Differential properties of human stromal cells from bone marrow, adipose, liver and cardiac tissues

Kellner, Joshua; Sivajothi, Santhosh; McNiece, Ian

doi:10.1016/j.jcyt.2015.07.009

Cited by 16 publications

(16 citation statements)

References 41 publications

(24 reference statements)

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“…The results showed that T/A‐mediated hBMSC‐derived hepatic cells could metabolize around 10 times more urea than the liver‐like cells in previous study (250 pg/cell/h vs 20 pg/cell/h) but secreted less albumin (~5 µg/mL/day) into medium compared with the hepatic cells differentiated from human adipose tissues (~30 µg/mL/day) and primary human hepatocytes (~70 µg/mL/day) . These results might reflect the fact that the sources of initial hMSCs could also play a key role for hepatic maturation, as pointed out by another study . Taken together, the results indicated that T/A‐mediated differentiation efficacy to direct hBMSCs into hepatic cell lineage could exhibit de novo liver physiology.…”

Section: Resultsmentioning

confidence: 73%

Efficient programming of human mesenchymal stem cell‐derived hepatocytes by epigenetic regulations

Tsai¹,

Yeh

Tsai³

et al. 2017

J of Gastro and Hepatol

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Section: Resultsmentioning

confidence: 73%

Efficient programming of human mesenchymal stem cell‐derived hepatocytes by epigenetic regulations

Tsai¹,

Yeh

Tsai³

et al. 2017

J of Gastro and Hepatol

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“…Bio-Oss consists of small granules, with a diameter of 10–60 nm. In addition, its physical structure is similar to that of human bone, and can be absorbed during bone regeneration (30). As angiogenesis and osteogenesis are tightly coupled during bone regeneration, the combination of HIF-1α and Bio-Oss appears to be an optimal choice for alveolar ridge preservation following tooth extraction.…”

Section: Discussionmentioning

confidence: 99%

Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

et al. 2019

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Hypoxia-inducible factor (HIF)-1α protein, which is upregulated by hypoxia, serves an important role in angiogenesis during osteogenesis. The aim of the present study was to investigate the effect of HIF-1α on alveolar ridge preservation in a dog tooth extraction model. Six beagle dogs were used in the present study. The second and fourth premolar teeth of the lower jaws on both sides were extracted. Two unilateral extraction sockets were randomly selected and filled with Bio-Oss and Bio-Oss + HIF-1α. The contralateral sockets remained unfilled and served as the negative control. Micro-computed tomography examination and histological staining were performed to examine the difference of new bone formation among the three groups. Western blotting and reverse transcription-quantitative polymerase chain reaction analysis were used to detect the expression levels of osteogenesis- and angiogenesis-associated genes in the bone tissues of the three groups. Twelve weeks post-surgery, trabecular bone formation in the Bio-Oss + HIF-1α group was significantly increased compared with the other groups. The expression levels of osteogenesis-associated genes (runt-related transcription factor 2, osteoblast-specific transcription factor osterix and osteocalcin) and angiogenesis-associated genes (HIF-1α and vascular endothelial growth factor) were all significantly increased in the Bio-Oss + HIF-1α group compared with the other two groups (P<0.05). The present results indicated that Bio-Oss with HIF-1α can promote osteogenesis and angiogenesis in vivo and may be used as an effective treatment for the preservation of the alveolar ridge.

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“… 27 – 29 Local tissue derived MSCs, which share many features with other MSCs, can have additional special attributes that may be potentially useful for certain clinical applications. 30 – 32 For instance, Co-MSCs were shown to have antifibrotic effects and prevent the development of corneal scarring after injury. 1 …”

Section: Discussionmentioning

confidence: 99%

Corneal Mesenchymal Stromal Cells Are Directly Antiangiogenic via PEDF and sFLT-1

Eslani

Putra

Shen

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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PurposeTo evaluate the angiogenic properties of corneal derived mesenchymal stromal cells (Co-MSC).MethodsCo-MSCs were extracted from human cadaver, and wild-type (C57BL/6J) and SERPINF1−/− mice corneas. The MSC secretome was collected in a serum-free medium. Human umbilical vein endothelial cell (HUVEC) tube formation and fibrin gel bead assay (FIBA) sprout formation were used to assess the angiogenic properties of Co-MSC secretome. Complete corneal epithelial debridement was used to induce corneal neovascularization in wild-type mice. Co-MSCs embedded in fibrin gel was applied over the debrided cornea to evaluate the angiogenic effects of Co-MSCs in vivo. Immunoprecipitation was used to remove soluble fms-like tyrosine kinase-1 (sFLT-1) and pigment epithelium-derived factor (PEDF, SERPINF1 gene) from the Co-MSC secretome.ResultsCo-MSC secretome significantly inhibited HUVECs tube and sprout formation. Co-MSCs from different donors consistently contained high levels of antiangiogenic factors including sFLT-1 and PEDF; and low levels of the angiogenic factor VEGF-A. In vivo, application of Co-MSCs to mouse corneas after injury prevented the development of corneal neovascularization. Removing PEDF or sFLT-1 from the secretome significantly diminished the antiangiogenic effects of Co-MSCs. Co-MSCs isolated from SERPINF1−/− mice had significantly reduced antiangiogenic effects compared to SERPINF1+/+ (wild-type) Co-MSCs.ConclusionsThese results illustrate the direct antiangiogenic properties of Co-MSCs, the importance of sFLT-1 and PEDF, and their potential clinical application for preventing pathologic corneal neovascularization.

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Differential properties of human stromal cells from bone marrow, adipose, liver and cardiac tissues

Cited by 16 publications

References 41 publications

Efficient programming of human mesenchymal stem cell‐derived hepatocytes by epigenetic regulations

Efficient programming of human mesenchymal stem cell‐derived hepatocytes by epigenetic regulations

Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

Corneal Mesenchymal Stromal Cells Are Directly Antiangiogenic via PEDF and sFLT-1

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