Evolution and identification of DREB transcription factors in the wheat genome: modeling, docking and simulation of DREB proteins associated with salt stress

Hassan, Sameer; Berk, Katrin; Aronsson, Henrik

doi:10.1080/07391102.2021.1894980

Cited by 11 publications

(6 citation statements)

References 84 publications

(93 reference statements)

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“…This family regulates salt tolerance by binding to upstream the DNA of genes that function specifically in the salt-tolerant pathway. As a result, the DREB protein increased the expression of these salttolerant genes (Hassan et al, 2022). In addition, we discovered that the biological function of MYB under salt stress was tightly connected to the ginger root gene 122051202.…”

Section: Discussionmentioning

confidence: 92%

Physiological and molecular mechanism of ginger (Zingiber officinale Roscoe) seedling response to salt stress

Liu

Cao

et al. 2023

Front. Plant Sci.

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We used ‘Shannong No.1’ experimental material to simulate higher salt concentration in ginger and analyzed the physiological responses of different parts of ginger seedlings under salt stress. The results showed that salt stress led to a significant decrease in fresh and dry weight of ginger, lipid membrane peroxidation, increased sodium ion content and enhanced activity of antioxidant enzymes. Compared with the control, the overall plant dry weight of ginger under salt stress decreased by about 60%, and the MDA content in roots, stems, leaves, and rhizomes increased by 372.27%, 184.88%, 291.5%, and 171.13%, respectively, and the APX content increased by 188.85%, 165.56%, 195.38%, and 40.08%, respectively. After analysis of the physiological indicators, it was found that the roots and leaves of ginger were the most significantly changed parts. We analyzed the transcriptional differences between ginger roots and leaves by RNA-seq and found that they jointly initiated MAPK signaling pathways in response to salt stress. By combining physiological and molecular indicators, we elucidated the response of different tissues and parts of ginger to salt stress during the seedling stage.

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Section: Discussionmentioning

confidence: 92%

Physiological and molecular mechanism of ginger (Zingiber officinale Roscoe) seedling response to salt stress

Liu

Cao

et al. 2023

Front. Plant Sci.

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“…However, a low Na + and a high K + content are preferred and have previously been reported for salt tolerant genotypes [ 32 ], and high K + accumulation reportedly enhances plant growth under salt stress [ 50 ]. In addition, to understand genes directly linked to the transport and accumulation of Na + and K + during salinity stress in different compartments and tissues, the control and regulation of salinity tolerance mechanisms by transcription factor families, such as WRKY and DREB, need to be better understood [ 51 , 52 ]. That will help elucidate salinity tolerance mechanisms as they have critical roles in regulating genes linked to ion transport, antioxidant defense, and osmotic pressure [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Improved Salinity Tolerance-Associated Variables Observed in EMS Mutagenized Wheat Lines

Lethin

Byrt

Berger

et al. 2022

IJMS

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Salinity tolerance-associated phenotypes of 35 EMS mutagenized wheat lines originating from BARI Gom-25 were compared. Vegetative growth was measured using non-destructive image-based phenotyping. Five different NaCl concentrations (0 to 160 mM) were applied to plants 19 days after planting (DAP 19), and plants were imaged daily until DAP 38. Plant growth, water use, leaf Na+, K+ and Cl− content, and thousand kernel weight (TKW) were measured, and six lines were selected for further analysis. In saline conditions, leaf Na+, K+, and Cl− content variation on a dry weight basis within these six lines were ~9.3, 1.4, and 2.4-fold, respectively. Relative to BARI Gom-25, two (OA6, OA62) lines had greater K+ accumulation, three (OA6, OA10, OA62) had 50–75% lower Na+:K+ ratios, and OA62 had ~30% greater water-use index (WUI). OA23 had ~2.2-fold greater leaf Na+ and maintained TKW relative to BARI Gom-25. Two lines (OA25, OA52) had greater TKW than BARI Gom-25 when grown in 120 mM NaCl but similar Na+:K+, WUI, and biomass accumulation. OA6 had relatively high TKW, high leaf K+, and WUI, and low leaf Na+ and Cl−. Phenotypic variation revealed differing associations between the parameters measured in the lines. Future identification of the genetic basis of these differences, and crossing of lines with phenotypes of interest, is expected to enable the assessment of which combinations of parameters deliver the greatest improvement in salinity tolerance.

