshwater fish species.

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Section: Discussionmentioning

confidence: 99%

Chronic Embryo‐Larval Exposure of Fathead Minnows to the Pharmaceutical Drug Metformin: Survival, Growth, and Microbiome Responses

Parrott

Restivo

Kidd

et al. 2021

Enviro Toxic and Chemistry

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“…The alpha diversity of the gut microbiome in three-spined stickleback ( Gasterosteus aculeatus ) and European perch ( Perca fluviatilis ) is different between sex [ 16 ]. The significant separation is found in the microbial communities between male and female fathead minnow ( Pimephales promelas ) based on beta diversity metrics [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Composition and diversity of gut microbiota in Pomacea canaliculata in sexes and between developmental stages

Chen

Xiao

et al. 2021

BMC Microbiol

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Background The apple snail, Pomacea canaliculata, is one of the world’s 100 worst invasive alien species and vector of some pathogens relevant to human health. Methods On account of the importance of gut microbiota to the host animals, we compared the communities of the intestinal microbiota from P. canaliculata collected at different developmental stages (juvenile and adult) and different sexes by using high-throughput sequencing. Results The core bacteria phyla of P. canaliculata gut microbiota included Tenericutes (at an average relative abundance of 45.7 %), Firmicutes (27.85 %), Proteobacteria (11.86 %), Actinobacteria (4.45 %), and Cyanobacteria (3.61 %). The female group possessed the highest richness values, whereas the male group possessed the lowest bacterial richness and diversity compared with the female and juvenile group. Both the developmental stages and sexes had important effects on the composition of the intestinal microbiota of P. canaliculata. By LEfSe analysis, microbes from the phyla Proteobacteria and Actinobacteria were enriched in the female group, phylum Bacteroidetes was enriched in the male group, family Mycoplasmataceae and genus Leuconostoc were enriched in the juvenile group. PICRUSt analysis predicted twenty-four metabolic functions in all samples, including general function prediction, amino acid transport and metabolism, transcription, replication, recombination and repair, carbohydrate transport and metabolism, etc. Conclusions This study provided a general understanding of the diversity characteristics of intestinal microbial communities of P. canaliculata, and indicated that developmental stage and gender could both influence the intestinal microbes of P. canaliculata. Further study may focus on the interaction between the gut microbiota and their host.

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“…For example, Rhodobacteraceae were found in the gut of a Ciona species, suggesting that the occultatumvirus EVGs found in Ciona robusta could also infect bacteria in this family. Similarly, Rhodobacteraceae and Hyphomicrobiales were found in the gut or skin of similar fish species from which occultatumvirus EVGs were retrieved (DeBofsky et al 2020; Nielsen et al 2018; Tarnecki et al 2022). In the same vein, the tortoise Gopherus morafkai harbors in its nasal microbiome both Hyphomicrobiales and Rhodobacterales (Weitzman et al 2018) , making members of the two orders very likely the hosts of the occultatumviruses found in the fecal viromes from similar tortoises.…”

Section: Discussionmentioning

confidence: 94%

New Microviridae isolated from Sulfitobacter reveals two cosmopolitan subfamilies of ssDNA phages infecting marine and terrestrial Alphaproteobacteria

Zucker

Bischoff

Ndela

et al. 2022

Preprint

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The Microviridae family represents one of the major clades of ssDNA phages. Their cultivated members are lytic and infect Proteobacteria, Bacteroidetes, and Chlamydiae. Prophages have been predicted in genomes from Bacteroidales, Hyphomicrobiales, and Enterobacteraceae and cluster within the "Alpavirinae", "Amoyvirinae" and Gokushovirinae. We have isolated "Ascunsovirus oldenburgi" ICBM5, a novel phage distantly related to known Microviridae. It infects Sulfitobacter dubius SH24-1b and uses both a lytic and a carrier-state life strategy. Using ICBM5 proteins as a query, we uncovered in publicly available resources 65 new microviridae prophages and episomes in bacterial genomes and retrieved 47 environmental viral genomes (EVGs) from various viromes. Genome clustering based on protein content and phylogenetic analysis showed that ICBM5, together with Rhizobium phages, new prophages, episomes, and EVGs cluster within two new phylogenetic clades, here tentatively assigned the rank of subfamily and named "Tainavirinae" and "Occultatumvirinae". They both infect Rhodobacterales. Occultatumviruses also infect Hyphomicrobiales, including nitrogen-fixing endosymbionts from cosmopolitan legumes. A biogeographical assessment showed that tainaviruses and occultatumviruses are spread worldwide, in terrestrial and marine environments. The new phage isolated here shed light onto new and diverse branches of the Microviridae tree, suggesting that much of the ssDNA phage diversity remains in the dark.

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Differential responses of gut microbiota of male and female fathead minnow (Pimephales promelas) to a short-term environmentally-relevant, aqueous exposure to benzo[a]pyrene

Cited by 38 publications

References 66 publications

Chronic Embryo‐Larval Exposure of Fathead Minnows to the Pharmaceutical Drug Metformin: Survival, Growth, and Microbiome Responses

Chronic Embryo‐Larval Exposure of Fathead Minnows to the Pharmaceutical Drug Metformin: Survival, Growth, and Microbiome Responses

Composition and diversity of gut microbiota in Pomacea canaliculata in sexes and between developmental stages

New Microviridae isolated from Sulfitobacter reveals two cosmopolitan subfamilies of ssDNA phages infecting marine and terrestrial Alphaproteobacteria

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