Differential protein expression in patients with urosepsis

Abstract

PurposeUrosepsis in adults comprises approximately 25% of all sepsis cases, and is due to complicated urinary tract infections in most cases. However, its mechanism is not fully clarified. Urosepsis is a very complicated disease with no effective strategy for early diagnosis and treatment. This study aimed to identify possible target-related proteins involved in urosepsis using proteomics and establish possible networks using bioinformatics.MethodsFifty patients admitted to the Urology Unit of Lanzhou General PLA (Lanzhou, China), from October 2012 to October 2015, were enrolled in this study. The patients were further divided into shock and matched-pair non-shock groups. 2-DE technique, mass spectrometry and database search were used to detect differentially expressed proteins in serum from the two groups.ResultsSix proteins were found at higher levels in the shock group compared with non-shock individuals, including serum amyloid A-1 protein (SAA1), apolipoprotein L1 (APOL1), ceruloplasmin (CP), haptoglobin (HP), antithrombin-III (SERPINC1) and prothrombin (F2), while three proteins showed lower levels, including serotransferrin (TF), transthyretin (TTR) and alpha-2-macroglobulin (A2M).ConclusionNine proteins were differentially expressed between uroseptic patients (non-shock groups) and severe uroseptic patients (shock groups), compared with non-shock groups, serum SAA1, APOL1,CP, HP, SERPINC1and F2 at higher levels, while TF, TTR and A2M at lower levels in shock groups.these proteins were mainly involved in platelet activation, signaling and aggregation, acute phase protein pathway, lipid homeostasis, and iron ion transport, deserve further research as potential candidates for early diagnosis and treatment. (The conclusion seems too simple and vague, please re-write it. You may focus at what proteins have been expressed and introduce more detail about its significance.)

Combination of HBA1, TTR, and SERPINF2 in plasma defines phenotype correlated with severe burn outcome

“… 22 However, in urogenital sepsis, TTR within 24 h was lower in the shock group. 23 TTR has been found to be associated with mortality and protein energy malnutrition in ICU admissions, in that TTR in blood reflects protein catabolic losses in critically ill patients. 24 TTR reduction increases the generation of reactive oxygen species and induces apoptosis, which is associated with acute kidney injury.…”

Pathways Associated With Positive Sepsis Survival Outcomes in African American/Black and Non-Hispanic White Patients With Urinary Tract Infection

“…A prior proteomic study in UTI models and patients who developed sepsis proposed lipopolysaccharide-binding protein, clusterin, and VCAM1 as diagnostic markers of sepsis (52). Others have identified differentially expressed proteins involved in coagulation, the acute phase response, lipid homeostasis, and iron ion transport as associated with sepsis severity (53). Our laboratory previously used plasma proteomics to study sepsis survival in a diverse cohort of patients with primary intra-abdominal infection, identifying proteins related to hepatic fibrosis/hepatic stellate cell activation (e.g., VCAM1) that were decreased in sepsis survivors (39).…”

DIA-Based Proteomic Analysis of Plasma Protein Profiles in Patients with Severe Acute Pancreatitis

“…The results of the present study indicated that the levels of TF, CD5L, and LTF, the proteins involved in the inflammatory response, were decreased in plasma. Among them, TF, an N-glycosylated glycoprotein in the acute stage of AP, has been shown to be at a lower level during sepsis [13]; therefore, it was speculated that changes in TF could reflect the intensity of the inflammatory response [14]. CD5L, also known as Sp alpha, is a soluble protein that belongs to the B group of the scavenger receptor-rich cysteine superfamily secreted by macrophages and has been shown to promote the survival of macrophages [15,16].…”