Differential expression of microRNAs let‐7a, miR‐125b, miR‐100, and miR‐21 and interaction with NF‐kB pathway genes in periodontitis pathogenesis

Sriram, Damal Kandadai; Koshy, Teena; Lavu, Vamsi; Rao, Suresh Ranga; Selvaraj, Raja; Hariharan, Samyuktha; Venkatesan, Vettriselvi

doi:10.1002/jcp.26391

Cited by 48 publications

(42 citation statements)

References 25 publications

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“…MiR-21, being one of the most multi-functional miRs, is usually investigated in tumor related diseases (Selcuklu et al, 2009 ). However, increasingly intensive studies involving miR-21 have led researchers to conclude that miR-21 also plays an indispensable role in inflammation (Sheedy, 2015 ; Li et al, 2017 ; Venugopal et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

A Novel Murine Chronic Obstructive Pulmonary Disease Model and the Pathogenic Role of MicroRNA-21

Sun

et al. 2018

Front. Physiol.

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Chronic obstructive pulmonary disease (COPD) is a multi-pathogenesis chronic lung disease. The mechanisms underlying COPD have not been adequately illustrated. Many reseachers argue that microRNAs (miRs) could play a crucial role in COPD. The classic animal model of COPD is both time consuming and costly. This study proposes a novel mice COPD model and explores the role of miR-21 in COPD. A total of 50 wide-type (WT) C57BL/6 mice were separated into five euqlly-sized groups—(1) control group (CG), (2) the novel combined method group (NCM, cigarette smoke (CS) exposure for 28 days combined with cigarette smoke extract (CSE) intraperitoneal injection), (3) the short-term CS exposure group (SCSE, CS exposure for 28 days), (4) the CSE intraperitoneal injection group (CSEII, 28 days CSE intraperitoneal injection), and (5) the long-term CS exposure group (LCSE, CS exposure).The body weight gain of mice were recorded and lung function tested once the modeling was done. The pathological changes and the inflammation level by hematoxylin eosin (H&E) staining and immunohistochemical staining (IHS) on the lung tissue sections were also evaluated. The level of miR-21 in the mice lungs of the mice across all groups was detected by RT-qPCR and the effects of miR-21 knock-down in modeled mice were observed. The mice in LCSE and NCM exhibited the most severe inflammation levels and pathological and pathophysiological changes; while the changes for the mice in SCSE and CSEII were less, they remained more severe than the mice in the CG. The level of miR-21 was found to be negatively correlated with lung functions. Moreover, knocking miR-21 down from the modeled mice, ameliorated all those tested COPD-related changes. Our novel modeling method detected virtually the same changes as those detected in the classic method in WT mice, but in less time and cost. Further, it was determined that the level of miR-21 in the lungs could be an indicator of COPD severity and blocking functions of miR-21 could be a potential treatment for early stage COPD.

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Section: Introductionmentioning

confidence: 99%

A Novel Murine Chronic Obstructive Pulmonary Disease Model and the Pathogenic Role of MicroRNA-21

Sun

et al. 2018

Front. Physiol.

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“…Another study reported the upregulation of let‐7a and miR‐21 as well as the reduction in miR‐125b and miR‐100 in inflamed gingiva from chronic periodontitis patients. All these four miRNAs targeted nuclear factor kappa B ( NF κ B ), which was specific to inflammation . These two studies investigated the correlation between canonical miRNA molecules and chronic periodontitis.…”

Section: Introductionmentioning

confidence: 99%

“…All these four miRNAs targeted nuclear factor kappa B (NFκB), which was specific to inflammation. 9 These two studies investigated the correlation between canonical miRNA molecules and chronic periodontitis. Nevertheless, more distinctive miRNAs featured in periodontal inflammation remained to be discovered.…”

