Differential expression of angiogenesis-related miRNAs and VEGFA in cirrhosis and hepatocellular carcinoma

Oliveira, André Rodrigueiro Clavisio Pereira; Castanhole-Nunes, Márcia Maria Urbanin; Biselli-Chicote, Patrícia Matos; Pavarino, Érika Cristina; Silva, Rita de C. M. A. da; Silva, Renato F. da; Goloni-Bertollo, Eny Maria

doi:10.5114/aoms.2020.97967

Cited by 32 publications

(21 citation statements)

References 74 publications

(83 reference statements)

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“…We detected and validated downregulation of miR-1255b-5p in TAs of severe BPD infants. This miRNA was previously noted to regulate vascular endothelial growth factor A (VEGFA) expression in liver cirrhosis and hepatocellular carcinoma [63]. Abnormal VEGF and disrupted angiogenesis are described in lung tissues of infants that died of severe BPD [64].…”

Section: Discussionmentioning

confidence: 94%

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

Siddaiah¹,

Oji‐Mmuo²,

Montes³

et al. 2021

Preprint

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Bronchopulmonary dysplasia (BPD) is a form of chronic lung disease that develops in neonates as a consequence of preterm birth and arrested fetal lung development. The incidence of BPD remains on the rise, as a result of increasing survival of extremely preterm infants. Severe BPD contributes to significant health care costs and is associated with prolonged hospitalizations, respiratory infections, and neurodevelopmental deficits. In this study, we aimed to detect novel biomarkers of severe BPD. We collected tracheal aspirates (TA) from preterm babies with mild/moderate (n = 8) and severe (n = 17) BPD, and we profiled the expression of 1048 miRNAs using a PCR array. Associations with biological pathways were determined with the Ingenuity Pathway Analysis (IPA) software. We found 31 miRNAs differentially expressed between the two disease groups (2-fold change, FDR &lt; 0.05). Of these, 4 miRNAs displayed significantly higher expression levels, and 27 miRNAs had significantly lower expression levels in the severe BPD vs. the mild/moderate BPD group. IPA identified cell signaling and inflammation pathways associated with miRNA signatures. We conclude that TAs of extreme premature infants contain miRNA signatures associated with severe BPD. These signatures may serve as biomarkers of disease severity in infants with BPD.

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Section: Discussionmentioning

confidence: 94%

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

Siddaiah¹,

Oji‐Mmuo²,

Montes³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…We detected and validated the downregulation of miR-1255b-5p in TAs of severe BPD infants. This miRNA was previously noted to regulate vascular endothelial growth factor A (VEGFA) expression in liver cirrhosis and hepatocellular carcinoma [ 72 ]. Abnormal VEGF and disrupted angiogenesis are described in lung tissues of infants that died of severe BPD [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Signatures Associated with Bronchopulmonary Dysplasia Severity in Tracheal Aspirates of Preterm Infants

et al. 2021

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Bronchopulmonary dysplasia (BPD) is a form of chronic lung disease that develops in neonates as a consequence of preterm birth, arrested fetal lung development, and inflammation. The incidence of BPD remains on the rise as a result of increasing survival of extremely preterm infants. Severe BPD contributes to significant health care costs and is associated with prolonged hospitalizations, respiratory infections, and neurodevelopmental deficits. In this study, we aimed to detect novel biomarkers of BPD severity. We collected tracheal aspirates (TAs) from preterm babies with mild/moderate (n = 8) and severe (n = 17) BPD, and we profiled the expression of 1048 miRNAs using a PCR array. Associations with biological pathways were determined with the Ingenuity Pathway Analysis (IPA) software. We found 31 miRNAs differentially expressed between the two disease groups (2-fold change, false discovery rate (FDR) < 0.05). Of these, 4 miRNAs displayed significantly higher expression levels, and 27 miRNAs had significantly lower expression levels in the severe BPD group when compared to the mild/moderate BPD group. IPA identified cell signaling and inflammation pathways associated with miRNA signatures. We conclude that TAs of extremely premature infants contain miRNA signatures associated with severe BPD. These may serve as potential biomarkers of disease severity in infants with BPD.

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“…Vascular endothelial growth factor (VEGF) is an essential angiogenic growth factor in physiological and pathological states. High expression of VEGFA is detected in a large number of solid tumors, including HCC [32,33] . It is involved in the regulation of metastasis of many solid tumors and their neovasculature [14,34] .…”

Section: Discussionmentioning

confidence: 99%

Spatial Maps of Hepatocellular Carcinoma Transcriptomes Highlight an Unexplored Landscape of Heterogeneity and a Novel Gene Signature for Survival

Zhao

Zhang

Cheng

et al. 2021

Preprint

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Background: Hepatocellular carcinoma (HCC) are often present with satellite nodules, rendering current curative treatments ineffective in many patients. The heterogeneity of HCC is a major challenge in personalized medicine. The emergence of spatial transcriptomics (ST) provides a powerful strategy for delineating the complex molecular landscapes of tumors. Methods: In this study, we investigated tissue-wide gene expression heterogeneity in tumor and adjacent nonneoplastic tissues using ST technology. We analyzed the transcriptomes of nearly 10820 tissue regions and identified main gene expression clusters and their specific marker genes (differentially expressed genes, DEGs) in patients. The DEGs were analyzed from two perspectives. First of all, we identified two distinct gene profiles associated with satellite nodules and conducted a more comprehensive analysis for both gene profiles. Their clinical relevance for human HCC was validated with KM Plotter. Secondly, we screened DEGs with TCGA database to divide the HCC cohort into high- and low-risk groups according to Cox analysis. HCC patients from the ICGC cohort were used for validation. Kaplan Meier analysis was used to compare the overall survival (OS) between high- and low-risk groups. Univariate and multivariate Cox analyses were applied to determine the independent predictors for OS. Results: Novel markers for the prediction of satellite nodules and a tumor clusters specific marker genes signature model(6 genes) for HCC prognosis was constructed, respectively. Conclusion: The establishment of marker gene proﬁles may be an important step towards an unbiased view of HCC and the 6-genes signature can be used for prognostic prediction in HCC. This analysis will help us to clarify one of the possible soucres of HCC heterogeneity, uncover pathogenic mechanisms and novel anti-tumor drug targets.

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Differential expression of angiogenesis-related miRNAs and VEGFA in cirrhosis and hepatocellular carcinoma

Cited by 32 publications

References 74 publications

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

MicroRNA Signatures Associated with Bronchopulmonary Dysplasia Severity in Tracheal Aspirates of Preterm Infants

Spatial Maps of Hepatocellular Carcinoma Transcriptomes Highlight an Unexplored Landscape of Heterogeneity and a Novel Gene Signature for Survival

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