Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

D’Alpino, Paulo Henrique Perlatti; Moura, Gioconda Emanuella Diniz de Dantas; Barbosa, Silvana Coelho de Arruda; Marques, Lygia de Azevedo; Eberlin, Marcos N.; Nascimento, Fábio D.; Tersariol, Ivarne L.S.

doi:10.1016/j.dental.2017.09.011

Cited by 16 publications

(23 citation statements)

References 86 publications

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“…RU induces lower apoptosis than other SARCs, including PA (MaxCem Elite, G-Cem LinkAce, Bifix SE, and PA) and it also exhibits no significant difference in comparison to the negative control group, in accordance with our finding. 6 Furthermore, another recent study, in accordance with our results, reported that the main mechanism of RU-induced cell death is by necrotic, but not apoptotic, pathways. 18 PA exhibited only slight cytotoxicity in extract testing (92.1%) and culture inserts (98.4%) at a 24 h exposure period.…”

Section: Discussionsupporting

confidence: 91%

“…37 Kurt et al 37 reported that RU caused oxidative stress, DNA damage, and cytotoxicity compared to the negative control group. In contrast, RU did not show considerable cytotoxicity in other studies 6,33,36 as well as in the present investigation. Although a high biocompatibility of RU has been attributed to the absence of HEMA in its chemical composition, D'Alpino et al also reported that polymerized RU samples released HEMA.…”

Section: Discussioncontrasting

confidence: 60%

“…36 In a different study, in agreement with our results, the cytotoxicity of five SARCs was found in the following descending order: MaxCem Elite > G-Cem LinkAce > Bifix SE > PA > RU. 6 RU contains monomers shown to be toxic to fibroblast cells at certain concentrations such as TEGDMA and hydroxyethyl methacrylate (HEMA), 6,37 which may reduce levels of glutathione, cause oxidative stress, and induce cytotoxicity, apoptosis, or necrosis. 37 Kurt et al 37 reported that RU caused oxidative stress, DNA damage, and cytotoxicity compared to the negative control group.…”

Section: Discussionmentioning

confidence: 99%

“…Although a high biocompatibility of RU has been attributed to the absence of HEMA in its chemical composition, D'Alpino et al also reported that polymerized RU samples released HEMA. 6,36 On the other hand, RU additionally performs an acid-base reaction 38 during photopolymerization as well as the chemical polymerization, which may result in higher DC that can reduce the number of unreacted monomers. The manufacturer also reported that the pH of this material rises to a neutral level with the polymerization preventing further hydrolysis due to the self-neutralizing mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…5 Commonly used functional monomers in the composition of self-adhesive resin cements (SARCs) are 4-methacryloyloxyethy trimellitate anhydride (4-META), 10-methacryloyloxydecyl dihydrogen phosphate (MDP), glyceroldimethacrylate dihydrogen phosphate (GPDM), and 2-methacryloyloxyethyl phenyl phosphoric acid (Phenyl P). 6 Ionic and covalent interactions occur between enamel/dentin and SARC through functional monomers, thereby ensuring chemical bonding. 5 Self-adhesive resin cements (SARCs) simplify the cementation procedure by eliminating the multiple steps required for multi-step adhesive cementation.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

et al. 2020

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PURPOSE. The effects of four different self-adhesive resin cement materials on cell viability and apoptosis after direct and indirect exposure were evaluated using different cell culture techniques. MATERIALS AND METHODS. Self-adhesive cements were applied to NIH/3T3 mouse fibroblasts by the extract test method, cell culture inserts, and dentin barrier test method. After exposure periods of 24 h and 72 h, the cytotoxicity of these self-adhesive materials was evaluated using the MTT assay (viability) and the Annexin-V-FITC/PI staining (apoptosis). RESULTS. The lowest cell viability was found in cells exposed to BeautiCem SA for 24 h in the extract test method. Cell viability was reduced to 70.6% compared to negative controls. After the 72 h exposure period, viability rate of cell cultures exposed to BeautiCem SA decreased more than 2-fold (29.5%) while cells exposed to RelyX U200 showed the highest viability rate of 71.4%. In the dentin barrier test method, BeautiCem SA induced the highest number of cells in apoptosis after a 24 h exposure (4.1%). Panavia SA Cement Plus was the material that caused the lowest number of cells in apoptosis (1.5%). CONCLUSION. The used self-adhesive cements have showed different cytotoxic effects based on the evaluation method. As exposure time increased, the materials showed more cytotoxic and apoptotic effects. BeautiCem SA caused significantly more severe cytotoxic and apoptotic effects than other cements tested. Moreover, cements other than BeautiCem SA have caused necrotic cell death rather than apoptotic cell death.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

