Differences in polyphosphate accumulation and phosphate adsorption by Acinetobacter isolates from wastewater producing polyphosphate: AMP phosphotransferase

Bark, Kerstin; Sponner, Alexander; Kämpfer, Peter; Grund, S.; Dott, Wolfgang

doi:10.1016/0043-1354(92)90131-m

Cited by 23 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, the spectrum of polyphosphate-dependent enzymes in the coryneform organisms is very heterogeneous in comparison to the Gramnegative isolates studied previously [4,5,12,22]. A most interesting result was the detection of the polyphosphate glucokinase.…”

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 60%

“…Electronmicroscopic pictures of all isolates showed electron-dense granules which could be identified as polyphosphate granules by the ESI technique as reported by Bark et al [4]. For isolates Nos.…”

Section: Polyphosphate Content and Page Of Polyphosphate Fractionsmentioning

confidence: 79%

“…2, 7, 14, 101, 103 and 136 (Fig. 3); however, as pointed out elsewhere [4], the polyphosphate standards of 'defined' chain length cannot serve as markers for exact chain length determination of the polyphosphates. Therefore, the amount of stored polyphosphates could not be quantified, and the application of electron microscopy was the only way for qualitative detection of polyphosphate granules ( Figs.…”

Section: Polyphosphate Content and Page Of Polyphosphate Fractionsmentioning

confidence: 99%

“…Again, it should be pointed out that the overall process, often simply described as 'biological' phosphate removal from wastewater, is a complex combination of enzyme-mediated biological mechanisms (as reported here and elsewhere [1,4,12,22]), chemical mechanisms including phosphate adsorption at the outer membrane structures [4,20] of microorganisms and phosphate precipitation [1,2]. The exact 'biological' proportion is still to be investigated.…”

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 99%

See 3 more Smart Citations

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Bark

Kämpfer

Sponner

et al. 1993

FEMS Microbiology Letters

View full text Add to dashboard Cite

Eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera Curtobacterium and Aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. All isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mU mg -1. Polyphosphate:AMP phosphotransferase could only be detected in one isolate. Three isolates showed a polyphosphate kinase activity also in minor amounts from 15 to 17 mU mg -1. A polyphosphate dependent NAD or 3-phosphoglycerate kinase could not be detected. Polyphosphate glucokinase activity was measured in cell-free extracts of nine isolates ranging from 2 to 376 mU mg-1. Three isolates showed in addition to the polyphosphate glucokinase, a glucose-6-phosphate-dependent NAD kinase. For the regeneration of NADP from NAD and polyphosphate, this enzyme system may give the isolates a distinct competitive advantage, especially for anabolic processes. The polyphosphate-dependent enzymes reported here may play an additional role in the complex process of 'biological' phosphate removal from wastewater.

show abstract

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 60%

Section: Polyphosphate Content and Page Of Polyphosphate Fractionsmentioning

confidence: 79%

Section: Polyphosphate Content and Page Of Polyphosphate Fractionsmentioning

confidence: 99%

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 99%

See 2 more Smart Citations

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Bark

Kämpfer

Sponner

et al. 1993

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…Since ADP is a substrate for adenylate kinase as well, a complete inhibition of this enzyme is essential to obtain the true activity of PPK. The relatively high values for PPK reported by Bayly and colleagues may be overestimations as a result of the incomplete inhibition of adenyl ate kinase by Pl,p5-di(adenosine-51)pentaphosphate (see also Bark et al, 1992).…”

Section: Regulator Of Development and Survivalmentioning

confidence: 91%

Ecological Aspects of Biological Phosphorus Removal in Activated Sludge Systems

Kortstee

Appeldoorn

Bonting

et al. 2000

Advances in Microbial Ecology

View full text Add to dashboard Cite

Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in Acinetobacter johnsonii 120 and some other bacteria from activated sludge in batch and continuous culture

Weltin

Hoffmeister

Dott

et al. 1996

Acta Biotechnologica

View full text Add to dashboard Cite

Twelve bacterial isolates, four of them assigned to the genus Acinetobacter. were taken from sewage of a treatment plant with Enhanced Biological Phosphorus Removal (EBPR) and screened for phosphorus uptake, polyphosphate (polyp) accumulation and adsorption under limited carbon and nitrogen conditions. In addition, poly-J-hydroxyalkanoate (PHA) production was studied under carbon. nitrogen, phosphorus, and oxygen limitation. Under C limitation, the uptake of phosphorus was highest, ranging up to 66 mg P per g dry weight (dw) for the Acinetobacter isolates, whereas the highest amount of polyp was detected under limited N conditions (up to 25 mg PolyPIg dw). Extra-cellular polyp was detected, however to a minor extent, accounting for a maximum of 10% of the total polyp in one Acinetobacter isolate. The highest PHA concentration (given as 3-hydroxybutyrate, 3 HB) with 211 mg 3 H B / g dry weight (21% of the dried cell mass) was found for A. johnsonii 120 under nitrogen limitation. but also under P and 9 limitation, PHA, mainly poly-$3-hydroxybutyrate and poly-P-hydroxyvaler, were produced. Three isolates, assigned to the genus Pseudomonas, showed even higher values (345427 mg 3 HB / g dw) under N limitation. Studies with Acinetobacter johnsonii 120 in continuous culture, simulating the aerobic/anaerobic periods of a waste-water matment plant, resulted in a P elimination of 36% at an anaerobic contact time of 0.6 h. This value increased to 51% at an anaerobic contact time of 3.1 h. No release of phosphate and no uptake of acetate could be detected during the anaerobic period. In addition, Acinetobacter johnsonii 120 was not able to synthesize PHA under anaerobic conditions. By changing the anaerobic conditions to aerobic, a continuous decrease of the polyp content relative to the totalp content from 45% (day 1 of the aerobic process) to 19% (day 17 of the aerobic process) was observed. The amount of PHA increased to 50.4 mg 3 HB / g dw under aerobic conditions. The results indicate again that the EPBR process cannot be defined by simply applying the knowledge of the metabolic processes, observed or assumed in Acinetobacter pure cultures, to the complexity of the process in sewage treatment plants.

show abstract

Differences in polyphosphate accumulation and phosphate adsorption by Acinetobacter isolates from wastewater producing polyphosphate: AMP phosphotransferase

Cited by 23 publications

References 43 publications

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Ecological Aspects of Biological Phosphorus Removal in Activated Sludge Systems

Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in Acinetobacter johnsonii 120 and some other bacteria from activated sludge in batch and continuous culture

Contact Info

Product

Resources

About