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“…Similarly, this model comprises of three-stranded antiparallel β-sheet followed by α-helix and relatively unstructured C'-terminal. By changing various amino acids within the AP2 domain, Hassan et al (2021) determined their role in the formation of the secondary structure. They also showed that some of the model structures had two beta strands and one alpha helix.…”

Section: Discussionmentioning

confidence: 99%

“…They found that TaDREB21-B/D showed alternative exon patterns, TaDREB20-D, TaDREB26-A, TaDREB52-B/D, and TaDREB58-A showed an intron-retention pattern, while only TaDREB3-A/B/D showed an exon-skipping pattern which was found on all three chromosomes. Using sequence-based phylogenetic analyses, Hassan et al (2021) identified 32 new DREB subfamily members, not belonging to any known sub-group.…”

Section: Discussionmentioning

confidence: 99%

Research Article Isolation and characterization of DREB gene in ancestral diploid wheat species from a wheat domestication center

Rustamova¹,

Abdullayeva²,

Huseynova³

2022

Genet. Mol. Res.

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The dehydration responsive element (DRE)binding proteins (DREB) play a role in the signaling network that activates many abiotic stress-responsive genes. We isolated and molecularly characterized the DREB gene from ancestral diploid wheat species growing in Azerbaijan and native to this region. This territory is included in a region that is considered the center of origin of cultivated wheat. One-week-old seedlings of Triticum urartu (Au), Aegilops speltoides (B) and Ae. tauschii (D) were used for genomic DNA extraction. Gene-specific primer pairs were applied forisolation of the DREB gene. The amplification products were purified using a gel extraction kit and sequenced. Data analysis was performed using FGENESH, BLAST, INTERPROSCAN, SMART, MAFFT, ExPASy, ProteinPredict, and PSIPRED tools. Ensembl Plants and NCBI were used as integrative resources. Numerous SNPs and nine microindels were detected in the partial target sequence of the DREB gene in Ae. speltoides. Nonsynonymous SNPs were determined in T. urartu (1 transition and 5 transversions), and Ae. tauschii (2 transitions and 2 transversions). Analysis of amino acid sequences encoded by the putative DREB genes revealed a conserved AP2/ERF domain, with two conserved functional amino acids (14th valine and 19th glutamic acid) that play crucial roles in the recognition of the DNA-binding sequence and two tryptophan rings that determine the geometry of the GCC-box ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 21 (3): gmr19035 S.M. Rustamova et al. 2binding domain. Nuclear localization signal and conserved Ser/Thr-rich region were observed in the corresponding amino acid sequences. One αhelix and three β-sheets were detected in the secondary structure of the AP2 domain. The isolated sequences of DREB gene from T. urartu and Ae. tauschii were confirmed and registered in NCBI with Accession Numbers MZ935739 and MZ935740. Identification of the DREB gene in wheat progenitors and its characterization is important for evaluating their genomic material for possible use to enhance the diversity of wheat cultivated under stress conditions.

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Evolution and identification of DREB transcription factors in the wheat genome: modeling, docking and simulation of DREB proteins associated with salt stress

Cited by 11 publications

References 84 publications

Physiological and molecular mechanism of ginger (Zingiber officinale Roscoe) seedling response to salt stress

Physiological and molecular mechanism of ginger (Zingiber officinale Roscoe) seedling response to salt stress

Improved Salinity Tolerance-Associated Variables Observed in EMS Mutagenized Wheat Lines

Research Article Isolation and characterization of DREB gene in ancestral diploid wheat species from a wheat domestication center

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