Differential activation and maturation of two porcine DC populations following TLR ligand stimulation

Auray, Gaël; Facci, Marina; Kessel, Jill van; Buchanan, Rachelle; Babiuk, Lorne A.; Gerdts, Volker

doi:10.1016/j.molimm.2010.03.016

Cited by 25 publications

(24 citation statements)

References 51 publications

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“…CpG ODN has been used previously to stimulate pig immune system (Auray et al, 2010; Dar et al, 2010). Overall, there were significant differences in the whole blood gene expression between DPL and Landrace pigs.…”

Section: Discussionmentioning

confidence: 99%

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

Yang

Wang

et al. 2016

Front. Microbiol.

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Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) mimic the immunostimulatory activity of microbial DNA by interacting with Toll-like receptor 9 (TLR9) to activate both the innate and adaptive immune responses in different species. However, few studies have been published to compare the effects of CpG ODN on different pig breeds. Therefore, in this study, whole blood gene expression profiles of DPL and Landrace pigs treated with CpG ODN were studied using RNA-seq technology. Five Hundred differentially expressed genes (DEGs) were identified between the two breeds. DPL pigs had significantly higher number of immune-relevant DEGs than the Landrace pigs after CpG ODN treatment. Pathway analysis showed that cytokine-cytokine receptor interaction and chemokine signaling pathway were the major enriched pathways of the immune-relevant DEGs. Further in vitro experiments showed that PBMCs of the DPL pigs had significantly higher levels of TLR9 mRNA than those of the Landrace pigs, both before and after CpG ODN stimulation. Cytokine and chemokine induction in the PBMCs of both breeds were also measured after CpG ODN stimulation. Our data showed that mRNA levels of cytokines (IFNα, IL8, IL12 p40) and chemokines (CXCL9, CXCL13) were significantly higher in the PBMCs of the DPL pigs than those of the Landrace pigs. Taken together, our data provide new information regarding the pig breed difference in response to CpG ODN stimulation and that higher levels of TLR9 mRNA in DPL pigs may be a major contributor for disease resistance.

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Section: Discussionmentioning

confidence: 99%

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

Yang

Wang

et al. 2016

Front. Microbiol.

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“…The ETEC-induced maturation of porcine DCs is mainly induced by flagellin, through Fcγ receptor ligation [49], among other bacterial constituents such as F4 and LPS [49,50]. We observed that ETEC up-regulated mRNA level of CCR7, receptor for CCL19 and CCL21, which provides DC signal for entry into the inductive sites of the adaptive immune system such as the lymph node.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, IL10 mRNA was increased in challenged DCs, probably to limit over-whelming specific and unspecific immune response and to avoid tissue damage [51]. Although no phenotypic study was performed in this work, published data [50] indicate that porcine monocyte-derived DC are expected to increase cell surface co-stimulatory molecules CD80/C86 and protein TNFα, IL6 and CXCL8 secretion after TLR ligand recognition.…”

Section: Discussionmentioning

confidence: 99%

Effect of Saccharomyces cerevisiae var. Boulardii and beta-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)

Badía

Zanello

Chevaleyre

et al. 2012

Vet Res

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Probiotic and prebiotics, often called "immune-enhancing" feed additives, are believed to deal with pathogens, preventing the need of an immune response and reducing tissue damage. In this study, we investigated if a recently developed β-galactomannan (βGM) had a similar protective role compared to Saccharomyces cerevisiae var. Boulardii (Scb), a proven probiotic, in the context of enterotoxigenic Escherichia coli (ETEC) infection. ETEC causes inflammation, diarrhea and intestinal damage in piglets, resulting in large economic loses worldwide. We observed that Scb and βGM products inhibited in vitro adhesion of ETEC on cell surface of porcine intestinal IPI-2I cells. Our data showed that Scb and βGM decreased the mRNA ETEC-induced gene expression of pro-inflammatory cytokines TNF-α, IL-6, GM-CSF and chemokines CCL2, CCL20 and CXCL8 on intestinal IPI-2I. Furthermore, we investigated the putative immunomodulatory role of Scb and βGM on porcine monocyte-derived dendritic cells (DCs) per se and under infection conditions. We observed a slight up-regulation of mRNA for TNF-α and CCR7 receptor after co-incubation of DC with Scb and βGM. However, no differences were found in DC activation upon ETEC infection and Scb or βGM co-culture. Therefore, our results indicate that, similar to probiotic Scb, prebiotic βGM may protect intestinal epithelial cells against intestinal pathogens. Finally, although these products may modulate DC activation, their effect under ETEC challenge conditions remains to be elucidated.

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“…Mature DCs can also synthesize high levels of IL-12 which enhances both innate and acquired immunity. LPS is one of the most potent activators of DCs (Auray et al, 2010). Most DCs in peripheral tissues in situ are of the immature phenotype including splenic DCs which consitutes 1-1.5% spleen cells (Bonifaz et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Tumor-derived CD4+CD25+ Tregs Inhibit the Maturation and Antigen-Presenting Function of Dendritic Cells

Chen²,

Lin³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Differential activation and maturation of two porcine DC populations following TLR ligand stimulation

Cited by 25 publications

References 51 publications

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

Effect of Saccharomyces cerevisiae var. Boulardii and beta-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)

Tumor-derived CD4+CD25+ Tregs Inhibit the Maturation and Antigen-Presenting Function of Dendritic Cells

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