High consumption of sucrose induces DNA damage in male Wistar rats

Franke, Silvia Isabel Rech; Molz, Patrí­cia; Mai, Camila; Ellwanger, Joel Henrique; Zenkner, Fernanda Fleig; Horta, Jorge André; Prá, Daniel

doi:10.1590/0001-3765201720160659

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…The obtained results evidenced an increase in LC3-II levels following the administration of trehalose and raffinose as compared with all the other treatments. Both trehalose and raffinose did not show any cytotoxic effect, as demonstrated by others [42,43]. Among these, trehalose induced an increase of expression of other autophagy markers, i.e., Beclin1 and ATG5, as already documented [40].…”

Section: Discussionsupporting

confidence: 77%

Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model

Manai

Azzalin

Morandi

et al. 2019

Cells

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Celiac disease (CD) is a chronic systemic autoimmune disorder that is triggered by the ingestion of gliadin peptides, the alcohol-soluble fraction of wheat gluten. These peptides, which play a key role in the immune response that underlies CD, spontaneously form aggregates and exert a direct toxic action on cells due to the increase in the reactive oxygen species (ROS) levels. Furthermore, peptic-tryptic digested gliadin peptides (PT-gliadin) lead to an impairment in the autophagy pathway in an in vitro model based on Caco-2 cells. Considering these premises, in this study we have analyzed different mTOR-independent inducers, reporting that the disaccharide trehalose, a mTOR-independent autophagy activator, rescued the autophagy flux in Caco-2 cells treated with digested gliadin, as well as improved cell viability. Moreover, trehalose administration to Caco-2 cells in presence of digested gliadin reduced the intracellular levels of these toxic peptides. Altogether, these results showed the beneficial effects of trehalose in a CD in vitro model as well as underlining autophagy as a molecular pathway whose modulation might be promising in counteracting PT-gliadin cytotoxicity.

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Section: Discussionsupporting

confidence: 77%

Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model

Manai

Azzalin

Morandi

et al. 2019

Cells

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“…In brain cells, there is an association between high consumption of sucrose (a disaccharide formed by glucose and fructose) and increased levels of DNA damage, suggesting that DNA glycation reactions by fructose may be responsible for the damage found in brain cells in the present work. 52 Corroborating this, Yamazaki et al 53 found that excessive maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification in brain-derived neurotrophic factor (BDNF) gene expression. 53 The hippocampus was the most affected area with reduced antioxidant activity and sulfhydryl content, leading to increased oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

Metabolic programming in offspring of mice fed fructose during pregnancy and lactation

Magenis¹,

Damiani²,

Silveira

et al. 2021

J Dev Orig Health Dis

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Fructose (C6H12O6), also known as levulose, is a hexose. Chronic consumption of fructose may be associated with increased intrahepatic fat concentration and the development of insulin resistance as well as an increase in the prevalence of nonalcoholic fatty liver disease and hyperlipidemia during pregnancy. Despite the existence of many studies regarding the consumption of fructose in pregnancy, its effects on fetuses have not yet been fully elucidated. Therefore, the objective of this study was to evaluate the genetic and biochemical effects in offspring (male and female) of female mice treated with fructose during pregnancy and lactation. Pairs of 60-day-old Swiss mice were used and divided into three groups; negative control and fructose, 10%/l and 20%/l doses of fructose groups. After offspring birth, the animals were divided into six groups: P1 and P2 (males and females), water; P3 and P4 (males and females) fructose 10%/l; and P5 and P6 (males and females) fructose 20%/l. At 30 days of age, the animals were euthanized for genetic and biochemical assessments. Female and male offspring from both dosage groups demonstrated genotoxicity (evaluated through comet assay) and oxidative stress (evaluated through nitrite concentration, sulfhydril content and superoxide dismutase activity) in peripheral and brain tissues. In addition, they showed nutritional and metabolic changes due to the increase in food consumption, hyperglycemia, hyperlipidemia, and metabolic syndrome. Therefore, it is suggested that high consumption of fructose by pregnant female is harmful to their offspring. Thus, it is important to carry out further studies and make pregnant women aware of excessive fructose consumption during this period.

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“…Early efforts to understand the effects of carbohydrate metabolism and DNA damage were made using Saccharomyces cerevisiae as a model and a systems biology approach (Barea and Bonatto 2008), showing that reactive carbonyl species (RCS) provided by sugar metabolism could be linked to DNA damage. The manuscript of Franke et al (2017) also addresses this question, and shows that the consumption of a high sucrose diet in rat model promotes DNA damage in hippocampus hence it contributes to the development of diabetes-associated neuropathologies. …”

mentioning

confidence: 99%

Landslide hazard mapping, DNA damage induced by sucrose and the biotechnology potential of sponge-associated bacteria communities

Bonatto

2017

An. Acad. Bras. Ciênc.

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Since its foundation in 1929, the major concern of Annals of The Brazilian Academy of Sciences (AABC) has been to promote scientific investigations in different research fields that could improve the welfare of our populations. In fact, the current number of AABC clearly displays this long-standing editorial tradition.The growth of population in a worldwide scale and the social vulnerability of different human communities subjected to war, economy collapse, ethnical persecution and poverty impose a strong pressure to occupy multi-hazard environments, like flood plains and hill slopes subject to landslides (Sullivan-Wiley and Gianotti 2017). As human communities occupy unstable hill slope areas, activities like deforestation or irregular building sites increase the probability of landslide occurrence (Dai et al. 2002). Thus, it becomes imperative to assess the regional factors that could trigger a landslide event. The manuscript of Cerri et al.(2017) invokes a new geologic strategy to evaluate potential landslide regions in Serra do Mar based on soil fractures and foliation, and the slope geometry of the area. This method could improve the diagnostic of hazard in hill slope areas and directly impact the development of a framework for safe hill slope use for agricultural and urban purposes.Carbohydrate consumption is a major public health concern nowadays, since it represents a large percentage of total energy intake in adults and children worldwide (Azaïs-Braesco et al. 2017, Wittekind andWalton 2014). Despite the development of nutritional guidelines that promotes the reduction of sugars in industrial processed foods, there are evidences that high sucrose western diets are linked to metabolic syndrome (Zhou et al. 2014), nonalcoholic fatty liver disease (Ishimoto et al. 2013), and cardiometabolic disease (DiNicolantonio and Lucan 2014). Unfortunately, the molecular effects of a high sucrose diet are less understood. Early efforts to understand the effects of carbohydrate metabolism and DNA damage were made using Saccharomyces cerevisiae as a model and a systems biology approach (Barea and Bonatto 2008), showing that reactive carbonyl species (RCS) provided by sugar metabolism could be linked to DNA damage. The manuscript of Franke et al. (2017) also addresses this question, and shows that the consumption of a high sucrose diet in rat model promotes DNA damage in hippocampus hence it contributes to the development of diabetes-associated neuropathologies.

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High consumption of sucrose induces DNA damage in male Wistar rats

Cited by 8 publications

References 26 publications

Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model

Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model

Metabolic programming in offspring of mice fed fructose during pregnancy and lactation

Landslide hazard mapping, DNA damage induced by sucrose and the biotechnology potential of sponge-associated bacteria communities

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