Differential expression of photosynthesis-related genes and quantification of gas exchange in rice plants under abiotic stress

Amaral, Marcelo Nogueira do; Arge, Luis Willian Pacheco; Benitez, Letícia Carvalho; Danielowski, Rodrigo; Silveira, Solange Ferreira da Silveira; Farias, Daniel da Rosa; Deuner, Sidnei; Oliveira, Antônio Costa de; Braga, Eugênia Jacira Bolacel; Maia, Luciano Carlos da

doi:10.1007/s11738-016-2176-9

Cited by 14 publications

(8 citation statements)

References 69 publications

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“…Os03t0140200-01 (cytochrome p450 86b1-like), ATP synthase cf1 beta subunit (Os10t0355800-00 and Os12t0207500-00), Os08t0561700-01 (superoxide dismutase), Os04t0438300-01 (stress-response protein nst1 isoform × 2), Os09t0474300-01 (chaperone protein htpg family protein), and Os01t021240001 (drought-induced protein rdi), and several chloroplast-related genes were upregulated during Fe toxicity stress. 44 Under Fe toxicity stress, rice genes OsNAS1, OsNAS2, OsNAAT1, OsDMAS1, TOM1, OsYSL15, OsNRAMP1, OsIRT1, OsIRT2, and OsIRO2 were down-regulated and two Fe-storage genes (OsVIT2 and OSFER) were upregulated, which is probably to prevent Fe toxicity. 18,46 Moreover, several genes that are involved in secondary metabolism, e.g.…”

Section: Discussionmentioning

confidence: 97%

“…43 Previous studies report the Fe toxicity stress affects the activity of various genes and enzymes in rice. The activity of genes in the transcriptomic level is needed for homeostasis mechanism, transport, and survival under Fe toxicity stress condition 18,[44][45][46] and the regulation of gene expression will affect the metabolism in rice. Various genes in rice, e.g.…”

Section: Discussionmentioning

confidence: 99%

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Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Turhadi

Hamim

Ghulamahdi

et al. 2019

Plant Signaling & Behavior

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Iron toxicity stress causes physiological and metabolic changes in rice and other plants. To date, there is little information about the metabolite profile of rice under Fe toxicity conditions. In fact, metabolite has a contribution to the physiological condition of plants. Plant metabolomics is a study of low-molecular weight metabolites in plants under certain conditions. The objective of the research was to investigate physiological and metabolic changes in rice under Fe toxicity stress. Two-week-old seedlings of four rice varieties with various Fe toxicity tolerance levels were stressed hydroponically with 400 ppm FeSO 4. 7H 2 O for 10 d. Numerous physiological characters were observed and untargeted metabolomic analysis was carried out using gas chromatography-mass spectrophotometry (GC-MS). The results showed Fe toxicity induced physiological and metabolite variation in rice. By comparing the metabolites synthesized in Fe toxicity-stressed plants with control plants, it showed that elaidic acid, linoleic acid, and linolenic acid could be as metabolite marker candidates for rice response to Fe toxicity stress. When plants exposed to Fe toxicity stress, elaidic acid increased, whereas linoleic-and linolenic acid decreased. The alteration of fatty acid composition in the root and shoot suggests the alteration of metabolites is one of the tolerance strategies of rice to Fe toxicity stress. This finding offers an insight about the tolerance strategies of rice under Fe toxicity stress related to the maintenance process of the cell membranes during this stress. The genes underlying biosynthesis of the fatty acid could be a target of future research on responsible genes for Fe toxicity tolerance in rice.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Turhadi

Hamim

Ghulamahdi

et al. 2019

Plant Signaling & Behavior

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“…Hence, chloroplasts experience significant ROS-mediated damages under stress. Accordingly, the expression of many nuclear genes targeted to chloroplasts is known to be finely tuned under salt stress in flowering plants ( Zörb et al., 2009 ; Fan et al., 2011 ; Wang et al., 2014 ; do Amaral et al., 2016 ). Also, in P. patens photosynthesis in leafy gametophores is known to increase under salt stress to reduce negative effects of salinity ( Wang et al., 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of ppdnmt2 and Identification of Superoxide Dismutase as a Novel Interactor of DNMT2 in the Moss Physcomitrella patens

Singh

Yadav

Kaushik³

et al. 2020

Front. Plant Sci.