Section: Introductionmentioning

confidence: 99%

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Wang

Chen

et al. 2018

J of Periodontal Research

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Background and Objective This study aimed to discover the distinctive MicroRNAs (miRNA) functioning in the pathogenesis of periodontal inflammation, which might be potential therapy targets of chronic periodontitis. Material and Methods miRNA profiles of human inflamed gingival tissue from three previous microarrays were re‐analysed. Gingival tissues were collected for the validation of overlapping miRNAs, and a network was constructed to show regulatory connection between overlapping miRNAs and periodontitis‐associated target genes. Potential miRNAs were screened based on their expression levels and predicted target genes. Correlation analysis and binding site prediction were conducted to reveal the relationship between the potential miRNAs and their target genes. Results miR‐144‐5p, found to be upregulated in all three studies, showed the greatest upregulation (P < 0.0001). Another 16 miRNAs (10 upregulated and six downregulated) overlapped between any two of the three studies. All overlapping miRNAs had expected expression levels except for miR‐203 during validation. Ten miRNAs (six upregulated and four downregulated) were found to have periodontal inflammation‐associated targets. Cyclooxygenase 2 (COX2) and interleukin‐17F (IL17F), predicted target genes of upregulated miR‐144‐5p, showed significant decreases and were negatively correlated with miR‐144‐5p in the periodontitis group (r = −0.742 for COX2, r = −0.615 for IL17F). Conclusion This re‐analysis of miRNA signatures has implied the potential regulatory mechanism of miR‐144‐5p and its potential for exploring alternative therapeutic approaches, especially those that use miRNA delivery systems to treat chronic periodontitis. Nevertheless, further study based on larger sample size and homogenous cells is needed to reveal the exact roles of miRNAs in chronic periodontitis.

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“…The intense inflammatory condition that characterizes periodontitis includes the involvement of several pathways; in particular, the correlation between NF-κB and periodontitis is widely known, as demonstrated by several clinical studies [ 38 , 39 ]. Lut 30 mg/kg decreased the levels of NF-κB and increased the expression of the cytosolic protein IκB-α, as shown from Western blot analysis performed.…”

Section: Discussionmentioning

confidence: 99%

Treatment with Luteolin Improves Lipopolysaccharide-Induced Periodontal Diseases in Rats

et al. 2020

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Periodontitis is a dental disease that produces the progressive destruction of the bone surrounding the tooth. Especially, lipopolysaccharide (LPS) is involved in the deterioration of the alveolar bone, inducing the release of pro-inflammatory mediators, which cause periodontal tissue inflammation. Luteolin (Lut), a molecule of natural origin present in a large variety of fruits and vegetables, possess beneficial properties for human health. On this basis, we investigated the anti-inflammatory properties of Lut in a model of periodontitis induced by LPS in rats. Animal model predicted a single intragingival injection of LPS (10 μg/μL) derived from Salmonella typhimurium. Lut administration, was performed daily at different doses (10, 30, and 100 mg/kg, orally), starting from 1 h after the injection of LPS. After 14 days, the animals were sacrificed, and their gums were processed for biochemical analysis and histological examinations. Results showed that Lut (30 and 100 mg/kg) was equally able to reduce alveolar bone loss, tissue damage, and neutrophilic infiltration. Moreover, Lut treatment reduced the concentration of collagen fibers, mast cells degranulation, and NF-κB activation, as well as the presence of pro-inflammatory enzymes and cytokines. Therefore, Lut implementation could represent valid support in the pharmacological strategy for periodontitis, thus improving the well-being of the oral cavity.

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Differential expression of microRNAs let‐7a, miR‐125b, miR‐100, and miR‐21 and interaction with NF‐kB pathway genes in periodontitis pathogenesis

Cited by 48 publications

References 25 publications

A Novel Murine Chronic Obstructive Pulmonary Disease Model and the Pathogenic Role of MicroRNA-21

A Novel Murine Chronic Obstructive Pulmonary Disease Model and the Pathogenic Role of MicroRNA-21

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Treatment with Luteolin Improves Lipopolysaccharide-Induced Periodontal Diseases in Rats

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