Differential pulse voltammetric ochratoxin A assay based on the use of an aptamer and hybridization chain reaction

Qing, Ying; Li, Xuan; Chen, Shuai; Zhou, Xipeng; Luo, Ming; Xu, Xuan; Li, ChaoRui; Qiu, Jingfu

doi:10.1007/s00604-017-2080-z

Cited by 37 publications

(11 citation statements)

References 34 publications

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“…The analytical performance of the electrochemical aptasensor was compared with published studies on the electrochemical detection of OTA, as shown in Table S4. Significantly, the current approach provides a lower LOD and wider working range compared to other silver metallization assays. − The LOD of our system is marginally higher than the platforms presented in refs , ; however, it is important to note that the linear range in the current system is much wider and the method far simpler to implement due to the use of disposable SPGEs.…”

Section: Resultsmentioning

confidence: 84%

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

et al. 2019

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Ochratoxin A (OTA)a mycotoxin produced by Aspergillus and Penicillium fungiis a carcinogen and common trace contaminant in agricultural and processed food products. As consumption is detrimental to human and animal health, regular product monitoring is vital, and highly sensitive and portable OTA sensors are necessary in many circumstances. Herein, we report an ultrasensitive, electroanalytical aptasensor for precise determination of OTA at trace levels. The sensor leverages a DNA aptamer to capture OTA and silver metallization as a signal enhancer. Exonuclease I is used to digest unbound aptamers, engendering excellent background signal suppression and sensitivity enhancements. Efficient optimization of assay conditions is achieved using central composite design (CCD), allowing rapid evaluation of both the electrode and square wave voltammetry parameter space. The sensor exhibits excellent analytical performance, with a concentration limit of detection of 0.7 pg mL −1 , a limit of quantitation of 2.48 pg mL −1 , and a linear dynamic range (R 2 = 0.968) of over 6 orders of magnitude (between 1 pg mL −1 and 0.1 μg mL −1 ). Direct comparison with ultraperformance liquid chromatography (UPLC) indicates excellent analytical performance for standard solutions (R 2 = 0.995) and spiked beer samples (R 2 = 0.993), with almost quantitative recovery and less than 5% relative standard deviation (RSD).

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Section: Resultsmentioning

confidence: 84%

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

et al. 2019

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“…For the determination of the mycotoxin OTA, a differential pulse voltammetric aptasensor based on hybridization chain reaction (HCR) was developed. The assay was successfully applied to the determination of OTA in cereal samples with a detection limit of 2 pg/mL [ 131 ]. A portable and reusable optofluidic immunosensor OIP-v2 was developed for rapid and sensitive on-site detection of DON using DON-BSA modified bio-probes as biorecognition elements.…”

Section: Instrumental Analysismentioning

confidence: 99%

Recent Advances in Mycotoxin Determination in Fish Feed Ingredients

et al. 2023

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Low-cost plant-based sources used in aquaculture diets are prone to the occurrence of animal feed contaminants, which may in certain conditions affect the quality and safety of aquafeeds. Mycotoxins, a toxic group of small organic molecules produced by fungi, comprise a frequently occurring plant-based feed contaminant in aquafeeds. Mycotoxin contamination can potentially cause significant mortality, reduced productivity, and higher disease susceptibility; thus, its timely detection is crucial to the aquaculture industry. The present review summarizes the methodological advances, developed mainly during the past decade, related to mycotoxin detection in aquafeed ingredients, namely analytical, chromatographic, and immunological methodologies, as well as the use of biosensors and spectroscopic methods which are becoming more prevalent. Rapid and accurate mycotoxin detection is and will continue to be crucial to the food industry, animal production, and the environment, resulting in further improvements and developments in mycotoxin detection techniques.

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“…Therefore, the application of magnetic Fe 3 O 4 nanomaterials can significantly improve the sensitivity and selectivity of detection assays. 41 Common Apt-based FL signal amplification strategies mainly include rolling circle amplification (RCA), 42,43 polymerase chain reaction (PCR), 44 hybridization chain reaction(HCR), 28,45,46 strand displacement amplification (SDA), 47 and so forth comparing with RCA, PCR, and HCR, SDA does not require a specific temperature program to react and is an in vitro isothermal amplification technique. The DNA polymerization reaction of SDA involves the continuous replication of one strand of a DNA double-strand using a polymerase.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of Ochratoxin A

Guo

Wang

et al. 2023

ACS Omega

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Ochratoxin A (OTA) is a common mycotoxin, and it is a significant threat to human health throughout the food chain. In this study, a sensitive and specific fluorescent sensor based on magnetic separation technology combined with chain displacement amplification was developed for fast and easy detection of OTA in food. The designed strand displacement amplification can improve the sensitivity for the detection, and the magnetic nanomaterials can provide a large surface area, thus enhancing the capture efficiency of the target from the sample. Based on those designs, the experimental results showed that the proposed method displayed excellent performance. The linearity range was 0.5–128.0 ng/mL. The detection limit was 0.125 ng/mL; the relative standard deviations were 3.92–7.71%. Additionally, the developed method was satisfactorily applied to determine OTA in wheat, corn, and red wine samples at three spiked levels (1.0, 8.0, and 64.0 ng/mL). The recoveries ranged from 85.45 to 107.8% for wheat flour, 101.34 to 108.35% for corn flour, and 91.15 to 93.80% for red wine, respectively. Compared with high-performance liquid chromatography, the proposed method showed a lower limit of detection and equal recovery. Hence, the designed method is a potential and good detecting tool for OTA residue analysis in complex matrix samples.

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Differential pulse voltammetric ochratoxin A assay based on the use of an aptamer and hybridization chain reaction

Cited by 37 publications

References 34 publications

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

Recent Advances in Mycotoxin Determination in Fish Feed Ingredients

Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of Ochratoxin A

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