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DNMT2 is a DNA/tRNA cytosine methyltransferase that is highly conserved in structure and function in eukaryotes. In plants however, limited information is available on the function of this methyltransferase. We have previously reported that in the moss Physcomitrella patens , DNMT2 plays a crucial role in stress recovery and tRNA Asp transcription/stability under salt stress. To further investigate the role of PpDNMT2 at genome level, in this study we have performed RNA sequencing of ppdnmt2 . Transcriptome analysis reveals a number of genes and pathways to function differentially and suggests a close link between PpDNMT2 function and osmotic and ionic stress tolerance. We propose PpDNMT2 to play a pivotal role in regulating salt tolerance by affecting molecular networks involved in stress perception and signal transduction that underlie maintenance of ion homeostasis in cells. We also examined interactome of PpDNMT2 using affinity purification (AP) coupled to mass spectrometry (AP-MS). Quantitative proteomic analysis reveals several chloroplast proteins involved in light reactions and carbon assimilation and proteins involved in stress response and some not implicated in stress to co-immunoprecipitate with PpDNMT2. Comparison between transcriptome and interactome datasets has revealed novel association between PpDNMT2 activity and the antioxidant enzyme Superoxide dismutase (SOD), protein turnover mediated by the Ubiquitin-proteasome system and epigenetic gene regulation. PpDNMT2 possibly exists in complex with CuZn-SODs in vivo and the two proteins also directly interact in the yeast nucleus as observed by yeast two-hybrid assay. Taken together, the work presented in this study sheds light on diverse roles of PpDNMT2 in maintaining molecular and physiological homeostasis in P. patens . This is a first report describing transcriptome and interactome of DNMT2 in any land plant.

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“…Although small decreases in the plant water potential could cause stomatal closure and a decrease in the intensity of CO 2 assimilation, there are several physiological and molecular mechanisms that differentiate the level of the damage caused by water stress. Diverse studies carried out in different “cultivasr” have shown that there is a differential expression of genes involved in photosynthesis and gas exchange process which are expressed when plants are affected by different abiotic stress such as: water stress, salt, iron, cold and even light quality ( Do Amaral et al, 2016 ; Liu et al, 2016 ; Saha et al, 2016 ; Vineeth et al, 2016 ). These abiotic stress directly affect on WUE through the gene expression linked to Rubisco, photorespiration process and photosystems functioning.…”

Section: Discussionmentioning

confidence: 99%

Effects of Three Irrigation Strategies on Gas Exchange Relationships, Plant Water Status, Yield Components and Water Productivity on Grafted Carménère Grapevines

et al. 2018

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In the Chilean viticultural industry, Carménère is considered an emblematic cultivar that is cultivated mainly in arid and semi-arid zones. For this reason, it is necessary to use precise irrigation scheduling for improving water use efficiency (WUE), water productivity (WP), yield and wine quality. This study evaluated the effects of three deficit irrigation strategies on gas exchange variables, WUE, WP and yield components in a drip-irrigated Carménère vineyard growing under semi-arid climatic conditions during two consecutive seasons (2011/12 and 2012/13). The irrigation strategies were applied in completely randomized design from fruit set (S) to harvest (H). The first irrigation strategy (T1) involved continuous irrigation at 100% of actual evapotranspiration (ETa) from S to the veraison (V) period and at 80% of ETa from V to H. The second irrigation strategy (T2) involved irrigation at 50% of ETa from S to H and the third one (T3) involved no-irrigation from S to V and at 30% of ETa from V to H. The results indicated that there was a significant non-linear correlation between net CO2 assimilation (AN) and stomatal conductance (gs), which resulted in three zones of water stress (zone I = gs > 0.30 mol H2O m-2s-1; zone II = between 0.06 and 0.30 mol H2O m-2s-1; and zone III = gs < 0.06 mol H2O m-2s-1). The use of less water by T2 and T3 had a significant effect on yield components, with a reduction in the weight and diameter of grapes. A significant increase in WP (7.3 kg m-3) occurred in T3, which resulted in values of WUE that were significantly higher than those from T1 and T2. Also, a significant non-linear relationship between the integral water stress (SIΨ) and WP (R2 = 0.74) was established. The results show that grafted Carménère vines were tolerant to water stress although differences between cultivars/genotypes still need to be evaluated.

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Differential expression of photosynthesis-related genes and quantification of gas exchange in rice plants under abiotic stress

Cited by 14 publications

References 69 publications

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Transcriptome Analysis of ppdnmt2 and Identification of Superoxide Dismutase as a Novel Interactor of DNMT2 in the Moss Physcomitrella patens

Effects of Three Irrigation Strategies on Gas Exchange Relationships, Plant Water Status, Yield Components and Water Productivity on Grafted Carménère Grapevines

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