et al. 2020

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Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

et al. 2019

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OBJECTIVES This study evaluated the cytotoxicity of resin-based luting cements on fibroblast cells using different polymerization protocols. MATERIALS AND METHODS Two conventional dual-polymerized (RelyX ARC, VariolinkN) and two self-adhesive resin cements (RelyX Unicem, Multilink Speed) specimens were polymerized using four different polymerization protocols: (a) photopolymerization with direct light application, (b) photo-polymerization over ceramic and (c) resin nanoceramic discs and (d) auto-polymerization. The specimens were then assigned to four groups to test cytotoxicity at 0, 1, 2 and 7 preincubation days (n = 5). MTT test was performed using NIH/3T3 fibroblast cells. Data were analysed using three-and one-way ANOVA. Multiple comparisons were made using Bonferroni post hoc test (p < 0.05). RESULTS The highest cytotoxic values were recorded at day 2 for conventional resin cements and at day 0 for self-adhesive resin cements. Self-adhesive resin cements showed the most cytotoxic effect at the second day, while conventional resin cements presented immediate cytotoxicity. Auto-polymerized resin specimens and especially Multilink Speed demonstrated the most cytotoxic effect regardless of the preincubation time. Cytotoxicity of cements tested reached the lowest level at day 7. Interposition of ceramic or nano-ceramic restorative material did not significantly affect the cytotoxicity of tested luting cements (p > 0.05). CONCLUSIONS Cytotoxicity of dual-polymerized resin cements was material-dependent and decreased gradually up to 7 days. Photo-polymerization plays an important role in reducing the cytotoxic effects. CLINICAL RELEVANCE When luting ceramic or resin nano-ceramic restorations of which thickness does not exceed 2 mm, the level of cytotoxicity with the tested materials is not significant. Luting of restorative materials that do not allow for light transmission such as metal-fused porcelain, clinicians should be cautious in the use of dual-polymerized conventional resin cements as only auto-polymerization of resin cements takes place under such materials.

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In vitro cytotoxicity of different dental resin-cements on human cell lines

Diemer

Stark

Helfgen

et al. 2021

J Mater Sci: Mater Med

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Adhesive resin-cements are increasingly used in modern dentistry. Nevertheless, released substances from resin materials have been shown to cause cellular toxic effects. Disc-shaped specimens from 12 different resin cements and one conventional zinc phosphate cement were prepared and used for direct stimulation of five different human cell lines via transwell cell culture system or in an indirect way using conditioned cell culture media. Cytotoxicity was determined using LDH and BCA assays. All tested cements led to a decrease of cell viability but to a distinct extent depending on cell type, luting material, and cytotoxicity assay. In general, cements exhibited a more pronounced cytotoxicity in direct stimulation experiments compared to stimulations using conditioned media. Interestingly, the conventional zinc phosphate cement showed the lowest impact on cell viability. On cellular level, highest cytotoxic effects were detected in osteoblastic cell lines. All resin cements reduced cell viability of human cells with significant differences depending on cell type and cement material. Especially, osteoblastic cells demonstrated a tremendous increase of cytotoxicity after cement exposure. Although the results of this in vitro study cannot be transferred directly to a clinical setting, it shows that eluted substances from resin cements may disturb osteoblastic homeostasis that in turn could lead to conditions favoring peri-implant bone destruction. Thus, the wide use of resin cements in every clinical situation should be scrutinized. A correct use with complete removal of all cement residues and a sufficient polymerization should be given the utmost attention in clinical usage.

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Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

Cited by 16 publications

References 86 publications

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

In vitro cytotoxicity of different dental resin-cements on human cell lines

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