Composition and diversity of gut microbiota in Pomacea canaliculata in sexes and between developmental stages

Chen, Lian; Li, Shuxian; Xiao, Qin; Lin, Ying; Li, Xuexia; Qu, Yan‐Fu; Wu, Guogan; Li, Hong

doi:10.1186/s12866-021-02259-2

Cited by 20 publications

(29 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To some extent, this is consistent with the view that tadpoles have a diverse diet (Bennett, 1992;Karasov, 1992). The functional differences of intestinal microbiota at different developmental stages might be attributed to the changes of diet and development (Chen, Cui, et al, 2021;Chen, Li, et al, 2021;Moya & Ferrer, 2016). For example, the relative abundance of Clostridiaceae was more enriched in the forelimb bud stage, the infections with bacteria of the genus Sarcina (family Clostridiaceae) in human and animal were associated with gastric dilation and emphysematous gastritis (Owens et al, 2021), consistent with the functional prediction that human disease were more enriched in the forelimb bud stage.…”

Section: Discussionsupporting

confidence: 82%

“…Therefore, it is necessary to expand intestinal microbiota studies with more species. Hynobius maoershanensis primarily lives in the surrounding area of the alpine swamp and the vegetation nearby consists primarily of hemlock forest and mountaintop dwarf forest (Chen, Cui, et al, 2021;Chen, Li, et al, 2021;Jiang & Jiang, 2006). The larvae of H. maoershanensis live an aquatic life, whereas the adults live amphibiously.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Variation in the intestinal microbiota at different developmental stages of Hynobius maoershanensis

Yang

Cui

Ning

et al. 2022

Ecology and Evolution

View full text Add to dashboard Cite

Intestinal microbiota play an important role in the life of amphibians and its composition may vary by developmental stage. In this study, 16S rRNA high‐throughput sequencing was used to profile the intestinal microbiota of Hynobius maoershanensis, which exclusively inhabit the Maoer Mountain swamp at an altitude of approximately 2,000 m. We characterized the bacterial composition, structure, and function of the microbiota of H. maoershanensis at different developmental stages. The alpha diversity was not markedly different for the Simpson, Shannon, Ace, and Sobs indices of microbes. The beta diversity revealed that there were age‐related differences in the structure of the intestinal microbes of H. maoershanensis, specifically, at the phylum level. Bacteroidetes and Proteobacteria were the dominant bacteria present in the adult stage, and the relative abundance of Bacteroidetes was significantly higher compared with that of tadpoles. Firmicutes and Proteobacteria were the dominant phylum during the tadpole stage and their relative abundance was significantly higher compared with the adult period. Functional analysis revealed that the pathways associated with organismal systems and metabolism were significantly enriched in the adults, whereas human diseases, genetic information processing, and cellular processes were more enriched in the hindlimb bud stage. Human diseases and environmental information processing were more enriched in the forelimb bud stage at KEGG pathway level 1. Possibilities for the observed discrepancies include the adaptation to eating habits and the remodeling of the intestines during development. We speculated that H. maoershanensis adults may be more suitable to a high‐fiber diet, whereas the tadpoles are associated with a carnivorous diet. Our study provides evidence of variations in the intestinal microbiota during development in amphibians, highlighting the influence of historical developments on the intestinal microbiota and an increased understanding of the importance of physiological characteristics in shaping the intestinal microbiota of amphibians. These data will help us formulate more effective protection measures for H. maoershanensis.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Variation in the intestinal microbiota at different developmental stages of Hynobius maoershanensis

Yang

Cui

Ning

et al. 2022

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…Growing evidence indicated that the gut microbiota was a dynamic system that was inevitably influenced by multiple intrinsic and extrinsic factors, including diet, age, and sex environment (32)(33)(34). Generally, the physiological fluctuations of gut microbiota caused by the above-mentioned factors cannot affect intestinal normal function and homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Integrated Bacteria-Fungi Diversity Analysis Reveals the Gut Microbial Changes in Buffalo With Mastitis

