Differential requirement of EGFR signaling for the expression of defective proventriculus gene in the Drosophila endoderm and ectoderm

Shirai, Tetsuya; Maehara, Atsushi; Kiritooshi, Naruto; Matsuzaki, Fumio; Handa, Hiroshi; Nakagoshi, Hideki

doi:10.1016/j.bbrc.2003.10.017

Cited by 10 publications

(6 citation statements)

References 20 publications

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“…We used an antibody raised against the carboxy-terminal region of DVE, which contains both homeodomains, to analyse DVE expression in the developing eye-antennal disc. This antibody has previously been used to study DVE in embryonic development and in the wing imaginal disc (Nakagoshi et al 1998;Nakagoshi et al 2002;Kolzer et al 2003;Shirai et al 2003). DVE protein expression in late third instar eye-antennal discs mimics the 10399/11037 enhancer trap pattern with strong expression in the dorsal head primordium, along the medial edge of the antennal disc and in distal antennal segments (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…dve is a member of the cut superclass of homeobox genes and contains two divergent carboxyterminal homeodomains and an amino-terminal COM-PASS domain (Nakagoshi et al 1998;Fuss and Hoch 1998;Bürglin and Cassatta 2002). It is essential for the development of the larval gut (Nakagoshi et al 1998;Fuss and Hoch 1998;Shirai et al 2003), and hypomorphic dve mutants that survive to adulthood also have defects in the wing, head, legs and halteres (Nakagoshi et al 2002;Kolzer et al 2003). We used an antibody raised against the carboxy-terminal region of DVE, which contains both homeodomains, to analyse DVE expression in the developing eye-antennal disc.…”

Section: Resultsmentioning

confidence: 99%

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Expression of defective proventriculus during head capsule development is conserved in Drosophila and stalk-eyed flies (Diopsidae)

et al. 2005

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Hypercephaly, in the form of lateral extensions of the head capsule, is observed in several families of Diptera. A particularly exaggerated form is found in Diopsid stalk-eyed flies, in which both eyes and antennae are laterally displaced at the end of stalks. The processes of early development and specification of the head capsule in stalk-eyed flies are similar to those in Drosophila melanogaster. In Drosophila the homeobox gene ocelliless (oc) shows a mediolateral gradient of expression across the region of the eye-antennal imaginal disc that gives rise to the head capsule and specifies the development of different head structures. The genes and developmental mechanisms that subsequently define head shape in Drosophila and produce hypercephaly in stalk-eyed flies remain unclear. To address this, we performed an enhancer trap screen for Drosophila genes expressed in the same region as oc and identified the homeobox gene defective proventriculus (dve). In the eye-antennal imaginal disc, dve is coexpressed with oc in the region that gives rise to the head capsule and is active along the medial edge of the antennal disc and in the first antennal segment. Analyses of dve expression in mutant eye-antennal discs are consistent with it acting downstream of oc in the development of the head capsule. We confirm that orthologues of dve are present in a diverse panel of five stalk-eyed fly species and analyse patterns of dve sequence variation within the clade. Our results indicate that dve expression and sequence are both highly conserved in stalk-eyed flies.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Expression of defective proventriculus during head capsule development is conserved in Drosophila and stalk-eyed flies (Diopsidae)

et al. 2005

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“…This gene owes its name to studies in D. melanogaster showing that mutants for dve have morphologic defects of the proventriculus [144]–[146], a region that develops at the junction of the foregut and the midgut and functions as a valve regulating the passage of food. During development, dve has been shown to respond to Wg (Wnt), Dpp (BMP), EGFR, and Notch signaling in the gut [144], [146], [147]; however, dve also has an important role in the digestive physiology of the gut. Expression of dve in midgut copper cells—cells that resemble absorptive mouse enterocyte cells—is necessary for acid secretion and for copper absorption [148].…”

Section: Resultsmentioning

confidence: 99%

An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus

et al. 2014

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The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas' disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as pro-enzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet.

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“…Expression of dve as well as lab mainly depends on Dpp signaling and also requires epidermal growth factor receptor (EGFR) signaling mediated by Vn in the middle midgut (Szüts et al . 1998; Shirai et al . 2003).…”

Section: Functional Development Of Copper Cellsmentioning

confidence: 99%

Functional specification in the Drosophila endoderm

Nakagoshi

2005

Dev Growth Differ

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The discovery of homeobox gene clusters led us to realize that the mechanisms for body patterning and other developmental programs are evolutionally-conserved in vertebrates and invertebrates. The endoderm contributes to the lining of the gut and associated organs such as the liver and pancreas, which are critical for physiological functions. Our knowledge of endoderm development is limited; however, recent studies suggest that cooperation between the HNF3/Fork head and GATA transcription factors is crucial for endoderm specification. It is necessary to further understand the mechanism through which cells become functionally organized. Molecular genetic analyses of the Drosophila endoderm would provide insights into this issue. During proventriculus morphogenesis, a simple epithelial tube is folded into a functional multilayered structure, while two functions of midgut copper cells (i.e. copper absorption and acid secretion) can be easily visualized. The homeobox gene defective proventriculus (dve) plays key roles in these functional specifications.

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Differential requirement of EGFR signaling for the expression of defective proventriculus gene in the Drosophila endoderm and ectoderm

Cited by 10 publications

References 20 publications

Expression of defective proventriculus during head capsule development is conserved in Drosophila and stalk-eyed flies (Diopsidae)

Expression of defective proventriculus during head capsule development is conserved in Drosophila and stalk-eyed flies (Diopsidae)

An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus

Functional specification in the Drosophila endoderm

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