Differences in erythrocytes size and shape in prediabetes and diabetes assessed by two microscopy techniques and its association with dietary patterns. Pilot study

Loyola-Leyva, Alejandra; Loyola-Rodríguez, Juan Pablo; Barquera, Sı́món; González, Francisco; Camacho‐López, Santiago; Terán-Figueroa, Yolanda

doi:10.1002/jemt.24238

Cited by 2 publications

(3 citation statements)

References 27 publications

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“…The volume of patients’ cells was significantly higher as compared to those of NPC and PC cells, mainly as a result of the enlarged diameter. Our results showed that the ratio of the maximum height and the depth of concavity, H/h 2 , determined for the biconcave shape [ 28 ] had values close to those for PC and NPC cells, indicating a maximally high surface-to-volume ratio and an H/h 2 ratio for PE cells that was twice as high ( Table 3 ).…”

Section: Resultsmentioning

confidence: 65%

“…RBC roughness is an important indicator of membrane integrity and cell deformability [ 48 ]. Many studies have revealed the dependence of the modifications of the RBC surface roughness from various disorders or aging conditions [ 18 , 22 , 28 , 49 ]. Our results showed a faster decrease in the roughness value (calculated at 1 × 1 µm 2 scanned area) of cell membranes from patients, most dramatically expressed on the 10th day, during which period enhanced protrusions were detected in patient RBCs and which is most likely related to the vesiculation process presented in Figure 3 H and Figure 4 A–D.…”

Section: Discussionmentioning

confidence: 99%

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Membrane Lesions and Reduced Life Span of Red Blood Cells in Preeclampsia as Evidenced by Atomic Force Microscopy

Giosheva

Strijkova

Komsa-Penkova

et al. 2023

IJMS

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Preeclampsia (PE) presents with maternal de novo hypertension and significant proteinuria and is one of the leading causes of maternal and perinatal morbidity and mortality with unknown etiology. The disease is associated with inflammatory vascular response and severe red blood cell (RBC) morphology changes. This study examined the nanoscopic morphological changes of RBCs from PE women versus normotensive healthy pregnant controls (PCs) and non-pregnant controls (NPCs) applying atomic force microscopy (AFM) imaging. The results revealed that the membrane of fresh PE RBCs differed significantly from healthy ones by the presence of invaginations and protrusions and an increased roughness value (Rrms) (4.7 ± 0.8 nm for PE vs. 3.8 ± 0.5 nm and 2.9 ± 0.4 nm for PCs and NPCs, respectively). PE-cells aging resulted in more pronounced protrusions and concavities, with exponentially increasing Rrms values, in contrast to the controls, where the Rrms parameter decreased linearly with time. The Rrms, evaluated on a 2 × 2 µm2 scanned area, for senescent PE cells (13 ± 2.0 nm) was significantly higher (p < 0.01) than that of PCs (1.5 ± 0.2 nm) and NPCs (1.9 ± 0.2 nm). Furthermore, the RBCs from PE patients appeared fragile, and often only ghosts were observed instead of intact cells at 20–30 days of aging. Oxidative-stress simulation on healthy cells led to RBC membrane features similar to those observed for PE cells. The results demonstrate that the most pronounced effects on RBCs in PE patients are related to impaired membrane homogeneity and strongly altered roughness values, as well as to vesiculation and ghost formation in the course of cell aging.

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Section: Resultsmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Membrane Lesions and Reduced Life Span of Red Blood Cells in Preeclampsia as Evidenced by Atomic Force Microscopy

Giosheva

Strijkova

Komsa-Penkova

et al. 2023

IJMS

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“…The biomechanical properties of cells reflect their function and state. Therefore, cell biomechanics play a major role in disease diagnosis and treatment (Loyola et al, 2022; Teck, Chwee, 2005). The effects of G‐Rg1 on hepatocyte biomechanics have not been widely reported.…”

Section: Resultsmentioning

confidence: 99%

Attenuation of alcohol‐induced hepatocyte damage by ginsenoside Rg1 evaluated using atomic force microscopy

Zhang

Weng

Wang

et al. 2023

Microscopy Res & Technique

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Alcoholic liver disease is an important cause of death worldwide. Hepatocyte apoptosis is commonly observed in alcoholic liver disease. In this study, we investigated the effect of ginsenoside Rg1 (G-Rg1), an organic component of ginseng, on the alcoholinduced morphological and biophysical properties of hepatocytes. Human hepatocytes (HL-7702) were treated in vitro with alcohol and G-Rg1. The cell morphology was observed using scanning electron microscopy. Cell height, roughness, adhesion, and elastic modulus were detected using atomic force microscopy. We found that alcohol significantly induced hepatocyte apoptosis, whereas G-Rg1 attenuated the alcohol-induced hepatocyte damage. Scanning electron microscopy revealed that alcohol-induced significant morphological changes in hepatocytes, including decreased cell contraction, roundness, and pseudopods, whereas G-Rg1 inhibited these negative changes. Atomic force microscopy revealed that alcohol increased the cell height and decreased the adhesion and elastic modulus of hepatocytes. Following treatment with G-Rg1, the cell height, adhesion, and elastic modulus of alcoholinjured hepatocytes were all similar to those of normal cells. Thus, G-Rg1 can attenuate the alcohol-induced damage to hepatocytes by modulating the morphology and biomechanics of the cells. Research Highlights• In this study, the morphological characteristics of hepatocytes were observed using SEM.• The changes in hepatocyte three-dimensional images and biomechanical action caused by alcohol and G-Rg1 were examined at the nanoscale using AFM under near-physiological conditions.• Alcohol-induced hepatocytes showed abnormal morphology and biophysical properties. G-Rg1 attenuated the alcohol-induced damage to hepatocytes by modulating the morphology and biomechanics of the cells.

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Differences in erythrocytes size and shape in prediabetes and diabetes assessed by two microscopy techniques and its association with dietary patterns. Pilot study

Cited by 2 publications

References 27 publications

Membrane Lesions and Reduced Life Span of Red Blood Cells in Preeclampsia as Evidenced by Atomic Force Microscopy

Membrane Lesions and Reduced Life Span of Red Blood Cells in Preeclampsia as Evidenced by Atomic Force Microscopy

Attenuation of alcohol‐induced hepatocyte damage by ginsenoside Rg1 evaluated using atomic force microscopy

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