Differential proteome analysis of the cell differentiation regulated by BCC, CRH, CXCR4, GnRH, GPCR, IL1 signaling pathways in Chinese fire-bellied newt limb regeneration

Geng, Xiaofang; Xu, Tiantian; Niu, Zhipeng; Zhou, Xiaochun; Zhao, Liang; Xie, Zhenrong; Xue, Deming; Zhang, Fuchun; Xu, Chi

doi:10.1016/j.diff.2014.10.002

Cited by 16 publications

(13 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In brief, Cy2-, Cy3-, Cy5-labeled samples were mixed together and dissolved in rehydration buffer (7 M urea, 2 M thiourea, 2% CHAPS, 18 mM DTT, 2% IPG buffer 3-10 NL). The isoelectric focusing (IEF) was performed in Ettan IPGphor (GE Healthcare, USA) in accordance with the method previously published (Geng et al, 2014). After IEF, the gel strip was equilibrated in equilibration buffer containing 1% DTT for 15 min and then alkylated in alkylation equilibration buffer containing 2.5% iodoacetamide for 15 min.…”

Section: Two-dimensional Fluorescence Difference Gel Electrophoresis mentioning

confidence: 99%

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Sun

Geng

Guo

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

Self Cite

View full text Add to dashboard Cite

Section: Two-dimensional Fluorescence Difference Gel Electrophoresis mentioning

confidence: 99%

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Sun

Geng

Guo

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

Self Cite

View full text Add to dashboard Cite

“…Then, IPA software was utilized to analyze the heatmap of biofunctions based on the expression changes of each branch-related genes using comparison analysis (Geng et al, 2014). Briefly, the differentially expressed each branch-related gene symbols and their corresponding expression extrema in eight liver cell types were uploaded to the "Dataset Files" of IPA.…”

Section: Function Prediction Of Every Branch Of Opn-mediated Signalinmentioning

confidence: 99%

Expression profiles uncover the correlation of OPN signaling pathways with rat liver regeneration at cellular level

Wang

Chen

et al. 2015

Cell Biology International

Self Cite

View full text Add to dashboard Cite

Osteopontin (OPN) could participate in the occurrence of multiple liver diseases via promoting inflammation, cell activation, proliferation, and migration. However, the correlation of OPN with liver regeneration (LR) is poorly defined. Previous studies from us and others revealed that OPN was probably involved in the activation and proliferation of various hepatic cell types during LR. In this study, to further investigate the underlined mechanism of OPN in regulating LR, eight hepatic cell types were isolated and purified from rat regenerative livers at 10 time points. The gene expression profiles of above hepatic cells were assayed by Rat Genome 230 2.0 chips, and then IPA software was used to uncover the correlations of gene expression changes with physiological activities. The findings demonstrated that the majority of the OPN pathway-related genes were up-regulated in hepatocytes (HCs), pit cells (PCs), oval cells (OCs), and biliary epithelial cells (BECs) but down-regulated in other four cell types including sinusoidal endothelial cells (SECs), Kupffer cells (KCs), dendritic cells (DCs), and hepatic stellate cells (HSCs). Thereafter, functional enriched analysis by IPA indicated that OPN signaling pathway might promote cell proliferation, activation, migration, and inflammation in HCs, OCs, BECs, and PCs, and slightly boost proliferation and migration of SECs and KCs but inhibit inflammation response and chemotaxis in SECs and KCs and almost all physiological processes in DCs and HSCs. Morever, apoptosis, cell death, and necrosis were remarkably inhibited through JAK/STAT, ERK1/2, and NF-kB branches in almost every cell type. These above results suggest that OPN signaling pathway is closely related to HCs, OCs, BECs, and PCs but has less regulatory effect on SECs, KCs, HSCs, and DCs during rat LR.

show abstract

“…Protein extraction was performed according to the method as previously described (Geng et al, 2014). Briefly, the liver tissues were ground into fine powder with liquid nitrogen, and then suspended in lysis buffer.…”

Section: Protein Extractionmentioning

confidence: 99%

Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. The Chinese fire-bellied newt, Cynops orientalis, belonging to Amphibia, Caudata, Salamandridae is a species endemic to China. The liver, which is an important digestive gland and the largest amphibian organ, has various functions, including detoxification, glycogen storage, protein synthesis, and hormone production. However, the newt liver has rarely been studied at the molecular level. We performed histomorphology and high-throughput proteomic analysis of the Chinese fire-bellied newt liver, using hematoxylin and eosin (H&E) staining and two-dimensional electrophoresis coupled with mass spectrometry. The H&E staining showed that the newt liver nuclei are large and round, are located in the lateral cytoplasm, and contain a large quantity of lipid droplets. Melanins were abundantly present throughout the hepatic parenchyma. The proteome analysis showed a total of 545 proteins detected in the newt liver. Furthermore, a gene ontology analysis suggested that these proteins were associated with metabolism, immune response, cellular homeostasis, etc. Among these, proteins with metabolic functions were found to be the most abundant and highly expressed. This supports the role of the liver as the metabolic center. The proteomic results provide new insights into the aspects of the liver proteomes of the Chinese fire-bellied newt. The identification of a more global liver proteome in the newt may provide a basis for characterizing and comparing the liver proteomes from other amphibian species.

show abstract

Differential proteome analysis of the cell differentiation regulated by BCC, CRH, CXCR4, GnRH, GPCR, IL1 signaling pathways in Chinese fire-bellied newt limb regeneration

Cited by 16 publications

References 52 publications

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander

Expression profiles uncover the correlation of OPN signaling pathways with rat liver regeneration at cellular level

Proteome analysis of the liver in the Chinese fire-bellied newt Cynops orientalis

Contact Info

Product

Resources

About