Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

Esposito-Polesi, Natalia Pimentel; Oliveira, Leandro Silva de; Baccarin, Francisco José Benedini; Almeida, Cristina Vieira de; Almeida, Marcílio de

doi:10.1007/s11676-018-0816-1

Cited by 4 publications

(6 citation statements)

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“…The competence or recalcitrance to somatic embryogenesis of Eucalyptus depends on the genetic factor [32,101,102]. However, accurate regulation of chromatin modifications in response to developmental and environmental stimuli regulates the precise temporal and spatial gene expression [53,100,103].…”

Section: Role Of Cytokinins In Micropropagation Of Eucalyptusmentioning

confidence: 99%

“…Nonetheless, constant disclosure of the Eucalyptus explant to the PGRs (especially auxins such as NAA and 2,4-D) has an adverse impact on the physiology and morphology of propagated plants, such as fasciation, dwarfism, hyperhydricity, somaclonal variation, or structure distortion [38,82,103]. During SE initiation, auxins play an important role in a stress component and auxin signaling and could change the auxin content in Eucalyptus [27,103,120]. The positive interaction of the oxidation of callogenic tissue with embryogenic calli formation has been reported in cotyledonary of E. urophylla × E. grandis [52].…”

Section: Role Of Auxins In Somatic Embryogenesismentioning

confidence: 99%

“…It has been reported that WPM is the optimum media for organogenesis, adventitious buds, CB shoots multiplication, and callus formation in E. benthamii [130,131]. Reportedly, the induction of higher CB shoots was due to the essential abilities of each organ or tissue in the WPM media [27,103]. However, trunk base shoots of E. grandis were found to grow and multiply better in JADS media due to higher dry mass, a low rate of oxidation, and improved morphological feathers [132,133].…”

Section: Effect Of Mediamentioning

confidence: 99%

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The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Abiri

Atabaki

Hamid

et al. 2020

Forests

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Micropropagation is a reliable technique in biotechnology and genetic engineering domain, which has been widely applied for rapid mass propagation of plants in vitro condition. Through micropropagation techniques, reproduction of plants can be attained from different explants using organogenesis and somatic embryogenesis. Over the decades, micropropagation techniques have offered tremendous potential for forest tree improvement. Eucalyptus is a woody plant species recalcitrant to in vitro culture. In general, the micropropagation of Eucalyptus culture processes and the genotype, environment surroundings, and age of explants in culture media is frequently linked with the occurrence of micropropagation variation. In the current review paper, an update of the most important physiological and molecular phenomena aspects of Eucalyptus micropropagation was linked to the most profound information. To achieve the mentioned target, the effect of plant growth regulators (PGRs), nutrients, other adjuvant and environmental features, as well as genetic interaction with morpho- and physiological mechanisms was studied from the induction to plant acclimatisation. On the other hand, important mechanisms behind the organogenesis and somatic embryogenesis of Eucalyptus are discussed. The information of current review paper will help researchers in choosing the optimum condition based on the scenario behind the tissue culture technique of Eucalyptus. However, more studies are required to identify and overcome some of the crucial bottlenecks in this economically important forest species to establish efficient micropropagation protocol at the industrial level.

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Section: Role Of Cytokinins In Micropropagation Of Eucalyptusmentioning

confidence: 99%

Section: Role Of Auxins In Somatic Embryogenesismentioning

confidence: 99%

Section: Effect Of Mediamentioning

confidence: 99%

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The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Abiri

Atabaki

Hamid

et al. 2020

Forests

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“…Several similar studies have indicated the efficiency of this vegetative rescue method using epicormic shoots, such as in Ilex paraguariensis (Wendling et al 2013), Eucalyptus benthamii (Baccarin et al 2015), E. cloeziana (Oliveira et al 2015) and E. pilularis (Avelar et al 2020). According to Kuppusamy et al (2019) and Esposito-Polesi et al (2020), the use of this technique is especially important for germplasm conservation and the selection of superior individuals, in which a vegetative rescue is typically performed at the adult stage and propagation may be limited due to the ontogenetic age. Kratz et al (2016) reported that in situations in which vegetative rescues are performed in plants with high genetic value, the application of this method is indicated, considering the low interference of branch collection from the selected plant and ensuring the highest possible survival of the selected individuals.…”

Section: Discussionmentioning

confidence: 95%

Emission of epicormic shoots and in vitro establishment of Cordia trichotoma selected adult trees

et al. 2022

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Cordia trichotoma, popularly known in Brazil as 'louro-pardo', has numerous environmental and timber-related applications. This species is naturally propagated by seeds; however, this form of propagation results in restricted seedling production due to high genetic variability associated with a low degree of forest improvement. Thus, the cloning of selected adult trees is an alternative for producing high-quality plants. The aims of this work were to evaluate the emission of epicormic shoots from pruned branches and to define a protocol for the in vitro establishment of C. trichotoma selected adult trees. Propagules used in these experiments were derived from the selection of four C. trichotoma adult trees. The branches were placed in a greenhouse to induce epicormic shoots. Number of shoots according to the climatic season (autumn and spring) and shoot collection time were evaluated. Three sodium hypochlorite concentrations were evaluated for five minutes (C 1 = 0.58 % active chlorine, C 2 = 1.12 % active chlorine, and C 3 = 2.12 % active chlorine) for in vitro establishment. Results indicated that the epicormic shoot technique could be considered viable for shoot emission of C. trichotoma, regardless of the climatic season (autumn or spring). Sodium hypochlorite solution applied for 5 minutes (1.12 % of active chlorine) was effective for the in vitro establishment of nodal segments from epicormic shoots, resulting in 61.3 % of established explants. These results are promising for the cloning of adult trees during the mature stage of development in the field without the need to cut the selected tree.

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“…Nesse contexto, verifica-se que o desenvolvimento in vitro depende de diferentes fatores e da interação destes, e a resposta pode variar não somente de acordo com a espécie, mas entre genótipos da mesma espécie e até mesmo entre fontes de explantes de um mesmo genótipo (Cordeiro et al 2014;Esposito-Polesi et al 2018).…”

Section: Introductionunclassified

Propagação clonal de >i<Pinus elliottii>/i< x >i<Pinus caribaea>/i<

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Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

Cited by 4 publications

References 26 publications

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Emission of epicormic shoots and in vitro establishment of Cordia trichotoma selected adult trees

Propagação clonal de >i<Pinus elliottii>/i< x >i<Pinus caribaea>/i<

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