Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

Abstract

Abstract. Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved.

Humoral responses against the C-terminal region of merozoite surface protein 1 can be remembered for more than 30�years in persons exposed to Plasmodium vivax

“…Dotted line represents the cut-off value, which is the mean value plus 3·SD of antibody titers of healthy individuals in their twenties and thirties in a malaria-free area IgM. Antibody capture ELISA is known to be a better method for determining the specific isotype, avoiding competition from other isotype antibodies and has been used in IgE detection assay in parasite disease (Mamoni et al 2001). By using an antibody capture assay, the minor isotype antibodies against PvMSP1c could be determined (Fig.…”

Tropical dermatology: Fungal tropical diseases

“…83 Several other immunologic assays, particularly immunodiffusion, are valuable diagnostic adjuncts. 84,85 The intradermal reaction ( paracoccidioidine) is not reliable for diagnosis in endemic areas. 59,74,85 Slow-acting sulfonamides are the drugs of choice in the treatment of PM.…”

“…84,85 The intradermal reaction ( paracoccidioidine) is not reliable for diagnosis in endemic areas. 59,74,85 Slow-acting sulfonamides are the drugs of choice in the treatment of PM. 58 Both sulfamethoxypyridazine and sulfadimethoxine, 0.5 g/d, following an initial dose of 1 g/d for 1 week are effective, but relapse is common.…”

Negative Immunodiffusion Test Results Obtained with Sera of Paracoccidioidomycosis Patients May Be Related to Low-Avidity Immunoglobulin G2 Antibodies Directed against Carbohydrate Epitopes

“…However, due to the time and material required to perform these techniques, serologic tests have been developed to detect both antibodies (7,19,30,31) and antigens (14,15,16,22). Serologic tests are important tools when other procedures are not available as well as for posttherapy follow-up (21).…”