Chen

Zhang

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

The gut microbial community is closely related to mastitis, but studies regarding the influences of mastitis on gut microbiota in buffalo remain scarce. Herein, we characterized the differences in gut bacterial and fungal communities between mastitis-affected and healthy buffalos. Interestingly, although mastitis had no effect on gut bacterial and fungal diversities in the buffalos, some bacterial and fungal taxa were significantly altered. Bacterial and fungal taxonomic analysis showed that the preponderant bacterial phyla (Firmicutes and Bacteroidetes) and fungal phyla (Ascomycota and Basidiomycota) in buffalo were the same regardless of health status. At the level of genus, the changes in some gut bacterial and fungal abundances between both groups were gradually observed. Compared with healthy buffalos, the proportions of 3 bacterial genera (uncultured_bacterium_f_Muribaculaceae, Eubacterium_nodatum_group, and Lachnoclostridium_10) and 1 fungal genus (Pichia) in the mastitis-affected buffalo were significantly increased, whereas 4 bacterial genera (Ruminococcus_2, Candidatus_Stoquefichus, Turicibacter, and Cellulosilyticum) and 4 fungal genera (Cladosporium, Thermothelomyces, Ganoderma and Aspergillus) were significantly decreased. Taken together, this research revealed that there was significant difference in the compositions of the gut microbial community between the healthy and mastitis-affected buffalos. To our knowledge, this is the first insight into the characteristics of the gut microbiota in buffalos with mastitis, which is beneficial to understand the gut microbial information of buffalo in different health states and elucidate the pathogenesis of mastitis from the gut microbial perspective.

show abstract

“…P. canaliculata in winter were generally sampled under the soil by fishing net because snails overwinter were buried in the bottom sediments. All experimental individuals were wiped by 75% ethanol three times, followed by rinsing twice in distilled water to sanitize the surface prior to dissection and removing the shell from each snail (Chen et al, 2021 ). Coiled gut contents were extracted carefully to avoid rupturing the gut wall.…”

Section: Methodsmentioning

confidence: 99%

“…The composition of the microbiota was diverse among the different gut sections. Significant differences were found in the structure of gut microbiota among female, male, and juvenile groups, suggesting the gut microbiota of P. canaliculata has been affected by the developmental stages (Chen et al, 2021 ). Zhou et al ( 2022 ) investigated difference of gut microbiota between P. canaliculata and native snail Cipangopaludina chinensis .…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation in structure and function of gut microbiota in Pomacea canaliculata

Qian

Yang

et al. 2022

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

Gut microbiota is associated with host health and its environmental adaption, influenced by seasonal variation. Pomacea canaliculata is one of the world's 100 worst invasive alien species. Here, we used high‐throughput sequencing of the 16S rRNA gene to analyze the seasonal variation of gut microbiota of P. canaliculata . The results suggested that the predominant gut microbial phyla of P. canaliculata included Firmicutes and Proteobacteria, which helped digest plant food and accumulate energy. The gut microbiota of P. canaliculata in summer group showed the highest diversity, whereas the winter group possessed the lowest, probably due to the shortage of food resources of P. canaliculata in winter. Principal coordinate analysis analysis based on unweighted unifrac and weighted unifrac indicated that the composition of gut microbiota of P. canaliculata significantly varied across seasons. Bacteroidetes tended to be enriched in summer by linear discriminant analysis effect size analysis. Actinobacteria and Cyanobacteria were extremely abundant in autumn, while Fusobacteria and Cetobacterium enriched in winter. In conclusion, the structure of the gut microbiota of P. canaliculata was significantly different among seasons, which was beneficial to the environment adaptation and the digestion and metabolism of food during different periods.

show abstract

Composition and diversity of gut microbiota in Pomacea canaliculata in sexes and between developmental stages

Cited by 20 publications

References 68 publications

Variation in the intestinal microbiota at different developmental stages of Hynobius maoershanensis

Variation in the intestinal microbiota at different developmental stages of Hynobius maoershanensis

Integrated Bacteria-Fungi Diversity Analysis Reveals the Gut Microbial Changes in Buffalo With Mastitis

Seasonal variation in structure and function of gut microbiota in Pomacea canaliculata

Contact Info

Product

Resources

About