Most hypotheses explaining the general gradient of higher diversity toward the equator are implicit or explicit about greater species packing in the tropics. However, global patterns of diversity within guilds, including trophic guilds (i.e., groups of organisms that use similar food resources), are poorly known. We explored global diversity patterns of a key trophic guild in stream ecosystems, the detritivore shredders. This was motivated by the fundamental ecological role of shredders as decomposers of leaf litter and by some records pointing to low shredder diversity and abundance in the tropics, which contrasts with diversity patterns of most major taxa for which broad-scale latitudinal patterns haven been examined. Given this evidence, we hypothesized that shredders are more abundant and diverse in temperate than in tropical streams, and that this pattern is related to the higher temperatures and lower availability of high-quality leaf litter in the tropics. Our comprehensive global survey (129 stream sites from 14 regions on six continents) corroborated the expected latitudinal pattern and showed that shredder distribution (abundance, diversity and assemblage composition) was explained by a combination of factors, including water temperature (some taxa were restricted to cool waters) and biogeography (some taxa were more diverse in particular biogeographic realms). In contrast to our hypothesis, shredder diversity was unrelated to leaf toughness, but it was inversely related to litter diversity. Our findings markedly contrast with global trends of diversity for most taxa, and with the general rule of higher consumer diversity at higher levels of resource diversity. Moreover, they highlight the emerging role of temperature in understanding global patterns of diversity, which is of great relevance in the face of projected global warming.

AimWe tested the hypothesis that shredder detritivores, a key trophic guild in stream ecosystems, are more diverse at higher latitudes, which has important ecological implications in the face of potential biodiversity losses that are expected as a result of climate change. We also explored the dependence of local shredder diversity on the regional species pool across latitudes, and examined the influence of environmental factors on shredder diversity.Location World-wide (156 sites from 17 regions located in all inhabited continents at latitudes ranging from 67°N to 41°S). MethodsWe used linear regression to examine the latitudinal variation in shredder diversity at different spatial scales: alpha (a), gamma (g) and beta (b) diversity. We also explored the effect of g-diversity on a-diversity across latitudes with regression analysis, and the possible influence of local environmental factors on shredder diversity with simple correlations.Results Alpha diversity increased with latitude, while g-and b-diversity showed no clear latitudinal pattern. Temperate sites showed a linear relationship between g-and a-diversity; in contrast, tropical sites showed evidence of local species saturation, which may explain why the latitudinal gradient in a-diversity is not accompanied by a gradient in g-diversity. Alpha diversity was related to several local habitat characteristics, but g-and b-diversity were not related to any of the environmental factors measured. Main conclusionsOur results indicate that global patterns of shredder diversity are complex and depend on spatial scale. However, we can draw several conclusions that have important ecological implications. Alpha diversity is limited at tropical sites by local factors, implying a higher risk of loss of key species or the whole shredder guild (the latter implying the loss of trophic diversity). Even if regional species pools are not particularly species poor in the tropics, colonization from adjacent sites may be limited. Moreover, many shredder species belong to cool-adapted taxa that may be close to their thermal maxima in the tropics, which makes them more vulnerable to climate warming. Our results suggest that tropical streams require specific scientific attention and conservation efforts to prevent loss of shredder biodiversity and serious alteration of ecosystem processes.

Grape pomace was extracted with pressurized hot water at laboratory scale before and after fermentation to explore the effects of fermentation and extraction temperature (50-200 °C) and time (5 and 30 min) on total extracted antioxidant levels and activity and to determine the content and recovery efficiency of main grape polyphenols, anthocyanins, and tannins. Fermented pomace yielded more total antioxidants (TAs), antioxidant activity, and tannins, than unfermented pomace but fewer anthocyanins. Elevating the extraction temperature increased TA extraction and antioxidant activity. Maximum anthocyanin extraction yields were achieved at 100 °C and at 150 °C for tannins and tannin-anthocyanin adducts. Using higher temperatures and longer extraction times resulted in a sharp decrease of polyphenol extraction yield. Relevant proanthocyanidin amounts were extracted only at 50 and 100 °C. Finally, TA recovery and activity were not directly related to the main polyphenol content when performing pressurized hot water grape pomace extraction.

