Differential long-term expression and methylation of the hygromycin phosphotransferase (hph) and β-glucuronidase (GUS) genes in transgenic pearl millet (Pennisetum glaucum) callus

Lambé, Pascal; Dinant, Monique; Matagne, RenéF.

doi:10.1016/0168-9452(95)04124-d

Cited by 60 publications

(27 citation statements)

References 35 publications

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“…Dans toutes ces études, l'apex caulinaire n'est généralement pas l'explant utilisé et, lorsque c'est le cas (Lambé et al, 1995;, la régénération de plantes à partir des suspensions cellulaires n'a pas fait l'objet d'étude approfondie. Après avoir déterminé les conditions optimales d'établissement de suspensions cellulaires embryogènes (Tiécoura et al, 2014), ce travail, présente pour la première fois, et à partir de la variété NE (Nord Est) de Pennisetum glaucum de Côte d'Ivoire, les facteurs influençant la régénération de plantes fertiles à partir de ces suspensions cellulaires issues de cals d'apex caulinaires.…”

Section: Introductionunclassified

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Tiecoura¹,

Alla-N'Nan²,

Kouassi³

et al. 2015

Int. J. Bio. Chem. Sci

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RÉSUMÉCe travail a pour objectif d'établir les conditions optimales de régénération de plantes fertiles à partir de suspensions cellulaires embryogènes en vue de leur utilisation pour une transformation génétique par bombardements de protoplastes. Ce travail réalisé sur des variétés de Pennisetum glaucum de Côte d'Ivoire a permis de mettre en évidence les facteurs qui influent (taille et âge des cals, milieu de conditonnement, et âge de la suspension cellulaire) sur la régénération de plantes fertiles. Il ressort de cette étude que la taille des cals et les milieux de conditionnement utilisés n'ont aucun effet sur le taux de régénération de plantes fertiles. Cependant, l'âge de la suspension cellulaire obtenue à partir de cals embryogènes et l'âge du cal initiateur de la suspension cellulaire influent sur le taux de régénération. Le taux de plantes normales régénérées est élevé durant les deux premiers mois de culture de la suspension cellulaire initiée à partir des cals de 2-3 mois (60 à 70%). Les plantes régénérées présentent les mêmes caractéristiques de croissance végétative (taille, longueur de la chandelle, nombre de barres, longueur de l'épi) que les plantes témoins. Pour le rendement, les plantes régénérées portent moins de grains (412) que les plantes témoins (715).

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Section: Introductionunclassified

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Tiecoura¹,

Alla-N'Nan²,

Kouassi³

et al. 2015

Int. J. Bio. Chem. Sci

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“…However, no plants were regenerated. Lambé et al (1995) reported maintenance of hygromycin resistant transformed callus of Pearl millet for two years without loss of resistance phenotype, but plants were not regenerated.…”

Section: Selection and Plant Regenerationmentioning

confidence: 99%

“…Lambé et al (1995Lambé et al ( , 2000 Vasil and Vasil 1981a, b;Vasil 1983, 1984;Taylor andVasil 1995, 1996 Xu et al 1984;Rao et al 1988;Reddy et al 1988Reddy et al , 1990Kavi Kishor et al 1992;Zeatin, NAA Osuna-Avilla et al 1995;Vishnoi and Kothari 1996 Abbreviations: 2,4-D, 2,4-dichlorophenoxy acetic acid; 2,4,5-T, 2,4,5-trichlorophenoxy acetic acid; NAA, a-naphthalene acetic acid; BAP, 6-benzylamino purine; Kn, Kinetin; IAA, Indole-3-acetic acid; pCPA, para chlorophenoxy acetic acid; Pic, 4-amino-3,5-6-trichloropicolinic acid; TDZ, Thidiazuron; IBA, Indole-3-butyric acid; GA 3 , Gibberellic acid.…”

Section: Vectorsmentioning

confidence: 99%

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Applications of biotechnology for improvement of millet crops: Review of progress and future prospects

Kothari

Kumar

Vishnoi

et al. 2005

Plant Biotechnology

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“…The phenomenon of multiple copy integration is commonly encountered in particle-bombardment-mediated genetic transformation of plants [15][16][17][18]. In earlier studies, a variable number of incorporated transgene copies ranging between 1 and 20 were reported in diverse crop plants [19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Figure: 7 Extraction Of Total Rna and Rt-pcr Analysismentioning

confidence: 99%

In vitro Regeneration and Optimization of Conditions for transformation methods in Pearl millet, Pennisetum glaucum (L.)

Naravula¹,

Maheshwari²,

Naidu³

et al. 2016

IJCBS

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Pearl millet is a dual-purpose crop used for grain and fodder and is grown primarily in Asia and Africa, where it occupies some 27 million ha. It is capable of growing on some of the poorest soils in dry, hot regions of Africa and Asia, where, as a poor man's source of dietary energy, it sustains a large proportion of the populace. It is also grown in other countries where, under relatively more favorable conditions, it provides grain for bullocks, dairy cows, and poultry. Downy mildew caused by Sclerospora graminicola (Sacc.) J. Schroet. is the most widespread and destructive disease of pearl millet causing severe economic losses. New genes can be introduced into this plant through Agrobacterium mediated and bombardment genetic transformation for its genetic improvement, which is dependent on the availability of suitable in vitro techniques. An efficient regeneration system has been developed for in vitro culture of pearl millet (Pennisetum glaucum L.) from the immature inflorescence. High frequency callus and shoot regeneration was obtained on Murashige and Skoog nutrient agar medium supplemented with 2mg/l 2,4-D and 0.2 mg/l NAA, 2 mg/l Kinetin and 30 g/l sucrose. On transfer to soil, the regenerated plantlets survived and appeared to be morphologically similar to the normal seed-grown plants. Histological analysis revealed the de novo origin of shoots from embryogenic callus in in vitro cultured pearl millet. Parameters affecting transformation were optimized by assaying phosphinothricin resistance to transformed calli and basta test for these leaves of plantlets after transferring to pots. These tissues appear to be susceptible to Agrobacterium infection and Particle gun flow mediated transformation carrying pCAMBIA2300 with osmotin and chitinase double construct and pPUR with bar genes, as well as shoot multiplication. The embryogenic callus was found competent to take up the DNA, which was monitored by transient bar gene with GV2600 at 0.6 O.D for Agrobacterium infection and 1µg/µl plasmid DNA from E.coli for cobombardment was found to be compatible in giving transgenics.

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Differential long-term expression and methylation of the hygromycin phosphotransferase (hph) and β-glucuronidase (GUS) genes in transgenic pearl millet (Pennisetum glaucum) callus

Cited by 60 publications

References 35 publications

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Applications of biotechnology for improvement of millet crops: Review of progress and future prospects

In vitro Regeneration and Optimization of Conditions for transformation methods in Pearl millet, Pennisetum glaucum (L.)

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