Differential expression of salt-responsive genes to salinity stress in salt-tolerant and salt-sensitive rice (Oryza sativa L.) at seedling stage

Singh, V. P.; Singh, Ajit; Bhadoria, Jyoti; Giri, Jitender; Singh, Jogendra; Vineeth, T. V.; Sharma, Parbodh Chander

doi:10.1007/s00709-018-1257-6

Cited by 48 publications

(20 citation statements)

References 49 publications

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“…In our study, the excess of Na + prevents K + uptake in both Col-0 and Ws ( Table 1 ). Many studies have reported that maintaining cellular Na + /K + homeostasis is a key factor for salt tolerance ( Mostofa et al, 2015 ; Singh et al, 2018 ). K + is an essential macronutrient involved in not only photoprotection and optimal photosynthetic chain electron transport ( Dukic et al, 2019 ) but also regulation of intracellular and chloroplast osmolarity ( Barragán et al, 2012 ; Tsujii et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

et al. 2021

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Salinity affects plant growth and development as shown with the glycophyte model plant, Arabidopsis thaliana (Arabidopsis). Two Arabidopsis accessions, Wassilewskija (Ws) and Columbia (Col-0), are widely used to generate mutants available from various Arabidopsis seed resources. However, these two ecotypes are known to be salt-sensitive with different degrees of tolerance. In our study, 3-week-old Col-0 and Ws plants were treated with and without 150 mM NaCl for 48, 72, or 96 h, and several physiological and biochemical traits were characterized on shoots to identify any specific traits in their tolerance to salinity. Before salt treatment was carried out, a different phenotype was observed between Col-0 and Ws, whose main inflorescence stem became elongated in contrast to Col-0, which only displayed rosette leaves. Our results showed that Col-0 and Ws were both affected by salt stress with limited growth associated with a reduction in nutrient uptake, a degradation of photosynthetic pigments, an increase in protein degradation, as well as showing changes in carbohydrate metabolism and cell wall composition. These traits were often more pronounced in Col-0 and occurred usually earlier than in Ws. Tandem Mass Tags quantitative proteomics data correlated well with the physiological and biochemical results. The Col-0 response to salt stress was specifically characterized by a greater accumulation of osmoprotectants such as anthocyanin, galactinol, and raffinose; a lower reactive oxygen detoxification capacity; and a transient reduction in galacturonic acid content. Pectin degradation was associated with an overaccumulation of the wall-associated kinase 1, WAK1, which plays a role in cell wall integrity (CWI) upon salt stress exposure. Under control conditions, Ws produced more antioxidant enzymes than Col-0. Fewer specific changes occurred in Ws in response to salt stress apart from a higher number of different fascilin-like arabinogalactan proteins and a greater abundance of expansin-like proteins, which could participate in CWI. Altogether, these data indicate that Col-0 and Ws trigger similar mechanisms to cope with salt stress, and specific changes are more likely related to the developmental stage than to their respective genetic background.

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Section: Discussionmentioning

confidence: 99%

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

et al. 2021

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“…Previous studies have shown the upregulation of stress-responsive marker genes such as NHX1, CAX, CHX11, NHX2, SOS1, SOD, APX7, CAT and TPC1 which are involved in providing stress tolerance in stress-tolerant plants as compared to stress-sensitive plants (Nath et al, 2016;Singh et al, 2018). We compared the expression of these stress-responsive genes in 2 weeks old seedlings.…”

Section: Stress-responsive Genes Regulation Under Oslecrlkmentioning

confidence: 99%

Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis

Passricha

Saifi

Kharb

et al. 2019

Biotech & Bioengineering

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Sensing stress and activating the downstream signaling pathways is the imperative step for stress response. Lectin receptor‐like kinase (LecRLK) is an important family that plays a key role in sensing stress conditions through lectin receptor and activates downstream signaling by kinase domain. We identified the role of OsLecRLK gene for salinity stress tolerance and hypothesized its role in Na+ extrusion from cell. OsLecRLK overexpression and downregulation (through artificial miRNA) transgenic lines were developed and its comparison with wild‐type (WT) plants were performed overexpression transgenic lines showed better performance, whereas downregulation showed poor performance than WT. Lower accumulation of reactive oxygen species (ROS), malondialdehyde and toxic ion, and a higher level of proline, RWC, ROS scavengers in overexpression lines lead us to the above conclusion. Based on the relative expression of stress‐responsive genes, ionic content and interactome protein, working model highlights the role of OsLecRLK in the extrusion of Na+ ion from the cell. This extrusion is facilitated by a higher expression of salt overly sensitive 1 (Na+/K+ channel) in overexpression transgenic line. Altered expression of stress‐responsive genes and changed biochemical and physiological properties of cell suggests an extensive reprogramming of the stress‐responsive metabolic pathways by OsLecRLK under stress condition, which could be responsible for the salt tolerance capability.

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“…Regulation of gene expression via transcription factors is an important mechanism in plants by which they adapt to salinity stress (Jamil et al, 2011). There are a large number of studies on salinity tolerance in wheat (Huang et al, 2012;Yousfi et al, 2016), rice (Kurotani et al, 2015;Singh et al, 2018) and Arabidopsis (Attia et al, 2011;Omidbakhshfard et al, 2012) that have reported many genes as being differentially expressed during salt stress. One of the objectives of our study was to identify target genes regulated by TraesCS2B02G002700.…”

Section: Genes Expressed During Salt Stressmentioning

confidence: 99%

Evolution and identification of DREB transcription factors in the wheat genome: modeling, docking and simulation of DREB proteins associated with salt stress

Hassan

Berk

Aronsson

2021

Journal of Biomolecular Structure and Dynamics

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Soil salinity and the resulting salt stress it imposes on crop plants is a major problem for modern agriculture. Understanding how salt tolerance mechanisms in plants are regulated is therefore important. One regulatory mechanism is the APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factor family, including dehydration responsive element binding (DREB) transcription factors. By binding to DNA, specifically upstream of genes that play roles in salt tolerance pathways, DREB proteins upregulate expression of these genes. DREB in Triticum aestivum (wheat) cluster in subgroups and in this study by scanning the recently extended predicted proteome of wheat for DREB, we increased the number of members of this sub-family. Using the wheat genome, we identified 576 genes coding for the AP2 domain of which 508 were identified to have one AP2 domain, a characteristic of the DREB/ ERF subfamily. We confirmed the existing four subgroups by sequence-based phylogenetic analyses but also identified 32 new DREB subfamily members, not belonging to any known subgroup. Transcription factor profile inference analysis identified two genes, TraesCS2B02G002700 and TraesCS2D02G015200, being homologous to DREB1A of Arabidopsis thaliana. Based on molecular simulation (25 ns) analysis, TraesCS2B02G002700 with a CCGAC motif was observed to interact very stably with DNA. In silico mutational analysis at the 19 th position in the DREB domain of TraesCS2B02G002700-DNA complex indicated this as a stable part for recognizing and forming interaction with DNA. Moreover, six target genes were predicted having an upstream CCGAC motif regulated by TraesCS2B02G002700. Our study provides an overall framework for exploring the transcription factors in plants and identifying e.g. potential salt stress target genes.

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Differential expression of salt-responsive genes to salinity stress in salt-tolerant and salt-sensitive rice (Oryza sativa L.) at seedling stage

Cited by 48 publications

References 49 publications

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis

Evolution and identification of DREB transcription factors in the wheat genome: modeling, docking and simulation of DREB proteins associated with salt stress

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