Differential effects of sucrose and fructose on dietary obesity in four mouse strains

Glendinning, John I.; Breinager, Lindsey; Kyrillou, Emily; Lacuna, Kristine; Rocha, Rotsen; Sclafani, Anthony

doi:10.1016/j.physbeh.2010.06.003

Cited by 64 publications

(51 citation statements)

References 86 publications

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“…Thus, the concentration of sucrose was increased to 34% for 60 more days, totalizing 4 months of treatment. The 34% sucrose concentration has been tested in mice by Glendinning et al (2010). Water intake was measured weekly to verify the water intake and the water with sucrose intake.…”

Section: Ethical Aspects Animals and Treatmentsmentioning

confidence: 99%

High consumption of sucrose induces DNA damage in male Wistar rats

Franke

Molz

Mai

et al. 2017

An. Acad. Bras. Ciênc.

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The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p<0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p<0.05) in hippocampal cells (~60%) but not in peripheral blood leukocytes (p>0.05). Additionally, it was observed a significative increase (p<0.05) in the markers of chromosomal breaks/losses in bone marrow, as indicated by the micronucleus test. This is the first study that evaluated DNA damage induced by high sucrose concentration in the hippocampus and bone marrow of rats. Sucrose-induced DNA damage was observed in both tissues. However, the mechanism of sucrose toxicity on DNA remains unknown.

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Section: Ethical Aspects Animals and Treatmentsmentioning

confidence: 99%

High consumption of sucrose induces DNA damage in male Wistar rats

Franke

Molz

Mai

et al. 2017

An. Acad. Bras. Ciênc.

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“…In order to induce prediabetes in the animals we used an overload of invert sugar (Pantec ® , São Paulo, São Paulo, Brazil) dissolved in water at a concentration of 32% 17 .…”

Section: Test Substances: Induction Of Prediabetes and Vitamin C Suppmentioning

confidence: 99%

Vitamin C decreases the obesogenic and hyperglycemic effect of invert sugar in prediabetic rats

Molz¹,

Rael

Fischer

et al. 2017

Rev. Nutr.

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Objective: To evaluate whether vitamin C can help to prevent obesity and hyperglycemia in Wistar rats treated with excess invert sugar to induce prediabetes. Methods: One hundred-day-old Male Wistar rats with a mean weight of 336.58±23.43g were randomly assigned to the following groups: (1) control, receiving water (C); (2) invert sugar control, receiving a 32% watery solution of invert sugar; (3) vitamin C control, receiving a watery solution of vitamin C (60mg/L), and (4) vitamin C plus invert sugar, receiving a watery solution of vitamin C and invert sugar. All animals had access to chow and water ad libitum and were treated for 17 weeks. Prediabetes was assessed according to two criteria: obesity (based on body mass indexand peritoneal fat content) and impaired glucose tolerance (assessed by the intraperitoneal glucose tolerance test and expressed as area under the curve) . Results: Group invert sugar control gained significantly more weight (p=0.035) and visceral fat (p<0.001) than groups vitamin C control and vitamin C plus invert sugar. Consequently, groups vitamin C control and vitamin C plus invert sugar had gained as little body mass index as group C by the end of the experiment. Vitamin C decreased the fasting glycemia of both groups supplemented with vitamin C and normalized the glucose tolerance of group vitamin C plus invert sugar, whose area under the curve matched that of group C. Conclusion: Vitamin C has anti-obesogenic and glycemia-lowering effects in Wistar rats, which might be promising to prediabetics. Future studies are needed to understand the anti-obesogenic and anti-hyperglycemic mechanisms of vitamin C in prediabetes.

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“…We selected this inbred strain based on an earlier observation that FVB mice consumed substantially more 10% fructose than did B6 mice when given ad libitum access to sugar solution, water, and food (15). The FVB mice also consumed more 10% sucrose than did B6 mice, but this difference was less pronounced than that observed with fructose.…”

mentioning

confidence: 99%

Fructose- and glucose-conditioned preferences in FVB mice: strain differences in post-oral sugar appetition

Sclafani

Zukerman

Ackroff

2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Recent studies indicate that, unlike glucose, fructose has little or no post-oral preference conditioning actions in C57BL/6J (B6) mice. The present study determined whether this is also the case for FVB mice, which overconsume fructose relative to B6 mice. In experiment 1, FVB mice strongly preferred a noncaloric 0.1% sucralose ϩ 0.1% saccharin (SϩS) solution to 8% fructose in a 2-day choice test but switched their preference to fructose after separate experience with the two sweeteners. Other FVB mice displayed a stronger preference for 8% glucose over SϩS. In a second experiment, ad libitum-fed FVB mice trained 24 h/day acquired a significant preference for a flavor (CSϩ) paired with intragastric (IG) selfinfusions of 16% fructose over a different flavor (CSϪ) paired with IG water infusions. IG fructose infusions also conditioned flavor preferences in food-restricted FVB mice trained 1 h/day. IG infusions of 16% glucose conditioned stronger preferences in FVB mice trained 24-or 1 h/day. Thus, fructose has post-oral flavor conditioning effects in FVB mice, but these effects are less pronounced than those produced by glucose. Further studies of the differential post-oral conditioning effects of fructose and glucose in B6 and FVB mice should enhance our understanding of the physiological processes involved in sugar reward.post-oral flavor conditioning; sucralose; saccharin; C57BL/6J mice IT IS WELL ESTABLISHED THAT inbred mouse strains differ in their taste responses to caloric and noncaloric sweeteners. This is due, in part, to allelic variations in the Tas1r3 gene that encodes the T1r3 sweet taste receptor protein (6,25). Mouse strains with the Sac B variant of the Tas1r3 gene are more sensitive to sugars (e.g., sucrose) and noncaloric sweeteners (e.g., saccharin) than are strains with the Sac D variant. In addition, sugar intake and preference are stimulated by the post-oral actions of sugars, a process referred to as appetition to distinguish it from the satiation process that inhibits sugar intake (27). Appetition is demonstrated by the intake and preference-stimulating effects of intragastric (IG) sugar infusions in mice and rats (3,21,27,30). Conceivably, strain differences in post-oral sugar appetition may contribute to variations in sugar intake in mice, but this has yet to be documented. One study reported mouse strain differences in sugar-conditioned flavor preferences, but because the sugars were orally consumed, the conditioning effects may have been mediated by the taste and/or post-oral actions of the sugars (24). In another study, we compared IG sucrose conditioning in The present study sought evidence for strain differences in the post-oral conditioning response to glucose and fructose, the constituent monosaccharide sugars of sucrose. B6 mice acquire strong preferences for flavors paired with IG glucose infusions but fail to prefer flavors paired with isocaloric fructose infusions (29, 43). Related findings were obtained with B6 mice given oral choice tests with 8% glucose or 8% fructose vs. a ...

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Differential effects of sucrose and fructose on dietary obesity in four mouse strains

Cited by 64 publications

References 86 publications

High consumption of sucrose induces DNA damage in male Wistar rats

High consumption of sucrose induces DNA damage in male Wistar rats

Vitamin C decreases the obesogenic and hyperglycemic effect of invert sugar in prediabetic rats

Fructose- and glucose-conditioned preferences in FVB mice: strain differences in post-oral sugar appetition

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