A field experiment was carried out to implement a remote sensing energy balance (RSEB) algorithm for estimating the incoming solar radiation (Rsi), net radiation (Rn), sensible heat flux (H), soil heat flux (G) and latent heat flux (LE) over a drip-irrigated olive (cv. Arbequina) orchard located in the Pencahue Valley, Maule Region, Chile (35˝25 1 S; 71˝44 1 W; 90 m above sea level). For this study, a helicopter-based unmanned aerial vehicle (UAV) was equipped with multispectral and infrared thermal cameras to obtain simultaneously the normalized difference vegetation index (NDVI) and surface temperature (T surface ) at very high resolution (6 cmˆ6 cm). Meteorological variables and surface energy balance components were measured at the time of the UAV overpass (near solar noon). The performance of the RSEB algorithm was evaluated using measurements of H and LE obtained from an eddy correlation system. In addition, estimated values of Rsi and Rn were compared with ground-truth measurements from a four-way net radiometer while those of G were compared with soil heat flux based on flux plates. Results indicated that RSEB algorithm estimated LE and H with errors of 7% and 5%, respectively. Values of the root mean squared error (RMSE) and mean absolute error (MAE) for LE were 50 and 43 W m´2 while those for H were 56 and 46 W m´2, respectively. Finally, the RSEB algorithm computed Rsi, Rn and G with error less than 5% and with values of RMSE and MAE less than 38 W m´2. Results demonstrated that multispectral and thermal cameras placed on an UAV could provide an excellent tool to evaluate the intra-orchard spatial variability of Rn, G, H, LE, NDVI and T surface over the tree canopy and soil surface between rows.

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-Shredders play a major ecological role in temperate streams, but their numerical importance is highly variable within the tropics. Detailed studies on the diets of tropical stream invertebrates are advisable to be able to better describe and understand this variation. Here, we examined the diets of invertebrates collected from the leaf litter of three tropical streams in Colombia, using gut content analysis. Fine and coarse particulate organic matter were the main food resources for invertebrates, which could be divided into four main dietary groups: predators, shredders, specialist collectors and generalist collectors. While the specialist collectors were the most numerically abundant group (54%), shredder biomass accounted for 63% of total invertebrate biomass, suggesting that shredders play a significant ecological role in the study streams. We describe the diets of 12 out of 47 taxa that were previously unknown, which indicates that knowledge about the feeding ecology of tropical stream invertebrates is still incipient.

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Aerial and terrestrial thermography has become a practical tool to determine water stress conditions in vineyards. However, for proper use of this technique it is necessary to consider vine architecture (canopy zone analysis) and image thresholding approaches (determination of the upper and lower baseline temperature values). During the 2014-2015 growing season, an experimental study under different water conditions (slight, mild, moderate, and severe water stress) was carried out in a commercial vineyard (Vitis vinifera L., cv. Carménè). In this study thermal images were obtained from different canopy zones by using both aerial (>60 m height) and ground-based (sunlit, shadow and nadir views) thermography. Using customized code that was written specifically for this research, three different thresholding approaches were applied to each image: (i) the standard deviation technique (SDT); (ii) the energy balance technique (EBT); and (iii) the field reference temperature technique (FRT). Results obtained from three different approaches showed that the EBT had the best performance. The EBT was able to discriminate over 95% of the leaf material, while SDT and FRT were able to detect around 70% and 40% of the leaf material, respectively. In the case of canopy zone analysis, ground-based nadir images presented the best correlations with stomatal conductance (g s ) and stem water potential (Ψ stem ), reaching determination coefficients (r 2 ) of 0.73 and 0.82, respectively. The best relationships between thermal indices and plant-based variables were registered during the period of maximum atmospheric demand (near veraison) with significant correlations for all methods.