Differential Gene Regulation in Human Versus Rodent Hepatocytes by Peroxisome Proliferator-activated Receptor (PPAR) α

Lawrence, Jeffry B.; Liu, Ying; Chen, Shiying; DeLuca, John G.; Berger, Joel P.; Umbenhauer, Diane R.; Moller, David E.; Zhou, Gaochao

doi:10.1074/jbc.m103306200

Cited by 171 publications

(45 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[17][18][19] Our results suggest that LFO suppresses abdominal fat accumulation by regulation of rate-limiting enzyme activities related to fatty acid synthesis and oxidation in the liver.…”

Section: Investigation Of the Anti-obesity Action Of Licorice Flavonomentioning

confidence: 66%

Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

Kamisoyama

Honda

Tominaga

et al. 2008

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Section: Investigation Of the Anti-obesity Action Of Licorice Flavonomentioning

confidence: 66%

Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

Kamisoyama

Honda

Tominaga

et al. 2008

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…It has been shown that activation of PPAR␣ leads to transcriptional up-regulation of the CYP4A genes, which are also constituents of the microsomal biotransformation system in both proliferating and nonproliferating species (Lawrence et al, 2001;Cheon et al, 2005). It has further been shown that some UGT isoforms (UGT1A9, UGT2B4) are PPAR␣ target genes (Barbier et al, 2003a,b).…”

Section: Discussionmentioning

confidence: 99%

Clofibrate Increases Hepatic Triiodothyronine (T₃)- and Thyroxine (T₄)-Glucuronosyltransferase Activities and Lowers Plasma T₃and T₄Concentrations in Pigs

Luci

Kluge²,

Hirche³

et al. 2006

Drug Metab Dispos

View full text Add to dashboard Cite

ABSTRACT:In rats, clofibrate acts as a microsomal enzyme inducer and disrupts the metabolism of thyroid hormones by increasing hepatic glucuronidation of thyroxine. Whether similar effects occur in the pig has not yet been investigated. This study was performed to investigate the effect of clofibrate treatment on metabolism of thyroid hormones in pigs. To this end, an experiment with 18 pigs, which were assigned to two groups, was performed. One group received a control diet, and the other group was fed the same diet supplemented with 5 g of clofibrate/kg for 28 days. Pigs treated with clofibrate had higher hepatic activities of T 3 -and T 4 -UDP glucuronosyltransferases (UGT) and lower concentrations of total and free T 4 and total T 3 in plasma than control pigs (P < 0.05). Weights and histology of the thyroid gland (epithelial height, follicle lumen diameter) did not differ between the two groups, but pigs treated with clofibrate had higher mRNA concentrations of various genes in the thyroid responsive to thyroid-stimulating hormone (TSH) such as TSH receptor, sodium iodine symporter, thyroid peroxidase, and cathepsin B than control pigs (P < 0.05). Pigs treated with clofibrate also had lower hepatic mRNA concentrations of proteins involved in plasma thyroid hormone transport [thyroxine-binding globulin (P < 0.10), transthyretin (P < 0.05), and albumin (P < 0.05)] and thyroid hormone receptor ␣ 1 (P < 0.05) than control pigs. In conclusion, this study shows that clofibrate treatment induces a strong activation of T 3 -and T 4 -UGT in pigs, leading to increased glucuronidation and markedly reduced plasma concentrations of these hormones, accompanied by a moderate stimulation of thyroid function.

show abstract

“…Several genes are known that are targets of PPAR␣ in mice but not in humans (30,31). To investigate whether expression of FIAF is under control of PPAR␣ in other species, rat hepatoma FAO cells were treated with the PPAR␣ agonist Wy14643 (Fig.…”

Section: Fiaf Expression Is Regulated By All Three Ppars-previousmentioning

confidence: 99%

The Direct Peroxisome Proliferator-activated Receptor Target Fasting-induced Adipose Factor (FIAF/PGAR/ANGPTL4) Is Present in Blood Plasma as a Truncated Protein That Is Increased by Fenofibrate Treatment

Mandard

Zandbergen

Tan

et al. 2004

Journal of Biological Chemistry

246

256

View full text Add to dashboard Cite

The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was previously identified as a novel adipocytokine that was up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. To further characterize FIAF, we studied regulation of FIAF mRNA and protein in liver and adipose cell lines as well as in human and mouse plasma. Expression of FIAF mRNA was up-regulated by peroxisome proliferator-activated receptor ␣ (PPAR␣) and PPAR␤/␦ agonists in rat and human hepatoma cell lines and by PPAR␥ and PPAR␤/␦ agonists in mouse and human adipocytes. Transactivation, chromatin immunoprecipitation, and gel shift experiments identified a functional PPAR response element within intron 3 of the FIAF gene. At the protein level, in human and mouse blood plasma, FIAF was found to be present both as the native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated FIAF, whereas in human white adipose tissue and SGBS adipocytes, only native FIAF could be detected. Interestingly, truncated FIAF was produced by human liver. Treatment with fenofibrate, a potent PPAR␣ agonist, markedly increased plasma levels of truncated FIAF, but not native FIAF, in humans. Levels of both truncated and native FIAF showed marked interindividual variation but were not associated with body mass index and were not influenced by prolonged semistarvation. Together, these data suggest that FIAF, similar to other adipocytokines such as adiponectin, may partially exert its function via a truncated form.

show abstract

Differential Gene Regulation in Human Versus Rodent Hepatocytes by Peroxisome Proliferator-activated Receptor (PPAR) α

Cited by 171 publications

References 36 publications

Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

Clofibrate Increases Hepatic Triiodothyronine (T₃)- and Thyroxine (T₄)-Glucuronosyltransferase Activities and Lowers Plasma T₃and T₄Concentrations in Pigs

The Direct Peroxisome Proliferator-activated Receptor Target Fasting-induced Adipose Factor (FIAF/PGAR/ANGPTL4) Is Present in Blood Plasma as a Truncated Protein That Is Increased by Fenofibrate Treatment

Contact Info

Product

Resources

About

Differential Gene Regulation in Human Versus Rodent Hepatocytes by Peroxisome Proliferator-activated Receptor (PPAR) α

Cited by 171 publications

References 36 publications

Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

Clofibrate Increases Hepatic Triiodothyronine (T3)- and Thyroxine (T4)-Glucuronosyltransferase Activities and Lowers Plasma T3and T4Concentrations in Pigs

The Direct Peroxisome Proliferator-activated Receptor Target Fasting-induced Adipose Factor (FIAF/PGAR/ANGPTL4) Is Present in Blood Plasma as a Truncated Protein That Is Increased by Fenofibrate Treatment

Contact Info

Product

Resources

About

Clofibrate Increases Hepatic Triiodothyronine (T₃)- and Thyroxine (T₄)-Glucuronosyltransferase Activities and Lowers Plasma T₃and T₄Concentrations in Pigs