Differential antibody isotype expression to the major   antigen in juvenile and adult form paracoccidioidomycosis

Baida, Heide; Biselli, Paolo José Cesare; Juvenale, Michelangelo; Negro, G. Del; Mendes-Giannini, María José Soares; Duarte, Alberto J. S.; Benard, Gil

doi:10.1016/s1286-4579(99)80022-7

Cited by 59 publications

(50 citation statements)

References 29 publications

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“…After 5 months of treatment, as the clinical symptoms decline, the anti-Pb-1 antibody titers also decline. Similar patterns of immune response to gp43, the major exoantigen of P. brasiliensis, and to crude P. brasiliensis extract were observed in the sera of patients with chronic PCM (1,10). Also, 3 of 10 patients in the T1-4 group showed values of IgE above the cutoff (Fig.…”

Section: Discussionsupporting

confidence: 68%

Expression of Antibodies Directed to Paracoccidioides brasiliensis Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment

Bertini¹,

Colombo

Takahashi³

et al. 2007

Clin Vaccine Immunol

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Paracoccidioidomycosis (PCM) is a granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis. The immunoglobulin classes and isotypes of antibodies directed to acidic glycosphingolipids (GSLs) and glucosylceramide of P. brasiliensis were determined by enzyme-linked immunosorbent assay of sera from 31 PCM patients. The reactivities of 38 serum samples were analyzed by considering the stage of treatment: before antifungal treatment (n ‫؍‬ 10), during 1 to 4 months of treatment (T1-4; n ‫؍‬ 9), during 5 to 12 months of treatment (T5-12; n ‫؍‬ 9), and posttreatment (PT; n ‫؍‬ 10). Sera from healthy subjects (n ‫؍‬ 12) were used as controls. Only the GSL Pb-1 antigen, which presents the carbohydrate structure Galf␤1-6(Man␣1-3)Man␤1, was reactive with the PCM patient sera. The PCM patient sera did not react with Pb-2, which lacks the Galf residue and which is considered the biosynthetic precursor of Pb-1, indicating that the Galf residue is essential for antibody reactivity. The Pb-1 glycolipid from nontreated patients elicited a primary immune response with immunoglobulin M (IgM) production and subsequent switching to IgG1 production. The IgG1 titer increased after the start of antifungal treatment (T1-4 group), and general decreases in the anti-Pb-1 antibody titers were observed after 5 months of treatment (T5-12 and PT groups). The Pb-1 antigen, an acidic GSL with terminal Galf residue, has potential application as an elicitor of the host immune response in patients with PCM.

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Section: Discussionsupporting

confidence: 68%

Expression of Antibodies Directed to Paracoccidioides brasiliensis Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment

Bertini¹,

Colombo

Takahashi³

et al. 2007

Clin Vaccine Immunol

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“…Accordingly, these patients are characterized by severe immunosuppression as evidenced by the failure to respond to P. brasiliensis antigens in terms of delayed-type hypersensitivity and lymphoproliferative response in vitro. 28,29 Baida and others 30 investigated the antibody response to the major P. brasiliensis antigen (gp-43-kDa) in juvenile and adult clinical forms of PCM. The IgG4 isotype was detected in all patients with the JF of PCM but only in 12% of the patients with the AF of PCM.…”

Section: Resultsmentioning

confidence: 99%

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

Mamoni¹,

Rossi²,

Camargo³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved.

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“…Such IgG subclass switch has been reported in a variety of diseases, such as in malaria, 20 leprosy and tuberculosis, 21 and paracoccidioidomycosis. 22 A Recent study by Segawa et al 23 from Japan, describes the IgG subclass distribution in 16 pediatric patients with 'idiopathic' membranous glomerulonephritis. These authors found that in biopsies with segmental glomerular distribution of the deposits IgG1 and IgG3 dominated in the subepithelial deposits, whereas in biopsies with global glomerular distribution of deposits IgG4 dominance was noted with deposition of other IgG subclasses as well.…”

Section: Discussionmentioning

confidence: 99%

IgG subclass staining in renal biopsies with membranous glomerulonephritis indicates subclass switch during disease progression

et al. 2013

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Recent breakthrough findings revealed that most patients with idiopathic (primary) membranous glomerulonephritis have IgG4 antibodies to the phospholipase A2 receptor (PLA2R). These IgG4 antibodies can be detected in the glomerular immune complexes and they colocalize with PLA2R. In secondary forms of membranous glomerulonephritis, such IgG4 antibodies are absent or less prevalent. There are no studies addressing the IgG subclass distribution across different stages of membranous glomerulonephritis. During a 25-month period, we identified 157 consecutive biopsies with membranous glomerulonephritis with adequate tissue for light, immunofluorescence and electron microscopy. Of the 157 membranous glomerulonephritis cases, 114 were primary membranous glomerulonephritis and 43 were secondary membranous glomerulonephritis. We compared the intensity of IgG subclass staining (on a semiquantitative scale of 0 to 3 þ ) and the IgG subclass dominance between primary and secondary membranous glomerulonephritis and between the different stages of membranous glomerulonephritis. In primary membranous glomerulonephritis most (76% of cases) were IgG4 dominant. In contrast, in secondary membranous glomerulonephritis IgG1 was dominant in 60% of biopsies (P ¼ 0.0018). Interestingly, in early stage (stage 1) primary membranous glomerulonephritis, IgG1 was the dominant IgG subclass (64% of cases); in all later stages IgG4 dominated (P ¼ 0.0493). It appears that there is an inverse relationship between the intensity of glomerular capillary IgG4 and C1q staining. In secondary forms of membranous glomerulonephritis (heterogeneous group with low case numbers), we did not find such associations. Our data indicate that in early stage membranous glomerulonephritis, antibody response is different from later stages, with IgG1 dominant deposits. It is possible that early on, antigens other than PLA2R have an important role, Alternately, there may be an IgG subclass switch in the antibody response with IgG4 taking over later as the dominant immunoglobulin.

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Differential antibody isotype expression to the major antigen in juvenile and adult form paracoccidioidomycosis

Cited by 59 publications

References 29 publications

Expression of Antibodies Directed to Paracoccidioides brasiliensis Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment

Expression of Antibodies Directed to Paracoccidioides brasiliensis Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

IgG subclass staining in renal biopsies with membranous glomerulonephritis indicates subclass switch during disease progression

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