Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of Ochratoxin A

Abstract

Ochratoxin A (OTA) is a common mycotoxin, and it is a significant threat to human health throughout the food chain. In this study, a sensitive and specific fluorescent sensor based on magnetic separation technology combined with chain displacement amplification was developed for fast and easy detection of OTA in food. The designed strand displacement amplification can improve the sensitivity for the detection, and the magnetic nanomaterials can provide a large surface area, thus enhancing the capture efficiency of the target from the sample. Based on those designs, the experimental results showed that the proposed method displayed excellent performance. The linearity range was 0.5–128.0 ng/mL. The detection limit was 0.125 ng/mL; the relative standard deviations were 3.92–7.71%. Additionally, the developed method was satisfactorily applied to determine OTA in wheat, corn, and red wine samples at three spiked levels (1.0, 8.0, and 64.0 ng/mL). The recoveries ranged from 85.45 to 107.8% for wheat flour, 101.34 to 108.35% for corn flour, and 91.15 to 93.80% for red wine, respectively. Compared with high-performance liquid chromatography, the proposed method showed a lower limit of detection and equal recovery. Hence, the designed method is a potential and good detecting tool for OTA residue analysis in complex matrix samples.

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

“…Enzyme-free catalytic hairpin assembly (CHA) reaction or enzyme-driven oligonucleotides cycling seems to be a promising technique to achieve signal amplification (Chen, Zhang et al, 2021;Wu et al, 2023;Zhang, Wang et al, 2020;Zhao et al, 2023). In the work of Chen et al, the immunocomplexes of OTA antigen-MNPs/mAb-barcode DNA-AuNPs can be dissociated by the addition of dithiothreitol; con-sequently, the disassembled barcode DNAs triggered the CHA reaction, thereby substantially enhancing the signal amplification (Figure 6e) (Chen et al, 2020).…”